##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545638_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1726334
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993674456970666	31.0	31.0	34.0	31.0	34.0
2	32.678398849817015	34.0	31.0	34.0	31.0	34.0
3	32.60626970215497	34.0	31.0	34.0	31.0	34.0
4	36.033632541559165	37.0	35.0	37.0	35.0	37.0
5	35.784676661642536	37.0	35.0	37.0	35.0	37.0
6	35.77976046350243	37.0	35.0	37.0	35.0	37.0
7	35.77900916045215	37.0	35.0	37.0	35.0	37.0
8	35.74526424202964	37.0	35.0	37.0	33.0	37.0
9	37.9894394711568	39.0	39.0	39.0	35.0	39.0
10	37.99786078476124	39.0	39.0	39.0	35.0	39.0
11	37.77192536322635	39.0	38.0	39.0	35.0	39.0
12	37.21942856944253	39.0	37.0	39.0	33.0	39.0
13	36.90537230918235	39.0	37.0	39.0	33.0	39.0
14	37.7480991511492	40.0	36.0	41.0	33.0	41.0
15	37.64169911500324	40.0	36.0	41.0	33.0	41.0
16	37.72357434888034	40.0	36.0	41.0	33.0	41.0
17	37.81835670270063	40.0	36.0	41.0	33.0	41.0
18	37.86936826824937	40.0	36.0	41.0	33.0	41.0
19	37.841649993570186	40.0	36.0	41.0	33.0	41.0
20	37.77624318353227	40.0	36.0	41.0	33.0	41.0
21	37.79034821766819	40.0	36.0	41.0	33.0	41.0
22	37.72524957511119	40.0	35.0	41.0	33.0	41.0
23	37.6851321934226	40.0	35.0	41.0	33.0	41.0
24	37.64423512483679	40.0	35.0	41.0	33.0	41.0
25	37.59914303952769	40.0	35.0	41.0	33.0	41.0
26	37.496634486721575	40.0	35.0	41.0	32.0	41.0
27	37.389897319985586	39.0	35.0	41.0	32.0	41.0
28	37.324396669474154	39.0	35.0	41.0	32.0	41.0
29	37.273948726028685	39.0	35.0	41.0	32.0	41.0
30	37.22684254611217	39.0	35.0	41.0	32.0	41.0
31	37.22507869276745	39.0	35.0	41.0	32.0	41.0
32	37.109155007084375	39.0	35.0	41.0	31.0	41.0
33	36.98258506175514	39.0	35.0	41.0	31.0	41.0
34	36.93643987779885	39.0	35.0	41.0	31.0	41.0
35	36.86632134917113	39.0	35.0	41.0	31.0	41.0
36	36.811140254435124	39.0	35.0	41.0	31.0	41.0
37	36.65327277340306	39.0	35.0	41.0	30.0	41.0
38	36.57061785262875	39.0	35.0	41.0	30.0	41.0
39	36.550900347209755	39.0	35.0	41.0	30.0	41.0
40	36.45492529255637	39.0	35.0	41.0	30.0	41.0
41	36.451372098330914	39.0	35.0	41.0	30.0	41.0
42	36.37353779743665	39.0	35.0	41.0	30.0	41.0
43	36.27567724438029	38.0	35.0	41.0	30.0	41.0
44	36.18554520735848	38.0	35.0	40.0	30.0	41.0
45	36.14323126347509	38.0	35.0	40.0	30.0	41.0
46	36.133862856202796	38.0	35.0	40.0	30.0	41.0
47	36.05384126130865	38.0	35.0	40.0	30.0	41.0
48	36.00206449041727	38.0	35.0	40.0	29.0	41.0
49	35.98303283142196	38.0	35.0	40.0	29.0	41.0
50	35.90425780874385	38.0	35.0	40.0	29.0	41.0
51	35.70824243744258	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	5.0
12	9.0
13	18.0
14	20.0
15	28.0
16	52.0
17	132.0
18	312.0
19	591.0
20	1170.0
21	1994.0
22	3093.0
23	4849.0
24	7213.0
25	10964.0
26	14989.0
27	18654.0
28	21707.0
29	26270.0
30	32485.0
31	40134.0
32	50029.0
33	64068.0
34	99486.0
35	193609.0
36	117169.0
37	157476.0
38	254305.0
39	605170.0
40	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.1646066172594645	93.46806585515897	2.41494403748058	2.952383490100989
2	3.054101929290624	1.85584017924689	93.13145660109805	1.9586012903644372
