##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545635_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	913716
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57359179438688	33.0	31.0	34.0	31.0	34.0
2	32.94997242031441	34.0	31.0	34.0	31.0	34.0
3	32.989386198775115	34.0	33.0	34.0	31.0	34.0
4	36.37017738553336	37.0	37.0	37.0	35.0	37.0
5	36.35770195553104	37.0	37.0	37.0	35.0	37.0
6	36.37627118273074	37.0	37.0	37.0	35.0	37.0
7	36.48106742138695	37.0	37.0	37.0	35.0	37.0
8	36.54995315831177	37.0	37.0	37.0	35.0	37.0
9	38.366064510197916	39.0	39.0	39.0	37.0	39.0
10	38.100755595830655	39.0	39.0	39.0	35.0	39.0
11	37.91504800178611	39.0	38.0	39.0	35.0	39.0
12	37.37308638570409	39.0	35.0	39.0	35.0	39.0
13	37.21504603180857	39.0	35.0	39.0	35.0	39.0
14	38.47579225930158	40.0	36.0	41.0	35.0	41.0
15	38.57365527144102	40.0	36.0	41.0	35.0	41.0
16	38.67321136983483	40.0	36.0	41.0	35.0	41.0
17	38.66534130955352	40.0	36.0	41.0	35.0	41.0
18	38.67719619663003	40.0	36.0	41.0	35.0	41.0
19	38.66076439506367	40.0	36.0	41.0	35.0	41.0
20	38.571393080563325	40.0	36.0	41.0	35.0	41.0
21	38.5174058460178	40.0	36.0	41.0	35.0	41.0
22	38.60839144767083	40.0	36.0	41.0	35.0	41.0
23	38.62832543153453	40.0	36.0	41.0	35.0	41.0
24	38.590719654684825	40.0	36.0	41.0	35.0	41.0
25	38.513129900319136	40.0	35.0	41.0	35.0	41.0
26	38.38357104395676	40.0	35.0	41.0	35.0	41.0
27	38.342815491903394	40.0	35.0	41.0	35.0	41.0
28	38.3244268459784	40.0	36.0	41.0	35.0	41.0
29	38.29390970498492	40.0	36.0	41.0	35.0	41.0
30	38.27563925771246	40.0	36.0	41.0	35.0	41.0
31	38.16643793038537	40.0	35.0	41.0	35.0	41.0
32	38.157587259060804	40.0	35.0	41.0	35.0	41.0
33	38.07277972586668	40.0	35.0	41.0	35.0	41.0
34	38.0473560712519	40.0	35.0	41.0	34.0	41.0
35	38.026312333372736	40.0	35.0	41.0	34.0	41.0
36	37.92250983894339	40.0	35.0	41.0	34.0	41.0
37	37.83501000310819	40.0	35.0	41.0	34.0	41.0
38	37.70116425672747	40.0	35.0	41.0	34.0	41.0
39	37.65454911591786	40.0	35.0	41.0	34.0	41.0
40	37.52664941841885	40.0	35.0	41.0	33.0	41.0
41	37.423607554207216	39.0	35.0	41.0	33.0	41.0
42	37.444440066716574	39.0	35.0	41.0	34.0	41.0
43	37.407902455467564	39.0	35.0	41.0	33.0	41.0
44	37.282390808522564	39.0	35.0	41.0	33.0	41.0
45	37.09984502843334	39.0	35.0	41.0	33.0	41.0
46	37.13255431665857	39.0	35.0	41.0	33.0	41.0
47	37.1198589058307	39.0	35.0	41.0	33.0	41.0
48	37.05984463443784	39.0	35.0	41.0	33.0	41.0
49	37.07243279093285	39.0	35.0	41.0	33.0	41.0
50	36.95032373297611	39.0	35.0	41.0	33.0	41.0
51	36.81764683993713	38.0	35.0	41.0	33.0	41.0
52	36.442613459762114	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	3.0
13	2.0
14	4.0
15	3.0
16	13.0
17	28.0
18	52.0
19	134.0
20	248.0
21	401.0
22	741.0
23	1070.0
24	1712.0
25	2396.0
26	3170.0
27	3792.0
28	5155.0
29	6660.0
30	8545.0
31	10841.0
32	14629.0
33	20298.0
34	47913.0
35	140205.0
36	48215.0
37	60629.0
38	108344.0
39	427807.0
40	702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.70501556282258	14.172346768580171	46.938654899334146	12.183982769263096
2	25.468197995876178	12.926554859496825	50.54404213125304	11.061205013373959
3	24.262900069605873	13.761934780610169	46.55538482416856	15.419780325615399
4	21.16981644187034	14.300723638417189	49.8591466057287	14.670313313983776
5	22.127116084209973	16.096248724986758	47.882274142074785	13.89436104872849
