##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545634_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1328358
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945886575757438	31.0	31.0	34.0	31.0	34.0
2	32.647826113141186	34.0	31.0	34.0	31.0	34.0
3	32.55862952607656	34.0	31.0	34.0	31.0	34.0
4	35.99190805490689	37.0	35.0	37.0	35.0	37.0
5	35.73254499163629	37.0	35.0	37.0	35.0	37.0
6	35.73484482345874	37.0	35.0	37.0	33.0	37.0
7	35.70595351554325	37.0	35.0	37.0	33.0	37.0
8	35.670713768426886	37.0	35.0	37.0	33.0	37.0
9	37.94150372113542	39.0	38.0	39.0	35.0	39.0
10	37.9529878240655	39.0	39.0	39.0	35.0	39.0
11	37.72264028221308	39.0	38.0	39.0	35.0	39.0
12	37.19465610927175	39.0	37.0	39.0	33.0	39.0
13	36.86696658581497	39.0	37.0	39.0	32.0	39.0
14	37.320030443600295	39.0	35.0	41.0	33.0	41.0
15	37.0549136603235	39.0	35.0	41.0	31.0	41.0
16	37.165218261944446	39.0	35.0	41.0	32.0	41.0
17	37.35573617955401	39.0	35.0	41.0	32.0	41.0
18	37.47028286049393	39.0	35.0	41.0	33.0	41.0
19	37.46367921900572	39.0	35.0	41.0	33.0	41.0
20	37.42368548237749	39.0	35.0	41.0	33.0	41.0
21	37.419934987405505	39.0	35.0	41.0	33.0	41.0
22	37.307858273146245	39.0	35.0	41.0	32.0	41.0
23	37.249555466222205	39.0	35.0	41.0	32.0	41.0
24	37.21504744955803	39.0	35.0	41.0	32.0	41.0
25	37.16972457726005	39.0	35.0	41.0	32.0	41.0
26	37.09483889132297	39.0	35.0	41.0	32.0	41.0
27	37.041178658162934	39.0	35.0	41.0	32.0	41.0
28	36.96546488220796	39.0	35.0	41.0	32.0	41.0
29	36.90988649144282	39.0	35.0	41.0	32.0	41.0
30	36.851096617026435	38.0	35.0	41.0	31.0	41.0
31	36.8263081187451	38.0	35.0	41.0	31.0	41.0
32	36.7588504002686	38.0	35.0	41.0	31.0	41.0
33	36.63753972950063	38.0	35.0	41.0	31.0	41.0
34	36.53421743234881	38.0	35.0	41.0	31.0	41.0
35	36.452783812797456	38.0	35.0	41.0	31.0	41.0
36	36.39945029878994	38.0	35.0	41.0	30.0	41.0
37	36.27398111051388	38.0	35.0	41.0	30.0	41.0
38	36.167800397182084	38.0	35.0	40.0	30.0	41.0
39	36.092969666309834	38.0	35.0	40.0	30.0	41.0
40	36.01162412542402	38.0	35.0	40.0	30.0	41.0
41	36.02898239781746	38.0	35.0	40.0	30.0	41.0
42	35.91998391999747	38.0	35.0	40.0	30.0	41.0
43	35.82827746736949	38.0	35.0	40.0	30.0	41.0
44	35.76127670402106	38.0	35.0	40.0	29.0	41.0
45	35.73633011582721	38.0	35.0	40.0	29.0	41.0
46	35.66220250866107	38.0	34.0	40.0	29.0	41.0
47	35.55244971611569	37.0	34.0	40.0	28.0	41.0
48	35.536563185526795	37.0	34.0	40.0	29.0	41.0
49	35.51919587942407	37.0	34.0	40.0	29.0	41.0
50	35.41984841435818	37.0	34.0	40.0	28.0	41.0
51	35.34002957034173	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	4.0
12	4.0
13	2.0
14	8.0
15	11.0
16	44.0
17	113.0
18	249.0
19	526.0
20	927.0
21	1521.0
22	2680.0
23	3968.0
24	6310.0
25	9536.0
26	13099.0
27	15696.0
28	18408.0
29	22082.0
30	26936.0
31	33534.0
32	42064.0
33	54496.0
34	87717.0
35	198217.0
36	89317.0
37	117377.0
38	176939.0
39	406326.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.4006013439148182	92.90161236654576	2.4181734140946944	3.2796128754447222
2	3.30927355426775	2.0379295340563313	92.32782126505053	2.324975646625383
3	25.058681469904947	14.369017990632043	46.81531635297111	13.756984186491895
4	25.761353490549986	15.072066415830673	46.74545566782449	12.421124425794854
5	22.500636123695568	14.637770841896536	49.18124481502728	13.680348219380619
