FastQCFastQC Report
Thu 26 May 2016
SRR1545636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545636_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1074745
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC77740.7233343723394852Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.3540374693531954No Hit
ATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG35790.3330092254441751No Hit
ATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT28450.2647139554033747No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT26370.24536052738091363Illumina Single End Adapter 2 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC26260.2443370287835719No Hit
ATATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC24040.22368096618267588No Hit
ATGTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC23560.21921479048518486No Hit
ATTGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC22340.20786326058739515No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC20630.19195250966508334Illumina Single End Adapter 2 (95% over 21bp)
ATTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC16500.15352478960125426No Hit
ATGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC16140.15017515782813598No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC15170.14114976110612285No Hit
ATGGTCTGGGGTGTCCTCCAGCCTCCACATGTATGGCATGGTACACACACA14100.13119391111379908No Hit
ATTTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG14090.13110086578676802No Hit
ATTGGTCTGGGAGATGGAGAAACTAGGGGCCAGGGAGAGAAAGGAGCCACA13300.12375028495131403No Hit
ATTTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT11300.10514121954510139No Hit
ATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC10880.10123331580979675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT453.8562575E-1045.00000435
CTCCGTA302.1653686E-645.00000443
CCTAGTT302.1653686E-645.00000415
CCCTATG453.8562575E-1045.00000425
CATGCCA302.1653686E-645.00000438
TCCGACA600.045.00000421
GCGCGAG900.045.0000043
GATCCTA600.045.00000428
CCGGAAT600.045.00000436
CGCGCCC302.1653686E-645.00000438
ATAGACG302.1653686E-645.00000430
TGCGGTC302.1653686E-645.0000043
TGAGTAA302.1653686E-645.00000432
GCGTTAC302.1653686E-645.00000438
CCGTCAC302.1653686E-645.00000430
ATCGTCC302.1653686E-645.00000434
AAACGAT302.1653686E-645.00000438
AGACGAT453.8562575E-1045.00000420
ACTAGAC302.1653686E-645.00000427
CCCCGAA302.1653686E-645.00000417
Produced by FastQC (version 0.11.5)