6	21.459840913369142	17.96761794693318	47.0474414369454	13.525099702752277
7	83.49454316220795	2.5419276886910156	7.831536276042009	6.131992873059025
8	86.30252726230032	2.228810702669101	8.133490055991139	3.335171979039439
9	78.30113514483713	4.651007534069667	9.462896567423575	7.5849607536696295
10	42.55611152699526	25.367838584417918	14.59282753065504	17.483222357931787
11	36.76273590481068	19.6037937389736	29.547474269904434	14.085996086311283
12	32.136790862806386	26.630813075397608	23.755959182065325	17.476436879730684
13	19.803308686725416	33.48764824081005	32.11052449557631	14.598518576888223
14	13.299646717360755	41.66031896125273	28.827885250997028	16.212149070389486
15	13.933541713180025	25.349889900144028	41.95636280857509	18.760205578100855
16	15.901549277893787	24.99168231704381	38.77988346488406	20.32688494017835
17	16.31896563045848	23.108821559434222	27.095071116189274	33.47714169391802
18	18.673636009438383	24.69870287923162	33.20178261078935	23.42587850054065
19	23.133774608302797	26.724277565457978	29.791423155553804	20.35052467068542
20	24.581817545057763	23.780036685359565	26.516444934749966	25.121700834832705
21	19.862845785780266	30.059668430891	29.687671004994986	20.389814778333747
22	26.09268087677134	21.630462857167874	26.586816910287226	25.690039355773564
23	21.53251119603903	29.022913027680374	23.19407780973519	26.250497966545407
24	21.168831453099212	21.48205788231792	34.01013006229507	23.3389806022878
25	24.245498601315944	25.764898502379296	28.23185760126779	21.75774529503697
26	17.84219604340955	34.20209342946824	24.307224564306633	23.64848596281558
27	17.843509361771055	34.43072026756673	24.387008654768003	23.338761715894215
28	18.032079989843673	30.896689999956223	31.12531683805471	19.945913172145392
29	19.590879441752143	25.215384211286658	30.4824475000985	24.7112888468627
30	18.9935384736614	36.39478787719598	26.31857163495003	18.293102014192595
31	23.62605010747322	29.07019248869452	26.449903471100427	20.853853932731834
32	31.31060417022357	25.81447627052607	23.149972201428014	19.72494735782234
33	25.69189989011903	29.698943654264564	23.86233796934715	20.746818486269255
34	18.522166625078253	25.824107271843765	27.60288754930416	28.050838553773822
35	22.195080309417804	27.24030223833226	28.762109889725036	21.802507562524898
36	28.750837240455457	26.06335010003108	22.720298210822616	22.46551444869084
37	23.1830240468592	29.47513231682492	25.356347048754756	21.985496587561123
38	19.473446891594325	27.874306677348322	31.970437203682543	20.68180922737481
39	21.51806469406249	28.755105525130347	25.738084919165257	23.988744861641912
40	26.349325173248577	22.410902293491635	28.26206392358238	22.977708609677407
41	21.302352153185453	24.675829251102094	26.707970529135967	27.313848066576483
42	19.9567480486278	26.384894212205978	24.456723971124507	29.201633768041713
43	19.601167102250592	24.248781897219704	28.614142687662248	27.53590831286746
44	25.852234173419316	26.906062715329487	24.47215546187218	22.769547649379017
45	17.588944486032858	31.425519526855172	25.877843881468642	25.10769210564333
46	20.327213269768723	28.48116920355997	25.266384740991732	25.925232785679576
47	23.125456925346608	25.472356837354276	28.91270372851083	22.48948250878829
