FastQCFastQC Report
Thu 26 May 2016
SRR1545640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545640_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1886225
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC62340.3305013982955374No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.25065938581028246No Hit
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG44430.2355498416148657No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC30210.16016116847141776No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT28550.15136052167689432Illumina Single End Adapter 2 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT28080.1488687722832642No Hit
ATGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC27570.14616495911145277No Hit
ATTGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC24210.12835160174422458No Hit
ATGTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC22300.11822555633606807No Hit
ATATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC19950.10576680936791741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA302.1662454E-645.0000041
GTACGAC302.1662454E-645.00000445
ACGCGAA253.8917602E-545.014
CGAACTA253.8917602E-545.044
ATCTACG800.045.01
CAACGTT406.8193913E-945.024
GTTTCGA253.8917602E-545.035
CGTGAAT253.8917602E-545.043
ATTACGC351.2124838E-745.01
ATTACGA351.2124838E-745.01
TAGCGAT253.8917602E-545.02
CGCGATA351.2124838E-745.043
TAAGTCG207.034439E-445.09
CCTCGTA253.8917602E-545.033
CGACACG207.034439E-445.021
AACGGAT351.2124838E-745.025
GCCGATT253.8917602E-545.043
ACGCTAG253.8917602E-545.037
ACTCGAT406.8193913E-945.017
CGTAACG207.034439E-445.03