SRR1545634_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545634_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1328358
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	11198	0.842995638224033	No Hit
ATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	5100	0.3839326446635621	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4246	0.31964274691009503	No Hit
ATGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	3824	0.28787420258695323	No Hit
ATTGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	3764	0.2833573479438525	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3492	0.26288094022846253	No Hit
ATGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	3426	0.2579124001210517	No Hit
ATATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2933	0.22079891113690736	No Hit
AACTGCTTTTGGAATAATAGAAAGTTTTGTGAAATGTTGACCTAGTGTATA	2864	0.21560452829734153	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2802	0.21093711183280411	No Hit
ATGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2724	0.20506520079677315	No Hit
ATTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2587	0.19475171602835983	No Hit
ATGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2148	0.16170339622300614	No Hit
ATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2063	0.1553045188119468	No Hit
ATAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1840	0.13851687572175572	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1777	0.13377417834649996	No Hit
ATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	1695	0.12760114366759562	No Hit
ATTCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1490	0.1121685569703348	No Hit
ATCTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1471	0.11073821966668623	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTA	125	0.0	45.000004	2
TCGAGTA	125	0.0	45.000004	2
CGGACAG	35	1.21212E-7	45.0	8
TGATCCG	45	3.8562575E-10	45.0	3
CCTTCGC	35	1.21212E-7	45.0	12
AGCCGGT	20	7.033684E-4	45.0	14
GGTACGA	20	7.033684E-4	45.0	44
TCCATCG	40	6.8157533E-9	45.0	17
ACCGGTA	45	3.8562575E-10	45.0	21
CGAACTA	40	6.8157533E-9	45.0	24
ACCGGAC	35	1.21212E-7	45.0	40
CGTATTA	75	0.0	45.0	32
CACGGTC	20	7.033684E-4	45.0	41
ACGTTCC	40	6.8157533E-9	45.0	22
ACGTTCA	35	1.21212E-7	45.0	34
CGTGCCG	70	0.0	45.0	37
GCGAAAG	20	7.033684E-4	45.0	13
TCCGAAT	40	6.8157533E-9	45.0	31
CGGCCCC	20	7.033684E-4	45.0	30
CGTTGTA	20	7.033684E-4	45.0	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content