FastQCFastQC Report
Thu 26 May 2016
SRR1545635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545635_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences913716
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT62270.6815027864237903Illumina Single End Adapter 2 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC47570.5206212871395489Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC44040.4819878386719724Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT43660.47782899719387645Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC41230.45123430037342016Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC38630.4227790692074999Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC35150.38469283672388355Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC26430.2892583691212587Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC21550.23585008908676217Illumina Single End Adapter 2 (95% over 21bp)
GGTCTGGGGTGTCCTCCAGCCTCCACATGTATGGCATGGTACACACACACAC16600.1816757066747217No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC15050.16471201117196152Illumina Single End Adapter 2 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14750.1614287152682015Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT14740.1613192720714095Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14510.15880207854519346Illumina Single End Adapter 2 (95% over 21bp)
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCA12180.13330181369265723No Hit
ATGCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT10760.11776087974819309No Hit
TGGTCTGGGAGATGGAGAAACTAGGGGCCAGGGAGAGAAAGGAGCCACAGTC9920.10856765121766501No Hit
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT9890.10823932162728901Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC9380.10265771859089695Illumina Single End Adapter 2 (95% over 21bp)
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT9240.10112551383580895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT351.0192707E-746.00000433
GCCCCGA351.0192707E-746.00000410
ACAACGA351.0192707E-746.00000445
CGGCGTA351.0192707E-746.00000441
ACAGTCG351.0192707E-746.00000440
ACCGGGC351.0192707E-746.00000411
AGATCGA351.0192707E-746.00000429
TCCGCGC351.0192707E-746.00000412
CTCGTCA351.0192707E-746.00000430
GATAACG750.046.0000049
CAGCCGC351.0192707E-746.00000422
AGCCCGT351.0192707E-746.00000415
GGCACGG351.0192707E-746.0000042
CGGCCAC351.0192707E-746.00000434
CGTGAGC351.0192707E-746.00000419
CGTTGCG750.046.0000041
GTCTCCG351.0192707E-746.00000443
CCGGAAT351.0192707E-746.00000434
ACTTCGG351.0192707E-746.00000420
GTTAGTA351.0192707E-746.00000423
Produced by FastQC (version 0.11.5)