##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545636_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1074745
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95477159698347	31.0	31.0	34.0	31.0	34.0
2	32.65427147835068	34.0	31.0	34.0	31.0	34.0
3	32.567855630870575	34.0	31.0	34.0	31.0	34.0
4	35.9920241545669	37.0	35.0	37.0	35.0	37.0
5	35.72940185811518	37.0	35.0	37.0	35.0	37.0
6	35.72794290738733	37.0	35.0	37.0	33.0	37.0
7	35.72720319703744	37.0	35.0	37.0	33.0	37.0
8	35.706591796193514	37.0	35.0	37.0	33.0	37.0
9	37.9319084992254	39.0	38.0	39.0	35.0	39.0
10	37.93388757333135	39.0	39.0	39.0	35.0	39.0
11	37.74953221461835	39.0	38.0	39.0	35.0	39.0
12	37.21353344281667	39.0	37.0	39.0	33.0	39.0
13	36.93780571205263	39.0	37.0	39.0	33.0	39.0
14	37.326393702692265	39.0	35.0	41.0	33.0	41.0
15	37.01909476201332	39.0	35.0	41.0	31.0	41.0
16	37.11723339024606	39.0	35.0	41.0	32.0	41.0
17	37.32622901246342	39.0	35.0	41.0	32.0	41.0
18	37.44109253823	39.0	35.0	41.0	33.0	41.0
19	37.46163136371884	39.0	35.0	41.0	33.0	41.0
20	37.40940595210957	39.0	35.0	41.0	33.0	41.0
21	37.38645446129082	39.0	35.0	41.0	33.0	41.0
22	37.30922218758868	39.0	35.0	41.0	33.0	41.0
23	37.216990076715874	39.0	35.0	41.0	32.0	41.0
24	37.20272157581566	39.0	35.0	41.0	32.0	41.0
25	37.16905963740236	39.0	35.0	41.0	32.0	41.0
26	37.091979027583285	39.0	35.0	41.0	32.0	41.0
27	36.99652103522231	39.0	35.0	41.0	32.0	41.0
28	36.89326956626921	38.0	35.0	41.0	32.0	41.0
29	36.821346458927465	38.0	35.0	41.0	32.0	41.0
30	36.75536522616993	38.0	35.0	41.0	31.0	41.0
31	36.72572470679091	38.0	35.0	41.0	31.0	41.0
32	36.628532349534076	38.0	35.0	41.0	31.0	41.0
33	36.467923088732675	38.0	35.0	41.0	31.0	41.0
34	36.42140228612369	38.0	35.0	41.0	31.0	41.0
35	36.29905605515727	38.0	35.0	41.0	30.0	41.0
36	36.26685958064471	38.0	35.0	41.0	30.0	41.0
37	36.1548395200722	38.0	35.0	41.0	30.0	41.0
38	36.04531679607721	38.0	35.0	40.0	30.0	41.0
39	35.99383202527111	38.0	35.0	40.0	30.0	41.0
40	35.89844939962503	38.0	35.0	40.0	30.0	41.0
41	35.8806023754472	38.0	35.0	40.0	30.0	41.0
42	35.733619602789496	38.0	35.0	40.0	29.0	41.0
43	35.66203890225123	38.0	35.0	40.0	29.0	41.0
44	35.63031509799999	37.0	35.0	40.0	29.0	41.0
45	35.62149067918436	37.0	35.0	40.0	29.0	41.0
46	35.54190994142797	37.0	34.0	40.0	29.0	41.0
47	35.40471460672067	37.0	34.0	40.0	28.0	41.0
48	35.38873686316289	37.0	34.0	40.0	28.0	41.0
49	35.36987471446715	37.0	34.0	40.0	28.0	41.0
50	35.28241117660468	37.0	34.0	40.0	28.0	41.0
51	35.190402374516744	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	1.0
11	1.0
12	9.0
13	5.0
14	2.0
15	13.0
16	34.0
17	98.0
18	186.0
19	475.0
20	783.0
21	1350.0
22	2149.0
23	3485.0
24	5168.0
25	7775.0
26	10735.0
27	13123.0
28	15327.0
29	18043.0
30	21741.0
31	27293.0
32	34043.0
33	44456.0
34	73123.0
35	169855.0
36	72318.0
37	94086.0
38	138138.0
39	320685.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.505101210054478	92.5634452823693	2.574192017641394	3.3572614899348214
2	3.159447124666782	1.9443681989681272	92.16074510697887	2.7354395693862266
3	26.021335293488224	15.758714857943046	45.515726986401425	12.704222862167306
4	27.392637323272034	14.637146485910613	46.02338229068291	11.94683390013445
