##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545637_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1434635
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.593465236802395	33.0	31.0	34.0	31.0	34.0
2	32.98970121320057	34.0	33.0	34.0	31.0	34.0
3	33.04741763584466	34.0	33.0	34.0	31.0	34.0
4	36.411165906310664	37.0	37.0	37.0	35.0	37.0
5	36.401309043763746	37.0	37.0	37.0	35.0	37.0
6	36.41096585542664	37.0	37.0	37.0	35.0	37.0
7	36.5223830451648	37.0	37.0	37.0	35.0	37.0
8	36.58652549254688	37.0	37.0	37.0	35.0	37.0
9	38.38333583106505	39.0	39.0	39.0	37.0	39.0
10	38.109074433566725	39.0	39.0	39.0	35.0	39.0
11	37.899919491717405	39.0	38.0	39.0	35.0	39.0
12	37.63132085861561	39.0	37.0	39.0	35.0	39.0
13	37.55691238538025	39.0	37.0	39.0	35.0	39.0
14	38.94939339971491	41.0	38.0	41.0	35.0	41.0
15	38.98546389848289	41.0	38.0	41.0	35.0	41.0
16	39.04657630686551	41.0	38.0	41.0	35.0	41.0
17	39.006462270891205	41.0	38.0	41.0	35.0	41.0
18	38.983713627508045	41.0	38.0	41.0	35.0	41.0
19	38.99318223799085	41.0	38.0	41.0	35.0	41.0
20	38.91933767125436	41.0	38.0	41.0	35.0	41.0
21	38.90090650235077	41.0	38.0	41.0	35.0	41.0
22	38.970465658512445	41.0	38.0	41.0	35.0	41.0
23	38.97423456140412	41.0	38.0	41.0	35.0	41.0
24	38.94682340804456	41.0	38.0	41.0	35.0	41.0
25	38.87707605070279	41.0	38.0	41.0	35.0	41.0
26	38.77624831403109	40.0	38.0	41.0	35.0	41.0
27	38.70178268340031	40.0	38.0	41.0	35.0	41.0
28	38.64856496600181	40.0	38.0	41.0	35.0	41.0
29	38.68049155360074	40.0	38.0	41.0	35.0	41.0
30	38.634974052633595	40.0	37.0	41.0	35.0	41.0
31	38.55410052034141	40.0	37.0	41.0	35.0	41.0
32	38.54967082219519	40.0	37.0	41.0	35.0	41.0
33	38.49249669776633	40.0	37.0	41.0	35.0	41.0
34	38.44144468802169	40.0	37.0	41.0	35.0	41.0
35	38.28241259972049	40.0	37.0	41.0	35.0	41.0
36	38.2268381853224	40.0	37.0	41.0	35.0	41.0
37	38.1830437707152	40.0	36.0	41.0	34.0	41.0
38	38.07661321520805	40.0	36.0	41.0	34.0	41.0
39	38.06760674317858	40.0	36.0	41.0	34.0	41.0
40	38.01700781034897	40.0	36.0	41.0	34.0	41.0
41	37.901155346133336	40.0	36.0	41.0	34.0	41.0
42	37.802915724208596	40.0	36.0	41.0	34.0	41.0
43	37.7097916891753	40.0	35.0	41.0	33.0	41.0
44	37.64605631397533	40.0	35.0	41.0	33.0	41.0
45	37.59766769944969	40.0	35.0	41.0	33.0	41.0
46	37.61585002457071	40.0	35.0	41.0	33.0	41.0
47	37.62047907655954	40.0	35.0	41.0	33.0	41.0
48	37.54440467435968	40.0	35.0	41.0	33.0	41.0
49	37.485043233993316	39.0	35.0	41.0	33.0	41.0
50	37.37930344652124	39.0	35.0	41.0	33.0	41.0
51	37.17467160636678	39.0	35.0	40.0	33.0	41.0
52	36.80657797976489	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	11.0
12	12.0
13	17.0
14	17.0
15	22.0
16	21.0
17	47.0
18	93.0
19	201.0
20	358.0
21	603.0
22	1093.0
23	1754.0
24	2466.0
25	3250.0
26	4177.0
27	5660.0
28	7397.0
29	9784.0
30	12201.0
31	15948.0
32	20982.0
33	28866.0
34	62236.0
35	146740.0
36	70666.0
37	95855.0
38	192258.0
39	750966.0
40	933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.688161100210156	13.497928044415477	47.924733468791715	13.889177386582649
2	23.665671059189272	13.81124815719678	51.697330679929046	10.82575010368491
3	24.093375666981498	14.032140579311115	47.73451086861815	14.13997288508924