6	20.76480888435196	13.837007794585496	51.340527177161576	14.057656143900966
7	21.65651127181076	16.923901538591256	47.7475951513071	13.671992038290881
8	22.611524905183693	19.059620975670715	45.55804986306402	12.770804256081568
9	82.24379271250672	2.9655409159277846	10.180237556441863	4.610428815123633
10	84.74424816201656	2.723964473432614	9.380076756416567	3.151710608134253
11	77.64887176499107	6.32690886041263	9.867897057871447	6.156322316724859
12	42.55095388441971	28.76566407549772	14.634307920003492	14.049074120079075
13	34.48061441268092	23.1454171239982	29.011230406260964	13.36273805705992
14	31.355026280565934	26.883867150271236	24.568753302949958	17.192353266212873
15	18.903864771394456	35.04484483851492	31.735872407890042	14.315417982200582
16	14.448891036904207	40.5467501983652	28.013984181975037	16.990374582755553
17	12.979934626057133	26.56294462787893	42.85629325829332	17.600827487770616
18	16.213475584142227	23.364108169634992	42.85433595461464	17.568080291608133
19	16.491412706514357	22.79641482190795	29.08861918247942	31.623553289098272
20	18.713178224544887	23.989767818615164	35.672010105709454	21.625043851130492
21	24.087482440727577	25.74516809474554	29.767351873516024	20.39999759101086
22	23.700689121456715	25.11868035574747	27.97845159211598	23.202178930679832
23	20.60318076903967	28.922925897988343	31.078218371854575	19.39567496111741
24	27.345866099349724	21.622258457433915	26.338682794848978	24.693192648367383
25	21.477944951586846	28.93451915823897	24.21019032519848	25.377345564975705
26	20.417914447761824	23.467845264604872	36.01423712583505	20.10000316179825
27	21.57844496739584	25.976205209740144	31.059172301442832	21.386177521421182
28	17.90548933344776	33.568887302971035	26.112764781783227	22.412858581797977
29	18.301090519272666	33.22214342820234	26.2460119937547	22.2307540587703
30	17.23285439617934	27.470606568410023	33.57250078668552	21.724038248725115
31	16.649050933558573	27.039021107261746	31.411110559051096	24.90081740012858
32	19.18744796207047	33.641006415439215	26.358255831635745	20.813289790854576
33	22.76404403029906	28.17922578100181	24.659316238544125	24.397413950155002
34	25.173259016018275	29.255065276077687	25.422363549585274	20.149312158318764
35	24.372420687796513	29.665120396760514	23.94166331666614	22.020795598776836
36	18.970563658290914	28.41395166062161	25.846420919661718	26.76906376142576
37	20.02351775650841	27.218264955682127	30.848159908699312	21.91005737911015
38	24.203716166876703	29.498297898608662	25.227912957199788	21.07007297731485
39	22.158107979927095	31.71140611190658	27.251388556398197	18.879097351768124
40	19.676246915364683	29.10375064553381	28.908321401308985	22.31168103779252
41	20.06672899926074	29.73490580099642	25.92132542582647	24.27703977391637
42	24.29337573154225	25.763536636960822	25.723863597012254	24.21922403448468
43	19.050662547295232	26.494965965500267	28.43043818006893	26.02393330713558
44	19.014904114704017	28.816478690232607	25.104151139978832	27.064466055084544
45	19.746333443243465	25.449464677443885	27.136509886642006	27.66769199267065
46	21.708455100206418	27.137187414838472	27.94442462047129	23.20993286448382