3	25.303272715476844	15.276707751802373	45.03369568113702	14.38632385158376
4	25.360330040420916	15.926581993982625	46.731628989523465	11.981458976072997
5	23.320747896988646	14.148073316055873	49.14078040518231	13.390398381773169
6	19.978520958285014	14.830849650183566	50.719849113786786	14.47078027774463
7	21.72320072477284	16.40024468034575	48.181116747975764	13.695437846905639
8	25.0431260694628	19.159675937564806	44.33151406390652	11.46568392906587
9	85.44204076383828	3.2000759991983014	7.929172454461303	3.4287107825021113
10	87.22246100696621	2.9715570683309256	7.209091635801647	2.5968902889012204
11	79.87608423398949	6.654621875025343	8.290805834792108	5.178488056193066
12	39.39961791866464	33.22277149149585	13.642551209673215	13.735059380166295
13	29.022773113429963	24.880643027363185	29.85615761492272	16.24042624428413
14	27.737506183623793	25.30657450991523	29.30116651818246	17.654752788278515
15	20.159945873741698	31.123293638426862	31.873611942995968	16.843148544835472
16	16.52183181238393	36.329991762891765	28.85866813722026	18.28950828750404
17	15.613664563172597	26.797711219265796	38.76497827187554	18.82364594568606
18	19.38970095010583	25.10029924684331	36.62663192638273	18.88336787666813
19	19.43876445693591	23.53785536286721	30.374539341749628	26.648840838447253
20	19.246623191109023	25.48881039242696	34.42821609259853	20.836350323865485
21	22.803061284780348	26.59392678357722	30.313311329093906	20.28970060254852
22	24.772668556606078	25.300318478347762	28.833759863386803	21.093253101659354
23	22.698794091989154	27.206844098534816	30.200702760879413	19.89365904859662
24	26.164288023059267	23.170487286932886	27.420244286447463	23.244980403560376
25	23.2136423195048	26.49041263162285	26.267454617704338	24.028490431168013
26	22.3590568221445	24.389139065789124	33.81234454051186	19.43945957155452
27	22.535210451743406	27.58197428771026	29.873419627951485	20.009395632594853
28	20.416269389353392	30.09064294626648	28.157471265699453	21.335616398680674
29	19.26898271134091	29.1074612444637	28.753068641410064	22.870487402785326
30	17.87626264674159	27.7265002021625	33.62611174894314	20.77112540215277
31	18.786920723336273	25.156082194986602	32.20610843556345	23.85088864611367
32	19.42584691027345	29.03667540580212	30.583595063295977	20.953882620628452
33	22.07504457422492	27.895355128266026	29.98753427783963	20.042066019669427
34	22.796052212376054	27.62709881170156	27.2883462875666	22.288502688355788
35	23.25251081192863	27.53169433029761	27.253069220672245	21.96272563710151
36	21.67680182398076	26.13677306940604	29.439436401067233	22.746988705545974
37	20.268325828026327	26.653880419432163	30.222830576238437	22.854963176303077
38	23.861720848920314	28.363572750116724	27.8989465537955	19.875759847167465
39	22.277960116640234	29.391125935073976	27.98432979944785	20.34658414883794
40	20.18207368910072	27.18697540568627	31.382976874695164	21.24797403051785
41	20.38047098649508	28.623429765039678	28.698096660321816	22.298002588143433
42	23.29572377071876	25.85218155930428	29.101494843987318	21.750599825989642