5	23.3377219712583	14.11348738537979	48.64912141949951	13.899669223862404
6	20.471088490758273	14.541402844395648	50.05205886047388	14.9354498043722
7	21.520732824995697	16.349087457955143	48.85968299457081	13.270496722478356
8	22.086820594652686	18.22869610930965	46.34876179931054	13.33572149672713
9	81.99507790220005	3.3323253422904964	10.096906708102853	4.575690047406594
10	84.19867038227673	3.350376135734523	9.353939771759814	3.097013710228938
11	77.63041465649991	6.872886126476513	9.715700003256586	5.780999213766987
12	43.38238372823321	25.971369952872543	15.762064489716165	14.884181829178084
13	37.887684985740805	21.481281606334527	27.04697393335163	13.584059474573037
14	33.03192850397071	25.900748549655965	24.09297089076944	16.974352055603887
15	19.512721622338322	36.058599946964165	29.83572847512666	14.592949955570855
16	13.470823311576236	41.065648130486764	29.051356368254794	16.412172189682202
17	14.31148784130189	25.6300796933226	43.31929899650615	16.739133468869362
18	15.871671884958758	23.70022656537132	40.95008583431419	19.478015715355735
19	16.80556783236954	21.53738793853426	29.280433963405272	32.37661026569093
20	19.332027597243997	23.373544422165256	35.58974454405464	21.704683436536108
21	25.17322713760008	25.50977208547144	29.615118004736008	19.701882772192473
22	25.414307579937567	25.070784232538877	27.55007001660859	21.96483817091496
23	20.62135669391344	28.645120470437174	29.563477848233767	21.170044987415622
24	26.130105280787536	22.13213366891681	27.149230747758768	24.58853030253688
25	21.438108574592114	28.417996827154347	25.009560407352442	25.134334190901097
26	20.69151287049486	23.64067755607144	35.42924135492605	20.238568218507645
27	21.960092859236376	26.736946903684128	31.309473409971666	19.993486827107827
28	19.5219331097144	33.980246477071304	25.45301443598249	21.04480597723181
29	17.471214101949766	35.37992733159959	26.219149658756262	20.929708907694383
30	16.9492298173055	30.18250840897143	34.21016148016506	18.658100293558007
31	19.405533405598536	25.31400471739808	32.502965819799115	22.777496057204267
32	20.010886303262634	34.01988378638654	27.936394214441567	18.032835695909263
33	24.309022140135568	27.731787540300257	26.949741566604175	21.009448752960004
34	29.15203141210241	25.373646772024994	26.06497355186579	19.409348264006812
35	25.03924186667535	29.08857449906722	24.7380541430758	21.134129491181632
36	19.788787107639486	26.049341936924574	28.02060023540468	26.14127072003126
37	21.006750438476104	27.446417522295985	30.44340750596653	21.10342453326138
38	27.579565385277437	28.080428380685653	23.829838705925592	20.51016752811132
39	21.64532051789029	30.762832113664174	27.577983614717912	20.01386375372763
40	20.659877459304298	29.421490679184366	30.00562924228538	19.913002619225956
41	20.59939799673411	29.418513228719373	26.38328161563906	23.598807158907462
42	24.678505133775918	23.61341527525134	27.437671261555067	24.270408329417677
43	20.35785232776147	25.570716774676782	27.233715904702976	26.837714992858768
44	19.573573266216638	25.823893109528306	26.44478457680659	28.157749047448466
45	18.861962605083065	25.460737198126065	28.60008653215414	27.07721366463673
