##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545639_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1502354
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55305740191726	33.0	31.0	34.0	31.0	34.0
2	32.96806145555575	34.0	31.0	34.0	31.0	34.0
3	33.0458493803724	34.0	33.0	34.0	31.0	34.0
4	36.423614540913796	37.0	37.0	37.0	35.0	37.0
5	36.402736638635105	37.0	37.0	37.0	35.0	37.0
6	36.4044645935645	37.0	37.0	37.0	35.0	37.0
7	36.461371287992044	37.0	37.0	37.0	35.0	37.0
8	36.49156190884439	37.0	37.0	37.0	35.0	37.0
9	38.28954360956206	39.0	39.0	39.0	37.0	39.0
10	38.08426243082523	39.0	39.0	39.0	35.0	39.0
11	37.925561485508744	39.0	38.0	39.0	35.0	39.0
12	37.56368405848422	39.0	37.0	39.0	35.0	39.0
13	37.492489786029125	39.0	37.0	39.0	35.0	39.0
14	38.730602774046595	41.0	38.0	41.0	35.0	41.0
15	38.82354425122175	41.0	38.0	41.0	35.0	41.0
16	38.89361894733199	41.0	38.0	41.0	35.0	41.0
17	38.867569827084694	41.0	38.0	41.0	35.0	41.0
18	38.835040875852165	41.0	38.0	41.0	35.0	41.0
19	38.82547255839835	40.0	38.0	41.0	35.0	41.0
20	38.726276230502265	40.0	38.0	41.0	35.0	41.0
21	38.67814975698138	40.0	38.0	41.0	35.0	41.0
22	38.80334927720098	40.0	38.0	41.0	35.0	41.0
23	38.80178706217043	40.0	38.0	41.0	35.0	41.0
24	38.74745898769531	41.0	38.0	41.0	35.0	41.0
25	38.67121929984544	40.0	38.0	41.0	35.0	41.0
26	38.54348043137636	40.0	37.0	41.0	35.0	41.0
27	38.430812578127394	40.0	37.0	41.0	35.0	41.0
28	38.38873993745815	40.0	37.0	41.0	35.0	41.0
29	38.29877379099733	40.0	36.0	41.0	35.0	41.0
30	38.219542131881035	40.0	36.0	41.0	34.0	41.0
31	38.17873151068257	40.0	36.0	41.0	34.0	41.0
32	38.07874043001849	40.0	36.0	41.0	34.0	41.0
33	38.027132087377545	40.0	36.0	41.0	34.0	41.0
34	37.87583552212062	40.0	35.0	41.0	34.0	41.0
35	37.743372068101124	40.0	35.0	41.0	34.0	41.0
36	37.67577947674117	40.0	35.0	41.0	33.0	41.0
37	37.593675658333524	40.0	35.0	41.0	33.0	41.0
38	37.46870378086656	40.0	35.0	41.0	33.0	41.0
39	37.390669575878924	40.0	35.0	41.0	33.0	41.0
40	37.26812855026179	39.0	35.0	41.0	33.0	41.0
41	37.13721998942992	39.0	35.0	41.0	33.0	41.0
42	37.031347472033886	39.0	35.0	41.0	33.0	41.0
43	36.9240318859603	39.0	35.0	41.0	33.0	41.0
44	36.83544357721283	39.0	35.0	41.0	32.0	41.0
45	36.76450290677164	39.0	35.0	41.0	32.0	41.0
46	36.75470694656519	39.0	35.0	41.0	32.0	41.0
47	36.711952708882194	38.0	35.0	41.0	32.0	41.0
48	36.61834228151288	38.0	35.0	40.0	32.0	41.0
49	36.50975136352684	38.0	35.0	40.0	32.0	41.0
50	36.3991582543129	38.0	35.0	40.0	32.0	41.0
51	36.270211281761824	38.0	35.0	40.0	31.0	41.0
52	35.93064484136229	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	10.0
13	14.0
14	24.0
15	61.0
16	104.0
17	140.0
18	249.0
19	534.0
20	843.0
21	1303.0
22	1920.0
23	2892.0
24	3755.0
25	4896.0
26	6111.0
27	7823.0
28	9697.0
29	12318.0
30	15565.0
31	20150.0
32	26382.0
33	37216.0
34	87856.0
35	148140.0
36	91185.0
37	135613.0
38	235453.0
39	651349.0
40	741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.6917424255535	13.33613782104617	46.30326807130676	12.668851682093567
2	26.72672352854254	13.279559943927996	47.06434036185879	12.929376165670675
3	27.001425762503377	14.311141049313278	42.93442158106544	15.753011607117894
