FastQCFastQC Report
Thu 26 May 2016
SRR1545638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545638_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1726334
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC64390.3729869191014022No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.2790885193711066No Hit
ATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG43590.25250038520935114No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT31540.1826992922574658Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC25900.15002890518289047No Hit
ATATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC25110.1454527339437212No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC24990.14475761932511322No Hit
ATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT24350.14105034135920397No Hit
ATTGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC22250.1288858355335642No Hit
ATGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC19860.1150414693796218No Hit
ATTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC18810.10895921646680191No Hit
ATGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC18080.10473060253693665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGT253.8916216E-545.00000420
GTACCGT650.045.00000420
TCCGCTA502.1827873E-1145.0000042
TCCGCGC253.8916216E-545.0000042
CAAGTCG253.8916216E-545.00000432
TCCGATA253.8916216E-545.0000042
CTATCGC253.8916216E-545.00000426
CGACCCG502.1827873E-1145.00000434
CGACCCA253.8916216E-545.00000419
TCCTCCG253.8916216E-545.00000439
TAACGAA253.8916216E-545.00000439
TGACGGA502.1827873E-1145.00000419
CCGTCGT253.8916216E-545.00000444
TCGGACA253.8916216E-545.00000438
TGATTCG502.1827873E-1145.00000417
GTCCGCT253.8916216E-545.00000416
CGTACGA650.045.00000424
ACGTCAT253.8916216E-545.00000441
TCATCGG253.8916216E-545.00000417
ACGTCAC253.8916216E-545.00000428