48	19.933217761317522	22.39382915479208	35.09646323365247	22.57648985023793
49	20.514908352267007	21.24686445241191	28.546506792044795	29.69172040327629
50	18.683157567559284	25.383270075165587	31.755381321986263	24.178191035288865
51	22.237763156166686	25.881674393356356	27.246540500549404	24.634021949927547
52	20.830433088618346	22.033761037346398	30.289061371367033	26.846744502668223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	459.0
1	406.5
2	354.0
3	275.0
4	196.0
5	231.5
6	267.0
7	314.5
8	362.0
9	637.0
10	912.0
11	968.5
12	1025.0
13	1007.5
14	1089.0
15	1188.0
16	1229.0
17	1270.0
18	1150.5
19	1031.0
20	1535.5
21	2040.0
22	2201.0
23	2362.0
24	2870.5
25	3379.0
26	5175.5
27	6972.0
28	7086.0
29	7200.0
30	9484.5
31	11769.0
32	13982.0
33	16195.0
34	18099.5
35	20004.0
36	22424.5
37	24845.0
38	28185.5
39	41979.0
40	52432.0
41	63470.0
42	74508.0
43	80245.5
44	85983.0
45	92663.0
46	99343.0
47	111582.5
48	123822.0
49	119294.0
50	114766.0
51	94610.5
52	74455.0
53	59673.0
54	44891.0
55	36959.5
56	29028.0
57	27194.0
58	25360.0
59	23055.5
60	20751.0
61	16912.5
62	13074.0
63	10669.5
64	6472.5
65	4680.0
66	3936.0
67	3192.0
68	2597.5
69	2003.0
70	1777.5
71	1552.0
72	1085.0
73	618.0
74	506.5
75	395.0
76	257.5
77	120.0
78	99.5
79	79.0
80	53.5
81	28.0
82	19.5
83	11.0
84	8.5
85	6.0
86	4.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	913716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.960279715914808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.87900509815542	10.195267893409085
2	12.660943624725268	4.041444034360872
3	6.203414311912298	2.970246828354884
4	3.75796619905725	2.399127667996276
5	2.411282798312368	1.9242373967619593
6	1.7575453337883538	1.683054908439779
7	1.234061515356898	1.3787176880219254
8	0.9851717126785594	1.257889288204533
9	0.707049991527464	1.0156244074122167
>10	4.229562748942954	12.93915408048776
>50	0.703170268911896	8.116324750794401
>100	1.4406415265512016	45.00986461061361
>500	0.019208553687349354	2.042110578014601
>1k	0.01029029661822287	4.345137434373999
>5k	6.860197745481913E-4	0.6817984327541109
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	6227	0.6815027864237903	Illumina Single End Adapter 2 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	4757	0.5206212871395489	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	4404	0.4819878386719724	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	4366	0.47782899719387645	Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	4123	0.45123430037342016	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3863	0.4227790692074999	Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3515	0.38469283672388355	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	2643	0.2892583691212587	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	2155	0.23585008908676217	Illumina Single End Adapter 2 (95% over 21bp)
GGTCTGGGGTGTCCTCCAGCCTCCACATGTATGGCATGGTACACACACACAC	1660	0.1816757066747217	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1505	0.16471201117196152	Illumina Single End Adapter 2 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1475	0.1614287152682015	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1474	0.1613192720714095	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1451	0.15880207854519346	Illumina Single End Adapter 2 (95% over 21bp)