4	23.8061735117023	14.640690542974557	45.90456044314456	15.64857550217858
5	24.40456776498748	15.860576135850804	43.144159099652946	16.590696999508772
6	25.214962651944884	19.385178193688038	42.93242471481422	12.467434439552862
7	84.69854641449352	3.21901495919071	7.1117060293379595	4.970732596977809
8	85.31863994770873	3.5565519178569103	7.367571158328863	3.7572369761054984
9	77.66618253753775	5.586832397690557	9.523654211989983	7.223330852781701
10	43.85597535600797	21.164452585742108	15.858446145182825	19.121125913067093
11	35.30373001303288	22.946189779506028	24.12813491360891	17.621945293852182
12	28.37240756838934	24.192899942357126	26.465599985090066	20.969092504163466
13	23.381306935648986	24.79169356889255	32.882929056667074	18.944070438791393
14	18.671231946665035	30.42205765085992	31.134472967090314	19.77223743538474
15	18.788248308987097	22.79349607349533	36.50704161602392	21.911214001493658
16	23.378444760688893	22.0917972728132	33.31964370581101	21.210114260686897
17	24.2940744990861	22.01158981172214	27.10366531456634	26.590670374625418
18	24.59653317393903	22.467607501294633	29.18626369018221	23.749595634584125
19	26.714143271159795	24.315507530182636	27.45072066903007	21.519628529627504
20	27.418238311343394	22.846546153569662	25.582985102046525	24.152230433040415
21	26.353109852937457	24.614438407991727	28.47704335995378	20.555408379117036
22	29.07364043361285	20.65591731376227	26.03966841370276	24.23077383892212
23	27.651538851695406	24.148236700537957	23.513566043688773	24.686658404077868
24	26.20041614692676	20.955380689238357	27.72575571403278	25.11844744980211
25	28.906702415010045	23.374317903769683	23.882720051332775	23.836259629887497
26	23.565085192970496	25.623654611363232	25.122840555554816	25.688419640111455
27	23.14554359358713	25.83046339278226	25.78580015096309	25.238192862667518
28	24.803608204191555	23.301365723391424	27.9997257637015	23.895300308715523
29	23.33810806241405	23.269482425580122	28.63805734201127	24.754352169994558
30	23.77748519989297	26.19296117958883	28.779768283640205	21.249785336877995
31	28.45893843927596	23.815891594124952	25.182080921007966	22.54308904559112
32	27.762498053055406	22.74563784567419	27.525336904617685	21.96652719665272
33	29.251228405555548	22.980469316818805	26.068489849928845	21.699812427696802
34	27.62990613397375	22.849474890738136	26.293802925275934	23.226816050012182
35	24.676474386196595	24.528573159188845	28.736303161571776	22.058649293042784
36	29.399994941272162	23.112062802774847	24.419011764204708	23.068930491748283
37	28.285344199835727	24.661364764895623	25.641293596582432	21.41199743868622
38	25.109128740629703	23.966588433884425	29.11504212722168	21.80924069826419
39	24.739109424276833	24.63520581700451	25.46903060130968	25.156654157408976
40	27.159577569600774	22.110700939991506	28.05696926290342	22.672752227504304
41	25.45132505388211	22.690990272598867	26.89292936285323	24.964755310665794
42	24.596067238480412	24.11548809401779	25.578991369544063	25.70945329795774
43	22.998574237496623	21.28686048694249	28.911761142846494	26.802804132714392