47	17.315512836148088	31.324838635367875	26.308720992383076	25.050927536100957
48	19.28817382061161	28.65507641765247	26.52741203801987	25.529337723716043
49	20.235433520180553	27.005822225634958	29.240912464862635	23.517831789321857
50	20.01132224897204	25.952567003774586	32.35603655038777	21.680074196865604
51	19.618355895022276	24.294655507024462	28.59756180186365	27.48942679608961
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1049.0
1	1049.0
2	1049.0
3	3541.0
4	6033.0
5	4062.0
6	2091.0
7	2364.0
8	2637.0
9	2914.0
10	3191.0
11	3480.0
12	3769.0
13	3704.0
14	3639.0
15	3522.5
16	3406.0
17	3337.0
18	3268.0
19	3761.0
20	4254.0
21	5455.5
22	6657.0
23	6346.5
24	6036.0
25	6591.0
26	12167.0
27	17188.0
28	22504.5
29	27821.0
30	29997.5
31	32174.0
32	33483.5
33	34793.0
34	39320.5
35	43848.0
36	52544.0
37	61240.0
38	72427.0
39	83614.0
40	98183.0
41	112752.0
42	127322.5
43	141893.0
44	152885.0
45	163877.0
46	166980.0
47	170083.0
48	150894.0
49	131705.0
50	107647.0
51	83589.0
52	67122.5
53	50656.0
54	42979.0
55	35302.0
56	31751.5
57	28201.0
58	24743.0
59	21285.0
60	17970.5
61	14656.0
62	11248.5
63	7841.0
64	6115.0
65	4389.0
66	4025.5
67	3662.0
68	2593.5
69	1525.0
70	1183.0
71	841.0
72	672.0
73	503.0
74	455.0
75	220.5
76	34.0
77	129.5
78	225.0
79	121.5
80	18.0
81	10.5
82	3.0
83	3.0
84	3.0
85	2.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1328358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.680009017518916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.6201247556643	11.279042906902347
2	12.19029612064019	4.066684984370084
3	5.683619315729832	2.8440846431555484
4	3.3214248222074714	2.2160558394172716
5	2.117944255350986	1.7663664638928416
6	1.4603070064015042	1.4614760421073885
7	1.0044848664390436	1.1728371641115172
8	0.7607516414937455	1.0151475392166394
9	0.5451016450569444	0.8183070319512804
>10	3.387311753725091	10.906583982573647
>50	0.5995589872924288	7.260697831621492
>100	1.2733275394454653	47.5439170706571
>500	0.025792348880025413	2.7543675119531645
>1k	0.009049946975447514	3.6643133844247986
>5k	4.524973487723757E-4	0.38493065275425803
>10k+	4.524973487723757E-4	0.8451869508906239
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	11198	0.842995638224033	No Hit
ATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	5100	0.3839326446635621	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4246	0.31964274691009503	No Hit
ATGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	3824	0.28787420258695323	No Hit
ATTGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	3764	0.2833573479438525	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3492	0.26288094022846253	No Hit
ATGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	3426	0.2579124001210517	No Hit
ATATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2933	0.22079891113690736	No Hit
AACTGCTTTTGGAATAATAGAAAGTTTTGTGAAATGTTGACCTAGTGTATA	2864	0.21560452829734153	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2802	0.21093711183280411	No Hit
ATGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2724	0.20506520079677315	No Hit
ATTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2587	0.19475171602835983	No Hit
ATGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2148	0.16170339622300614	No Hit
ATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	2063	0.1553045188119468	No Hit
ATAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1840	0.13851687572175572	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1777	0.13377417834649996	No Hit
ATCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	1695	0.12760114366759562	No Hit
ATTCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1490	0.1121685569703348	No Hit
ATCTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	1471	0.11073821966668623	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2873472362119248	0.0
2	0.0	0.0	0.0	1.4578148360607608	0.0
3	0.0	0.0	0.0	2.059760998164651	0.0
4	0.0	0.0	0.0	2.688130759930681	0.0
5	7.52809107183455E-5	0.0	0.0	5.113681703275773	0.0
6	7.52809107183455E-5	0.0	0.0	5.60842784851674	0.0
7	1.50561821436691E-4	0.0	0.0	6.3707223504507065	0.0
8	1.50561821436691E-4	0.0	0.0	8.75660025384723	0.0
9	1.50561821436691E-4	0.0	0.0	9.071951988846381	0.0
10	1.50561821436691E-4	0.0	0.0	9.689330737647532	0.0
11	1.50561821436691E-4	0.0	0.0	10.1258094579925	0.0
12	1.50561821436691E-4	0.0	0.0	11.364029877487846	0.0
13	1.50561821436691E-4	0.0	0.0	12.512440170496207	0.0
14	1.50561821436691E-4	0.0	0.0	13.00372339384413	0.0
15	1.50561821436691E-4	0.0	0.0	13.252978489232571	0.0
16	1.50561821436691E-4	0.0	0.0	13.385096487543267	0.0
17	1.50561821436691E-4	0.0	0.0	13.607777421448134	0.0
18	1.50561821436691E-4	0.0	0.0	14.142271887548386	0.0
19	2.2584273215503652E-4	0.0	0.0	14.869786608730477	0.0
20	2.2584273215503652E-4	0.0	0.0	15.71278224695451	0.0
21	4.5168546431007304E-4	0.0	0.0	16.218594686071075	0.0
22	4.5168546431007304E-4	0.0	0.0	16.73931274550987	0.0
23	4.5168546431007304E-4	0.0	0.0	17.548657816642802	0.0
24	5.269663750284185E-4	0.0	0.0	18.200590503463676	0.0
25	6.02247285746764E-4	0.0	0.0	18.957314218004484	0.0
26	7.52809107183455E-4	0.0	0.0	19.50483228165901	0.0
27	7.52809107183455E-4	0.0	0.0	19.994760448614002	0.0
28	7.52809107183455E-4	0.0	0.0	20.475052658997047	0.0
29	7.52809107183455E-4	0.0	0.0	20.907692052895378	0.0
30	7.52809107183455E-4	0.0	0.0	21.369164035598835	0.0
31	8.280900179018006E-4	0.0	0.0	21.82446298362339	0.0
32	9.033709286201461E-4	0.0	0.0	22.37326082276013	0.0
33	9.033709286201461E-4	0.0	0.0	22.919574391843163	0.0
34	9.033709286201461E-4	0.0	0.0	23.385939633743313	0.0
35	9.033709286201461E-4	0.0	0.0	23.846282402785995	0.0
36	9.033709286201461E-4	0.0	0.0	24.319498207561516	0.0
37	9.033709286201461E-4	0.0	0.0	24.8889982971458	0.0
38	9.033709286201461E-4	0.0	0.0	25.37169949667183	0.0
39	9.033709286201461E-4	0.0	0.0	25.89414901705715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTA	125	0.0	45.000004	2
TCGAGTA	125	0.0	45.000004	2
CGGACAG	35	1.21212E-7	45.0	8
TGATCCG	45	3.8562575E-10	45.0	3
CCTTCGC	35	1.21212E-7	45.0	12
AGCCGGT	20	7.033684E-4	45.0	14
GGTACGA	20	7.033684E-4	45.0	44
TCCATCG	40	6.8157533E-9	45.0	17
ACCGGTA	45	3.8562575E-10	45.0	21
CGAACTA	40	6.8157533E-9	45.0	24
ACCGGAC	35	1.21212E-7	45.0	40
CGTATTA	75	0.0	45.0	32
CACGGTC	20	7.033684E-4	45.0	41
ACGTTCC	40	6.8157533E-9	45.0	22
ACGTTCA	35	1.21212E-7	45.0	34
CGTGCCG	70	0.0	45.0	37
GCGAAAG	20	7.033684E-4	45.0	13
TCCGAAT	40	6.8157533E-9	45.0	31
CGGCCCC	20	7.033684E-4	45.0	30
CGTTGTA	20	7.033684E-4	45.0	18
>>END_MODULE