4	20.436557033670585	14.828022458674159	50.16920680173006	14.566213705925202
5	22.453934275965665	16.036831667985236	47.4926375001307	14.016596555918404
6	24.111986672568282	18.89741990122923	44.82896346457461	12.16162996162787
7	87.12878188528789	2.175396529430831	6.225485924991374	4.4703356602899
8	89.4662405420194	1.850226712717869	6.094511844476122	2.589020900786611
9	81.16175891428831	4.92640985337734	7.7074656619976505	6.204365570336705
10	39.57815054003283	32.33533268043789	12.334705343170912	15.751811436358377
11	28.69872824795157	23.2072269253155	31.179568322256184	16.91447650447675
12	27.79794163672293	25.371679904644733	28.569496770955677	18.260881687676655
13	20.436347921248263	29.347604094421232	33.24552935067108	16.97051863365943
14	16.203006339591603	37.13857531706671	28.411268371397604	18.247149971944083
15	15.260466948039047	26.84083407974851	37.73426690412544	20.164432068087006
16	19.364437644418267	25.836467115328986	35.2942037521739	19.504891488078847
17	19.13016202727523	23.866906913605202	29.133960902947443	27.86897015617213
18	18.678339786775034	25.96758060412579	32.90767338033716	22.44640622876202
19	21.79641511603997	27.39832779766282	30.125641713746003	20.679615372551208
20	24.378186786186035	24.514737197963246	27.67421678684822	23.4328592290025
21	22.065612507711023	28.126945181178485	30.1767348489337	19.63070746217679
22	25.857866286546756	22.781125512761086	26.971599047841437	24.389409152850725
23	22.86525841067589	27.15324803869974	24.711581691510386	25.269911859113993
24	21.93638103071513	23.806752240116825	32.460033388283435	21.796833340884618
25	24.501354002934544	26.845573961321172	27.349604603261458	21.303467432482826
26	20.38699738957993	29.621541367664943	27.278018450686066	22.713442792069063
27	20.35423644341592	27.88576885409878	27.19123679542183	24.568757907063468
28	18.796488305387783	26.964628633763986	32.83378699111621	21.40509606973202
29	18.896095522554518	25.130782394128122	31.064835306541383	24.908286776775974
30	19.80190083191892	28.28949523746458	29.020970490752003	22.887633439864498
31	20.795393950377623	27.583601403841396	30.1898392273993	21.431165418381678
32	22.654194272410752	28.154269204362087	25.009148668476648	24.182387854750512
33	23.743321472012045	26.395494324340333	26.54605526841322	23.315128935234398
34	22.22885960540486	24.815510565405138	29.304387527141053	23.651242302048953
35	20.44980082041774	24.84604097906436	29.821940772391585	24.882217428126317
36	24.534951398787843	26.73481408163052	27.333015017757127	21.397219501824505
37	23.842789280897232	28.112446719897395	26.564805682281555	21.479958316923817
38	19.3769146856169	25.871388889857	32.65750521909754	22.094191205428558
39	20.35096034879952	27.84478281932338	28.102060802921997	23.7021960289551
40	24.69750145507394	24.032663360366925	29.13152125802033	22.138313926538807
41	23.090263377095916	24.44642714000425	28.980890609806675	23.482418873093156
42	21.749364821017192	26.91499928553256	26.33973101172075	24.9959048817295
43	20.640581053717497	24.44377838265482	29.259079835637635	25.656560727990048
44	24.42433092737874	25.26698428520146	26.162682494153565	24.146002293266232
45	19.285253740498455	27.138749577418647	28.141722459022677	25.43427422306022