44	26.939123535465008	21.73568945801056	26.7654627338164	24.55972427270803
45	22.729995726706225	22.814729417966735	27.843903633897206	26.611371221429835
46	23.0131513611306	23.068198307456164	26.72911976804402	27.189530563369217
47	24.47725369653224	22.843617416401194	30.237280960412793	22.44184792665377
48	23.66133414627977	21.359213607445383	32.372263794019254	22.607188452255595
49	23.711655175810762	20.751234396154302	28.143633258206783	27.393477169828152
50	22.421280204266107	22.825312809098257	30.375530667206263	24.377876319429376
51	25.12783272118289	23.266354001786528	27.399134957539967	24.206678319490614
52	23.765304315760467	21.331989664220284	28.118938678899912	26.783767341119336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	233.0
1	183.0
2	133.0
3	125.5
4	118.0
5	159.5
6	201.0
7	332.0
8	463.0
9	542.0
10	621.0
11	737.5
12	854.0
13	1081.0
14	1377.5
15	1447.0
16	1377.0
17	1307.0
18	1394.0
19	1481.0
20	2105.0
21	2729.0
22	2796.5
23	2864.0
24	4524.0
25	6184.0
26	7230.0
27	8276.0
28	10415.5
29	12555.0
30	13816.0
31	15077.0
32	19535.0
33	23993.0
34	27101.5
35	30210.0
36	32676.5
37	35143.0
38	37489.5
39	48512.0
40	57188.0
41	64190.5
42	71193.0
43	79116.5
44	87040.0
45	98733.5
46	110427.0
47	128798.5
48	147170.0
49	151787.0
50	156404.0
51	139376.0
52	122348.0
53	108316.5
54	94285.0
55	89307.5
56	84330.0
57	80105.5
58	75881.0
59	75753.5
60	75626.0
61	69917.5
62	64209.0
63	56330.0
64	43415.5
65	38380.0
66	33458.5
67	28537.0
68	24963.0
69	21389.0
70	18166.5
71	14944.0
72	11470.5
73	7997.0
74	6675.5
75	5354.0
76	4401.0
77	3448.0
78	2443.0
79	1438.0
80	943.5
81	449.0
82	390.5
83	332.0
84	237.0
85	142.0
86	171.5
87	201.0
88	136.0
89	46.0
90	21.0
91	21.5
92	22.0
93	18.0
94	14.0
95	14.5
96	15.0
97	13.5
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1502354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.64907383205426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3610175982072	12.241577206571735
2	12.263052520250497	4.328630386725202
3	5.153735367951002	2.728759680595097
4	2.7104696510836024	1.9134911596606736
5	1.6677709242369179	1.4717306088405369
6	1.0639533397129082	1.1266674627872295
7	0.7377456888370869	0.9114369692095833
8	0.5323307239065063	0.7516115399437456
9	0.4039246019036113	0.6416005609421964
>10	3.21935981598903	12.419410329268388
>50	1.2725195127576483	16.467983101426757
>100	1.6046941849983989	41.556880159522116
>500	0.004147470872881172	0.45909094234326464
>1k	0.004901556486132295	2.4523002474610798
>5k	3.770428066255611E-4	0.5288296447024097
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	7947	0.5289698699507573	Illumina Single End Adapter 2 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	4438	0.2954030807652524	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	4287	0.2853521873007294	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	4182	0.2783631554214253	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	4090	0.27223943225098746	No Hit
TTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3056	0.20341410879193586	No Hit
GGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2944	0.1959591414540115	No Hit