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCA	1218	0.13330181369265723	No Hit
ATGCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1076	0.11776087974819309	No Hit
TGGTCTGGGAGATGGAGAAACTAGGGGCCAGGGAGAGAAAGGAGCCACAGTC	992	0.10856765121766501	No Hit
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	989	0.10823932162728901	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	938	0.10265771859089695	Illumina Single End Adapter 2 (95% over 21bp)
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	924	0.10112551383580895	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7455270565471109	0.0
2	0.0	0.0	0.0	1.5975423435728389	0.0
3	0.0	0.0	0.0	5.589811276151452	0.0
4	0.0	0.0	0.0	6.399253159625092	0.0
5	0.0	0.0	0.0	7.70742769087988	0.0
6	0.0	0.0	0.0	11.66511257327222	0.0
7	0.0	0.0	0.0	12.136922194642537	0.0
8	0.0	0.0	0.0	13.101773417560818	0.0
9	0.0	0.0	0.0	13.679305167032206	0.0
10	0.0	0.0	0.0	15.63308511616301	0.0
11	0.0	0.0	0.0	17.399498312385905	0.0
12	0.0	0.0	0.0	18.11667958096389	0.0
13	0.0	0.0	0.0	18.453436297492875	0.0
14	0.0	0.0	0.0	18.63467423138043	0.0
15	0.0	0.0	0.0	18.935971352148808	0.0
16	0.0	0.0	0.0	19.697039342640384	0.0
17	1.0944319679200102E-4	0.0	0.0	20.834263600506066	0.0
18	1.0944319679200102E-4	0.0	0.0	22.096143659517836	0.0
19	2.1888639358400204E-4	0.0	0.0	22.773706490857116	0.0
20	2.1888639358400204E-4	0.0	0.0	23.474252393522715	0.0
21	2.1888639358400204E-4	0.0	0.0	24.57557928284062	0.0
22	2.1888639358400204E-4	0.0	0.0	25.47038685981202	0.0
23	2.1888639358400204E-4	0.0	0.0	26.405797862793253	0.0
24	5.47215983960005E-4	0.0	0.0	27.062894816332427	0.0
25	5.47215983960005E-4	0.0	0.0	27.663300193933345	0.0
26	5.47215983960005E-4	0.0	0.0	28.21927163363671	0.0
27	5.47215983960005E-4	0.0	0.0	28.744161205451146	0.0
28	5.47215983960005E-4	0.0	0.0	29.30658979376524	0.0
29	5.47215983960005E-4	0.0	0.0	29.8763510762644	0.0
30	5.47215983960005E-4	0.0	0.0	30.539905178414298	0.0
31	5.47215983960005E-4	0.0	0.0	31.121048553379826	0.0
32	5.47215983960005E-4	0.0	0.0	31.66005629758043	0.0
33	5.47215983960005E-4	0.0	0.0	32.146202977730496	0.0
34	5.47215983960005E-4	0.0	0.0	32.6715303223321	0.0
35	5.47215983960005E-4	0.0	0.0	33.27521899583678	0.0
36	5.47215983960005E-4	0.0	0.0	33.79222865748219	0.0
37	5.47215983960005E-4	0.0	0.0	34.37293425966055	0.0
38	5.47215983960005E-4	0.0	0.0	34.88490953425353	0.0
39	5.47215983960005E-4	0.0	0.0	35.4558746919174	0.0
40	5.47215983960005E-4	0.0	0.0	35.994882436118004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCT	35	1.0192707E-7	46.000004	33
GCCCCGA	35	1.0192707E-7	46.000004	10
ACAACGA	35	1.0192707E-7	46.000004	45
CGGCGTA	35	1.0192707E-7	46.000004	41
ACAGTCG	35	1.0192707E-7	46.000004	40
ACCGGGC	35	1.0192707E-7	46.000004	11
AGATCGA	35	1.0192707E-7	46.000004	29
TCCGCGC	35	1.0192707E-7	46.000004	12
CTCGTCA	35	1.0192707E-7	46.000004	30
GATAACG	75	0.0	46.000004	9
CAGCCGC	35	1.0192707E-7	46.000004	22
AGCCCGT	35	1.0192707E-7	46.000004	15
GGCACGG	35	1.0192707E-7	46.000004	2
CGGCCAC	35	1.0192707E-7	46.000004	34
CGTGAGC	35	1.0192707E-7	46.000004	19
CGTTGCG	75	0.0	46.000004	1
GTCTCCG	35	1.0192707E-7	46.000004	43
CCGGAAT	35	1.0192707E-7	46.000004	34
ACTTCGG	35	1.0192707E-7	46.000004	20
GTTAGTA	35	1.0192707E-7	46.000004	23
>>END_MODULE