46	21.926038269543007	28.575894747126064	27.84418629535378	21.653880687977146
47	17.030179251822524	33.631977817994034	26.084233934561222	23.25360899562222
48	19.671456950253315	29.848475684929916	26.179698440095095	24.300368924721678
49	21.54306370348315	26.872979171803546	28.68820045685256	22.89575666786075
50	20.365109863269893	23.73530465366203	34.06966303634816	21.82992244671992
51	20.065503910229868	23.372427878240885	29.6291678491177	26.932900362411548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	813.0
1	905.0
2	997.0
3	3246.0
4	5495.0
5	3717.0
6	1939.0
7	2097.5
8	2256.0
9	2702.5
10	3149.0
11	3217.5
12	3286.0
13	3349.5
14	3413.0
15	3298.5
16	3184.0
17	3102.5
18	3021.0
19	3119.0
20	3217.0
21	3786.0
22	4355.0
23	5353.5
24	6352.0
25	6723.5
26	9497.5
27	11900.0
28	14428.5
29	16957.0
30	20481.5
31	24006.0
32	27010.0
33	30014.0
34	31338.0
35	32662.0
36	41210.5
37	49759.0
38	58755.0
39	67751.0
40	81505.5
41	95260.0
42	103221.5
43	111183.0
44	122650.5
45	134118.0
46	133407.0
47	132696.0
48	118114.0
49	103532.0
50	84278.5
51	65025.0
52	52795.0
53	40565.0
54	35816.0
55	31067.0
56	29381.5
57	27696.0
58	24355.5
59	21015.0
60	16774.5
61	12534.0
62	9611.5
63	6689.0
64	5380.0
65	4071.0
66	3980.0
67	3889.0
68	2743.0
69	1597.0
70	1215.5
71	834.0
72	791.5
73	749.0
74	597.5
75	235.0
76	24.0
77	63.5
78	103.0
79	57.0
80	11.0
81	8.5
82	6.0
83	6.5
84	7.0
85	3.5
86	0.0
87	3.5
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1074745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.013856331738328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.22930621053521	11.08628163601431
2	11.863020140775598	3.7994540038979716
3	5.439318270969585	2.6131338400171877
4	3.0517698485913307	1.954824156514896
5	2.009545254326905	1.609028449745881
6	1.313030798888993	1.2616011943533563
7	0.9323698037284396	1.0451585259470806
8	0.6803273197759367	0.871573116595876
9	0.5084912258821309	0.7328614893503351
>10	3.012683349670931	9.367505916865616
>50	0.5509607968851764	6.425576623300194
>100	1.352040304737273	50.72230044153102
>500	0.04664218386329532	4.452023999672289
>1k	0.009911464070950256	3.3328558995952875
>5k	5.830272982911915E-4	0.7258207065986992
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	7774	0.7233343723394852	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3805	0.3540374693531954	No Hit
ATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	3579	0.3330092254441751	No Hit
ATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	2845	0.2647139554033747	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	2637	0.24536052738091363	Illumina Single End Adapter 2 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	2626	0.2443370287835719	No Hit
ATATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	2404	0.22368096618267588	No Hit
ATGTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	2356	0.21921479048518486	No Hit
ATTGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	2234	0.20786326058739515	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	2063	0.19195250966508334	Illumina Single End Adapter 2 (95% over 21bp)
ATTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	1650	0.15352478960125426	No Hit
ATGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	1614	0.15017515782813598	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	1517	0.14114976110612285	No Hit
ATGGTCTGGGGTGTCCTCCAGCCTCCACATGTATGGCATGGTACACACACA	1410	0.13119391111379908	No Hit
ATTTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG	1409	0.13110086578676802	No Hit
ATTGGTCTGGGAGATGGAGAAACTAGGGGCCAGGGAGAGAAAGGAGCCACA	1330	0.12375028495131403	No Hit
ATTTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1130	0.10514121954510139	No Hit
ATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	1088	0.10123331580979675	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28564915398536395	0.0
2	0.0	0.0	0.0	1.3795830638895739	0.0
3	0.0	0.0	0.0	1.8914254078874524	0.0
4	0.0	0.0	0.0	2.4509069593252355	0.0
5	0.0	0.0	0.0	4.69516024731448	0.0
6	0.0	0.0	0.0	5.181135990397722	0.0
7	0.0	0.0	0.0	5.941502402895571	0.0
8	0.0	0.0	0.0	8.249770875882186	0.0
9	0.0	0.0	0.0	8.522579774737263	0.0
10	0.0	0.0	0.0	9.068662799082572	0.0
11	0.0	0.0	0.0	9.458243583361634	0.0
12	0.0	0.0	0.0	10.57711364091017	0.0
13	0.0	0.0	0.0	11.636713825139918	0.0
14	0.0	0.0	0.0	12.059418745842036	0.0
15	0.0	0.0	0.0	12.296591284444217	0.0
16	0.0	0.0	0.0	12.416340620333195	0.0
17	0.0	0.0	0.0	12.624110835593559	0.0
18	0.0	0.0	0.0	13.10980744269571	0.0
19	0.0	0.0	0.0	13.813974477666795	0.0
20	0.0	0.0	0.0	14.605976301355206	0.0
21	9.304532703106319E-5	0.0	0.0	15.05612959353149	0.0
22	1.8609065406212637E-4	0.0	0.0	15.533079939892719	0.0
23	1.8609065406212637E-4	0.0	0.0	16.241340969253173	0.0
24	1.8609065406212637E-4	0.0	0.0	16.843809461779305	0.0
25	1.8609065406212637E-4	0.0	0.0	17.47242369120117	0.0
26	2.7913598109318956E-4	0.0	0.0	17.923972663282917	0.0
27	2.7913598109318956E-4	0.0	0.0	18.346677583985038	0.0
28	2.7913598109318956E-4	0.0	0.0	18.728907787428646	0.0
29	3.7218130812425275E-4	0.0	0.0	19.09587855723916	0.0
30	3.7218130812425275E-4	0.0	0.0	19.495601282164607	0.0
31	3.7218130812425275E-4	0.0	0.0	19.911420848666427	0.0
32	3.7218130812425275E-4	0.0	0.0	20.430706818826792	0.0
33	3.7218130812425275E-4	0.0	0.0	20.86113450167249	0.0
34	3.7218130812425275E-4	0.0	0.0	21.2679286714523	0.0
35	3.7218130812425275E-4	0.0	0.0	21.642901339387482	0.0
36	3.7218130812425275E-4	0.0	0.0	22.045322378796833	0.0
37	3.7218130812425275E-4	0.0	0.0	22.51883004805791	0.0
38	3.7218130812425275E-4	0.0	0.0	22.936789657081448	0.0
39	3.7218130812425275E-4	0.0	0.0	23.393549167476937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCT	45	3.8562575E-10	45.000004	35
CTCCGTA	30	2.1653686E-6	45.000004	43
CCTAGTT	30	2.1653686E-6	45.000004	15
CCCTATG	45	3.8562575E-10	45.000004	25
CATGCCA	30	2.1653686E-6	45.000004	38
TCCGACA	60	0.0	45.000004	21
GCGCGAG	90	0.0	45.000004	3
GATCCTA	60	0.0	45.000004	28
CCGGAAT	60	0.0	45.000004	36
CGCGCCC	30	2.1653686E-6	45.000004	38
ATAGACG	30	2.1653686E-6	45.000004	30
TGCGGTC	30	2.1653686E-6	45.000004	3
TGAGTAA	30	2.1653686E-6	45.000004	32
GCGTTAC	30	2.1653686E-6	45.000004	38
CCGTCAC	30	2.1653686E-6	45.000004	30
ATCGTCC	30	2.1653686E-6	45.000004	34
AAACGAT	30	2.1653686E-6	45.000004	38
AGACGAT	45	3.8562575E-10	45.000004	20
ACTAGAC	30	2.1653686E-6	45.000004	27
CCCCGAA	30	2.1653686E-6	45.000004	17
>>END_MODULE