FastQCFastQC Report
Thu 26 May 2016
SRR1545637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545637_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1434635
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT80510.5611880373753603Illumina Single End Adapter 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC51170.35667608834302805No Hit
GTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC49530.34524460925601286No Hit
TGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC40140.27979242106877356No Hit
ATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC37950.26452721423916187No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT36880.25706887117629224Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC31940.2226350256336978No Hit
GGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC29520.20576662356627295No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT22440.1564160918979392Illumina Single End Adapter 2 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC21090.14700603289338401No Hit
TCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC20290.1414297016314254No Hit
AGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC19110.13320461302003644No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC16490.11494212813712198No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT14480.10093159584145096Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC351.0198164E-746.00000418
AGCGTAA351.0198164E-746.00000414
CTACTCG351.0198164E-746.00000418
GAATGCG351.0198164E-746.00000442
CGCGATA351.0198164E-746.0000046
TCGGCAC351.0198164E-746.00000427
GTAGCGT351.0198164E-746.00000412
TACGCAC351.0198164E-746.00000434
CGTAGCG700.046.0000041
GTCGTTC551.8189894E-1246.00000415
ACGTCCA351.0198164E-746.00000433
GGCGATT351.0198164E-746.0000048
GCGCTTA351.0198164E-746.00000430
GATTCGC351.0198164E-746.00000416
CGTCCGC351.0198164E-746.00000427
CCGCCGT351.0198164E-746.00000438
TATCGGA351.0198164E-746.00000412
TCGAATC351.0198164E-746.00000435
GCGTAAA351.0198164E-746.00000415
TGGTCGA351.0198164E-746.00000434
Produced by FastQC (version 0.11.5)