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2844	0.18930292061657905	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	2214	0.14736872934075457	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2198	0.14630373400676538	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2123	0.14131156837869105	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1913	0.1273335046200829	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5559941265507331	0.0
2	0.0	0.0	0.0	1.1157822989788027	0.0
3	0.0	0.0	0.0	3.3846217336260294	0.0
4	0.0	0.0	0.0	3.881042683681742	0.0
5	0.0	0.0	0.0	4.641382789941652	0.0
6	0.0	0.0	0.0	6.958280139035141	0.0
7	0.0	0.0	0.0	7.255014463967879	0.0
8	0.0	0.0	0.0	7.792703983215674	0.0
9	0.0	0.0	0.0	8.14694805618383	0.0
10	0.0	0.0	0.0	9.304198610979835	0.0
11	0.0	0.0	0.0	10.326660693817836	0.0
12	0.0	0.0	0.0	10.767968135339608	0.0
13	0.0	0.0	0.0	11.00672677677831	0.0
14	0.0	0.0	0.0	11.179988205176675	0.0
15	6.656220837432455E-5	0.0	0.0	11.407431271191744	0.0
16	6.656220837432455E-5	0.0	0.0	11.917963409422812	0.0
17	6.656220837432455E-5	0.0	0.0	12.554431245898105	0.0
18	6.656220837432455E-5	0.0	0.0	13.251870065244276	0.0
19	6.656220837432455E-5	0.0	0.0	13.72246487845075	0.0
20	6.656220837432455E-5	0.0	0.0	14.218220206422721	0.0
21	6.656220837432455E-5	0.0	0.0	14.914860279268401	0.0
22	6.656220837432455E-5	0.0	0.0	15.533223195065876	0.0
23	6.656220837432455E-5	0.0	0.0	16.13660961397913	0.0
24	6.656220837432455E-5	0.0	0.0	16.62497653682155	0.0
25	6.656220837432455E-5	0.0	0.0	17.08139359964429	0.0
26	6.656220837432455E-5	0.0	0.0	17.485426204476443	0.0
27	6.656220837432455E-5	0.0	0.0	17.90270468877508	0.0
28	6.656220837432455E-5	0.0	0.0	18.28284146080085	0.0
29	6.656220837432455E-5	0.0	0.0	18.73812696608123	0.0
30	6.656220837432455E-5	0.0	0.0	19.1747750530168	0.0
31	6.656220837432455E-5	0.0	0.0	19.598310384902625	0.0
32	6.656220837432455E-5	0.0	0.0	20.019715726120474	0.0
33	6.656220837432455E-5	0.0	0.0	20.437127334835864	0.0
34	6.656220837432455E-5	0.0	0.0	20.869448878227104	0.0
35	6.656220837432455E-5	0.0	0.0	21.360544851612868	0.0
36	6.656220837432455E-5	0.0	0.0	21.7713668016992	0.0
37	6.656220837432455E-5	0.0	0.0	22.20774863980127	0.0
38	6.656220837432455E-5	0.0	0.0	22.656444486452592	0.0
39	6.656220837432455E-5	0.0	0.0	23.344298347792865	0.0
40	6.656220837432455E-5	0.0	0.0	23.762708389633868	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAA	20	6.3120754E-4	46.000004	37
TCGCAAT	40	5.6134013E-9	46.000004	15
CGACGTA	20	6.3120754E-4	46.000004	13
CGCGATC	20	6.3120754E-4	46.000004	18
CACGACA	20	6.3120754E-4	46.000004	28
TCAATCG	20	6.3120754E-4	46.000004	39
TATTACG	40	5.6134013E-9	46.000004	1
CATACCG	20	6.3120754E-4	46.000004	26
TTAACGA	20	6.3120754E-4	46.000004	13
TCGTAGT	20	6.3120754E-4	46.000004	32
TCGTAAG	40	5.6134013E-9	46.000004	1
CCGACGT	20	6.3120754E-4	46.000004	12
TTAGTCG	20	6.3120754E-4	46.000004	1
ACGTACG	20	6.3120754E-4	46.000004	40
CACGTAA	20	6.3120754E-4	46.000004	21
TCGAACG	20	6.3120754E-4	46.000004	1
CCGCAAC	20	6.3120754E-4	46.000004	10
ATCGATT	20	6.3120754E-4	46.000004	27
TCGCATA	25	3.41766E-5	46.0	11
CTCACGA	30	1.8617866E-6	46.0	24
>>END_MODULE