FastQCFastQC Report
Thu 26 May 2016
SRR1545639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545639_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1502354
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT79470.5289698699507573Illumina Single End Adapter 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC44380.2954030807652524No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC42870.2853521873007294No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT41820.2783631554214253Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC40900.27223943225098746No Hit
TTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC30560.20341410879193586No Hit
GGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC29440.1959591414540115No Hit
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC28440.18930292061657905No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT22140.14736872934075457Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC21980.14630373400676538No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC21230.14131156837869105No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC19130.1273335046200829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA206.3120754E-446.00000437
TCGCAAT405.6134013E-946.00000415
CGACGTA206.3120754E-446.00000413
CGCGATC206.3120754E-446.00000418
CACGACA206.3120754E-446.00000428
TCAATCG206.3120754E-446.00000439
TATTACG405.6134013E-946.0000041
CATACCG206.3120754E-446.00000426
TTAACGA206.3120754E-446.00000413
TCGTAGT206.3120754E-446.00000432
TCGTAAG405.6134013E-946.0000041
CCGACGT206.3120754E-446.00000412
TTAGTCG206.3120754E-446.0000041
ACGTACG206.3120754E-446.00000440
CACGTAA206.3120754E-446.00000421
TCGAACG206.3120754E-446.0000041
CCGCAAC206.3120754E-446.00000410
ATCGATT206.3120754E-446.00000427
TCGCATA253.41766E-546.011
CTCACGA301.8617866E-646.024
Produced by FastQC (version 0.11.5)