FastQCFastQC Report
Thu 26 May 2016
SRR1545633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545633_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1146460
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT86320.7529263995254959Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC66040.576034052649024No Hit
TTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC63810.5565828724944613No Hit
TGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC63780.5563211974251173No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT55380.48305217800882716No Hit
ATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC43460.37907995045618686No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC40550.3536974687298292No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC35290.3078171065715332No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC27260.23777541301048447No Hit
CTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC27080.23620536259442107No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC24400.21282905639969993No Hit
TCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC23940.20881670533642693No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT22850.19930917781693214No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT14250.12429565793834936No Hit
TACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC12730.11103745442492544No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC12280.10711232838476702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT351.01956175E-746.00000444
AAGTACG351.01956175E-746.00000417
GCGACTT351.01956175E-746.0000049
TCCGCCG551.8189894E-1246.00000416
CGTATAA351.01956175E-746.00000426
CTCCGGT551.8189894E-1246.00000421
CGACGTC351.01956175E-746.00000446
GACGTTC351.01956175E-746.0000049
TAGCGTA351.01956175E-746.00000418
GATCCGC351.01956175E-746.0000049
GCCGGAT551.8189894E-1246.00000419
GCTGCGA351.01956175E-746.00000413
ATTCGGC700.046.00000410
ATTCGAC351.01956175E-746.00000444
TACGGTA351.01956175E-746.00000420
ATGTCGC750.046.00000432
TACATTA1500.046.00000422
AGACGTT351.01956175E-746.0000048
TCAATCG351.01956175E-746.00000433
TCGTGCA351.01956175E-746.00000413
Produced by FastQC (version 0.11.5)