43	21.74654499071443	25.870891727788482	29.478420745927497	22.904142535569594
44	20.849152018091516	27.323217870933437	27.663186845650955	24.164443265324092
45	20.11256222723992	26.005859816234867	29.31101397527941	24.570563981245808
46	21.895183666660103	26.937660962478873	28.37405739561406	22.79309797524697
47	18.906248732863975	29.65353170359849	28.023951332708503	23.41626823082903
48	19.95691447888995	29.143085868667363	27.480371700957058	23.419627951485634
49	20.675141658566652	27.50597508940912	30.43043814232935	21.388445109694878
50	21.17330713523571	23.79168805109556	32.45327960869681	22.58172520497192
51	19.806943499925275	25.4782678207114	29.541212766475084	25.173575912888236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1095.0
1	1117.0
2	1139.0
3	4457.5
4	7776.0
5	5305.0
6	2834.0
7	2869.0
8	2904.0
9	3154.5
10	3405.0
11	3509.0
12	3613.0
13	3877.5
14	4142.0
15	4197.5
16	4253.0
17	4301.0
18	4349.0
19	4602.5
20	4856.0
21	7003.0
22	9150.0
23	10252.5
24	11355.0
25	14845.0
26	24442.0
27	30549.0
28	35075.5
29	39602.0
30	43520.5
31	47439.0
32	56034.0
33	64629.0
34	70419.0
35	76209.0
36	84965.5
37	93722.0
38	100242.0
39	106762.0
40	121534.0
41	136306.0
42	144021.5
43	151737.0
44	157811.0
45	163885.0
46	165546.5
47	167208.0
48	169286.5
49	171365.0
50	149790.0
51	128215.0
52	102889.5
53	77564.0
54	69274.5
55	60985.0
56	54090.5
57	47196.0
58	41016.5
59	34837.0
60	27127.5
61	19418.0
62	16260.5
63	13103.0
64	10094.5
65	7086.0
66	5957.5
67	4829.0
68	3449.0
69	2069.0
70	1650.0
71	1231.0
72	934.5
73	638.0
74	471.5
75	190.0
76	75.0
77	64.0
78	53.0
79	60.5
80	68.0
81	44.5
82	21.0
83	15.0
84	9.0
85	7.0
86	5.0
87	4.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1726334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.576326012041891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.43801984846931	10.815892347903937
2	12.042293541895093	3.7514938028252924
3	5.381622848402451	2.514777358817102
4	3.0866339381276298	1.923136660004348
5	1.9112379752967386	1.4885032894908432
6	1.2133971533734724	1.134016178581728
7	0.8412639307895544	0.9172660873704962
8	0.6016122761124457	0.7496727156459213
9	0.41957287968702306	0.5881863563834671
>10	2.5842708488676123	8.245512087776309
>50	0.7254386777624209	8.260848726782587
>100	1.7359776796720592	55.66220410295488
>500	0.010448704864750101	1.0843962132430303
>1k	0.007836528648562574	2.4898216013221055
>5k	3.7316803088393213E-4	0.3742724708979348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	6439	0.3729869191014022	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4818	0.2790885193711066	No Hit
ATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	4359	0.25250038520935114	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	3154	0.1826992922574658	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2590	0.15002890518289047	No Hit
ATATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	2511	0.1454527339437212	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	2499	0.14475761932511322	No Hit
ATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	2435	0.14105034135920397	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	2225	0.1288858355335642	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	1986	0.1150414693796218	No Hit
ATTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	1881	0.10895921646680191	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	1808	0.10473060253693665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20239420645135878	0.0
2	0.0	0.0	0.0	0.8029153107104419	0.0
3	0.0	0.0	0.0	1.174338221919976	0.0
4	0.0	0.0	0.0	1.5321484718484373	0.0
5	0.0	0.0	0.0	2.9230728236830186	0.0
6	0.0	0.0	0.0	3.2451425969713856	0.0
7	0.0	0.0	0.0	3.7289423715225443	0.0
8	0.0	0.0	0.0	5.299148368739769	0.0
9	0.0	0.0	0.0	5.481442177469713	0.0
10	5.792621821733222E-5	0.0	0.0	5.851996195405987	0.0
11	5.792621821733222E-5	0.0	0.0	6.14550834311321	0.0
12	5.792621821733222E-5	0.0	0.0	6.961399126704334	0.0
13	1.7377865465199665E-4	0.0	0.0	7.7533663821717	0.0
14	1.7377865465199665E-4	0.0	0.0	8.078332466370934	0.0
15	1.7377865465199665E-4	0.0	0.0	8.230446715409649	0.0
16	2.3170487286932888E-4	0.0	0.0	8.326198754122899	0.0
17	2.3170487286932888E-4	0.0	0.0	8.472752086212749	0.0
18	2.3170487286932888E-4	0.0	0.0	8.828071508757866	0.0
19	2.3170487286932888E-4	0.0	0.0	9.349581251368507	0.0
20	3.475573093039933E-4	0.0	0.0	9.909785707748327	0.0
21	4.6340974573865775E-4	0.0	0.0	10.279818389720646	0.0
22	5.2133596395599E-4	0.0	0.0	10.654543095368567	0.0
23	5.2133596395599E-4	0.0	0.0	11.215210961494126	0.0
24	5.2133596395599E-4	0.0	0.0	11.731391492028774	0.0
25	5.2133596395599E-4	0.0	0.0	12.277056467636042	0.0
26	5.792621821733222E-4	0.0	0.0	12.702292835569478	0.0
27	6.371884003906545E-4	0.0	0.0	13.074758418706924	0.0
28	6.371884003906545E-4	0.0	0.0	13.425385817576437	0.0
29	6.371884003906545E-4	0.0	0.0	13.747861074392326	0.0
30	7.530408368253189E-4	0.0	0.0	14.080473419396247	0.0
31	7.530408368253189E-4	0.0	0.0	14.447609790457697	0.0
32	8.688932732599832E-4	0.0	0.0	14.88159301734195	0.0
33	8.688932732599832E-4	0.0	0.0	15.258518919282132	0.0
34	9.268194914773155E-4	0.0	0.0	15.627914412854059	0.0
35	9.847457096946476E-4	0.0	0.0	15.985261253036782	0.0
36	9.847457096946476E-4	0.0	0.0	16.3422605359102	0.0
37	0.00104267192791198	0.0	0.0	16.795359414806175	0.0
38	0.0011005981461293122	0.0	0.0	17.18862051028364	0.0
39	0.0011005981461293122	0.0	0.0	17.588311415983235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGT	25	3.8916216E-5	45.000004	20
GTACCGT	65	0.0	45.000004	20
TCCGCTA	50	2.1827873E-11	45.000004	2
TCCGCGC	25	3.8916216E-5	45.000004	2
CAAGTCG	25	3.8916216E-5	45.000004	32
TCCGATA	25	3.8916216E-5	45.000004	2
CTATCGC	25	3.8916216E-5	45.000004	26
CGACCCG	50	2.1827873E-11	45.000004	34
CGACCCA	25	3.8916216E-5	45.000004	19
TCCTCCG	25	3.8916216E-5	45.000004	39
TAACGAA	25	3.8916216E-5	45.000004	39
TGACGGA	50	2.1827873E-11	45.000004	19
CCGTCGT	25	3.8916216E-5	45.000004	44
TCGGACA	25	3.8916216E-5	45.000004	38
TGATTCG	50	2.1827873E-11	45.000004	17
GTCCGCT	25	3.8916216E-5	45.000004	16
CGTACGA	65	0.0	45.000004	24
ACGTCAT	25	3.8916216E-5	45.000004	41
TCATCGG	25	3.8916216E-5	45.000004	17
ACGTCAC	25	3.8916216E-5	45.000004	28
>>END_MODULE