46	20.074513726487922	27.277739634122966	27.14244389687969	25.505302742509418
47	21.335461633098316	26.015955277823277	30.55794679482935	22.090636294249062
48	21.13032234679901	21.732914643794416	33.8428938371084	23.29386917229818
49	20.365319401799063	23.082456513329173	28.719500081902368	27.832724002969396
50	19.40897859037316	24.03294217693002	31.88344073579691	24.67463849689991
51	21.34926305297166	24.313710455969638	28.976568953078658	25.36045753798004
52	20.47440638211113	22.87912953469001	29.64726219561073	26.999201887588132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	295.0
2	196.0
3	174.5
4	153.0
5	214.5
6	276.0
7	282.5
8	289.0
9	485.0
10	681.0
11	752.0
12	823.0
13	985.0
14	1252.5
15	1358.0
16	1662.5
17	1967.0
18	1609.5
19	1252.0
20	2245.5
21	3239.0
22	3965.5
23	4692.0
24	7160.5
25	9629.0
26	11846.5
27	14064.0
28	16961.0
29	19858.0
30	23105.5
31	26353.0
32	31420.5
33	36488.0
34	41299.5
35	46111.0
36	50663.0
37	55215.0
38	57349.5
39	70330.5
40	81177.0
41	91146.0
42	101115.0
43	105604.5
44	110094.0
45	113548.0
46	117002.0
47	127652.5
48	138303.0
49	152575.5
50	166848.0
51	158246.5
52	149645.0
53	122537.5
54	95430.0
55	75691.0
56	55952.0
57	51608.5
58	47265.0
59	39552.0
60	31839.0
61	26634.0
62	21429.0
63	18228.0
64	11572.0
65	8117.0
66	6528.5
67	4940.0
68	3993.5
69	3047.0
70	2297.0
71	1547.0
72	1273.5
73	1000.0
74	845.0
75	690.0
76	464.0
77	238.0
78	169.5
79	101.0
80	72.5
81	44.0
82	45.5
83	47.0
84	36.0
85	25.0
86	18.0
87	11.0
88	11.5
89	10.0
90	8.0
91	7.0
92	6.0
93	4.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1434635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.293231501764673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.92907898715443	10.579045151340711
2	13.012970526541087	4.240466826291489
3	6.18215511759592	3.021818535324286
4	3.5275662511265384	2.299018142696673
5	2.2931628496900314	1.868151659062303
6	1.5161586613371636	1.4821874437543217
7	1.077863157118756	1.2293311762311185
8	0.7814064630336195	1.0185309119345505
9	0.5816239983307017	0.8528881006605701
>10	3.4987084090667775	11.467133250769871
>50	0.9311967324386405	11.132953252763924
>100	1.652275078653837	46.40449236189222
>500	0.009414672812842374	1.0327696869947254
>1k	0.005563215753043221	2.4530720448182843
>5k	8.558793466220341E-4	0.9181414554649301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	8051	0.5611880373753603	Illumina Single End Adapter 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	5117	0.35667608834302805	No Hit
GTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	4953	0.34524460925601286	No Hit
TGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	4014	0.27979242106877356	No Hit
ATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	3795	0.26452721423916187	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	3688	0.25706887117629224	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	3194	0.2226350256336978	No Hit
GGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2952	0.20576662356627295	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2244	0.1564160918979392	Illumina Single End Adapter 2 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2109	0.14700603289338401	No Hit
TCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2029	0.1414297016314254	No Hit
AGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	1911	0.13320461302003644	No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	1649	0.11494212813712198	No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	1448	0.10093159584145096	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5888605812628299	0.0
2	0.0	0.0	0.0	1.1617589142883034	0.0
3	0.0	0.0	0.0	3.7724578028557785	0.0
4	0.0	0.0	0.0	4.366755307099019	0.0
5	0.0	0.0	0.0	5.234432451459779	0.0
6	0.0	0.0	0.0	8.05856541907872	0.0
7	0.0	0.0	0.0	8.394957602456374	0.0
8	6.970414077448271E-5	0.0	0.0	9.079661377284118	0.0
9	6.970414077448271E-5	0.0	0.0	9.548630836414837	0.0
10	6.970414077448271E-5	0.0	0.0	11.053822052299017	0.0
11	6.970414077448271E-5	0.0	0.0	12.458290784764069	0.0
12	6.970414077448271E-5	0.0	0.0	13.035162253813688	0.0
13	6.970414077448271E-5	0.0	0.0	13.292161420849206	0.0
14	6.970414077448271E-5	0.0	0.0	13.435194317718445	0.0
15	6.970414077448271E-5	0.0	0.0	13.66960934314303	0.0
16	6.970414077448271E-5	0.0	0.0	14.266137379891052	0.0
17	6.970414077448271E-5	0.0	0.0	15.140575825906938	0.0
18	6.970414077448271E-5	0.0	0.0	16.09517403381348	0.0
19	6.970414077448271E-5	0.0	0.0	16.71686526538109	0.0
20	6.970414077448271E-5	0.0	0.0	17.324476260512256	0.0
21	6.970414077448271E-5	0.0	0.0	18.242688906934518	0.0
22	6.970414077448271E-5	0.0	0.0	19.056345342195055	0.0
23	6.970414077448271E-5	0.0	0.0	19.89600142196447	0.0
24	6.970414077448271E-5	0.0	0.0	20.55122034524461	0.0
25	6.970414077448271E-5	0.0	0.0	21.099791933139787	0.0
26	6.970414077448271E-5	0.0	0.0	21.62807961606959	0.0
27	6.970414077448271E-5	0.0	0.0	22.110641382651337	0.0
28	6.970414077448271E-5	0.0	0.0	22.59633983556793	0.0
29	6.970414077448271E-5	0.0	0.0	23.112917222847624	0.0
30	6.970414077448271E-5	0.0	0.0	23.696968218397014	0.0
31	6.970414077448271E-5	0.0	0.0	24.229368445632513	0.0
32	6.970414077448271E-5	0.0	0.0	24.742112105169607	0.0
33	6.970414077448271E-5	0.0	0.0	25.239660262017864	0.0
34	6.970414077448271E-5	0.0	0.0	25.718458004997785	0.0
35	6.970414077448271E-5	0.0	0.0	26.31219787611483	0.0
36	6.970414077448271E-5	0.0	0.0	26.838742955525273	0.0
37	6.970414077448271E-5	0.0	0.0	27.365218330794942	0.0
38	6.970414077448271E-5	0.0	0.0	27.875173824701058	0.0
39	2.7881656309793084E-4	0.0	0.0	28.36163902316617	0.0
40	2.7881656309793084E-4	0.0	0.0	28.860790375252243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	35	1.0198164E-7	46.000004	18
AGCGTAA	35	1.0198164E-7	46.000004	14
CTACTCG	35	1.0198164E-7	46.000004	18
GAATGCG	35	1.0198164E-7	46.000004	42
CGCGATA	35	1.0198164E-7	46.000004	6
TCGGCAC	35	1.0198164E-7	46.000004	27
GTAGCGT	35	1.0198164E-7	46.000004	12
TACGCAC	35	1.0198164E-7	46.000004	34
CGTAGCG	70	0.0	46.000004	1
GTCGTTC	55	1.8189894E-12	46.000004	15
ACGTCCA	35	1.0198164E-7	46.000004	33
GGCGATT	35	1.0198164E-7	46.000004	8
GCGCTTA	35	1.0198164E-7	46.000004	30
GATTCGC	35	1.0198164E-7	46.000004	16
CGTCCGC	35	1.0198164E-7	46.000004	27
CCGCCGT	35	1.0198164E-7	46.000004	38
TATCGGA	35	1.0198164E-7	46.000004	12
TCGAATC	35	1.0198164E-7	46.000004	35
GCGTAAA	35	1.0198164E-7	46.000004	15
TGGTCGA	35	1.0198164E-7	46.000004	34
>>END_MODULE