##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545633_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1146460
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.571067459832875	33.0	31.0	34.0	31.0	34.0
2	32.933916577987894	34.0	31.0	34.0	31.0	34.0
3	32.98293442422762	34.0	33.0	34.0	31.0	34.0
4	36.381605114875356	37.0	37.0	37.0	35.0	37.0
5	36.34287109886084	37.0	37.0	37.0	35.0	37.0
6	36.3478743261867	37.0	37.0	37.0	35.0	37.0
7	36.47678069884689	37.0	37.0	37.0	35.0	37.0
8	36.54690525617989	37.0	37.0	37.0	35.0	37.0
9	38.341620292029376	39.0	39.0	39.0	37.0	39.0
10	38.08184323918846	39.0	39.0	39.0	35.0	39.0
11	37.8704211224116	39.0	38.0	39.0	35.0	39.0
12	37.394290249986916	39.0	37.0	39.0	35.0	39.0
13	37.24281614709628	39.0	37.0	39.0	35.0	39.0
14	38.550358494845	40.0	37.0	41.0	35.0	41.0
15	38.64422134221866	40.0	37.0	41.0	35.0	41.0
16	38.73595328227762	41.0	37.0	41.0	35.0	41.0
17	38.71319452924655	40.0	37.0	41.0	35.0	41.0
18	38.727313643738114	40.0	37.0	41.0	35.0	41.0
19	38.7193569771296	40.0	37.0	41.0	35.0	41.0
20	38.607572876506815	40.0	36.0	41.0	35.0	41.0
21	38.57590147061389	40.0	36.0	41.0	35.0	41.0
22	38.65037419534916	40.0	37.0	41.0	35.0	41.0
23	38.65356139769377	40.0	37.0	41.0	35.0	41.0
24	38.630593304607224	40.0	36.0	41.0	35.0	41.0
25	38.581947036965964	40.0	36.0	41.0	35.0	41.0
26	38.489793800045355	40.0	36.0	41.0	35.0	41.0
27	38.44537184027354	40.0	36.0	41.0	35.0	41.0
28	38.4386999982555	40.0	36.0	41.0	35.0	41.0
29	38.416426216352946	40.0	36.0	41.0	35.0	41.0
30	38.410692915583624	40.0	36.0	41.0	35.0	41.0
31	38.35063063691712	40.0	36.0	41.0	35.0	41.0
32	38.2920101878827	40.0	35.0	41.0	35.0	41.0
33	38.2370924410795	40.0	36.0	41.0	35.0	41.0
34	38.16918165483314	40.0	35.0	41.0	35.0	41.0
35	38.13785042653036	40.0	35.0	41.0	34.0	41.0
36	38.04214713116899	40.0	35.0	41.0	34.0	41.0
37	37.956811402055024	40.0	35.0	41.0	34.0	41.0
38	37.84974617518274	40.0	35.0	41.0	34.0	41.0
39	37.83947804546168	40.0	35.0	41.0	34.0	41.0
40	37.75925980845385	40.0	35.0	41.0	34.0	41.0
41	37.656400572196155	40.0	35.0	41.0	34.0	41.0
42	37.653564886694696	40.0	35.0	41.0	34.0	41.0
43	37.56968319871605	40.0	35.0	41.0	33.0	41.0
44	37.474924550355006	40.0	35.0	41.0	33.0	41.0
45	37.352485913158766	39.0	35.0	41.0	33.0	41.0
46	37.397578633358336	39.0	35.0	41.0	33.0	41.0
47	37.39679796940146	39.0	35.0	41.0	33.0	41.0
48	37.313564363344554	39.0	35.0	41.0	33.0	41.0
49	37.326748425588335	39.0	35.0	41.0	33.0	41.0
50	37.20841808698079	39.0	35.0	41.0	33.0	41.0
51	37.08525373759224	39.0	35.0	41.0	33.0	41.0
52	36.726715280079546	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	7.0
13	11.0
14	13.0
15	12.0
16	25.0
17	47.0
18	77.0
19	173.0
20	339.0
21	497.0
22	809.0
23	1286.0
24	2126.0
25	2930.0
26	3738.0
27	4736.0
28	6073.0
29	8250.0
30	10654.0
31	13907.0
32	18215.0
33	25423.0
34	56758.0
35	158207.0
36	57097.0
37	73761.0
38	136021.0
39	564309.0
40	953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.347417267065573	13.070844163773703	48.34961533764807	13.232123231512658
2	24.116846640964358	12.972367112677286	51.182945763480625	11.72784048287773
3	23.25192331175968	14.648221481778693	46.806343003680894	15.293512202780734
4	21.06527920729899	13.736283865115222	51.499659822409846	13.698777105175933
5	22.50518988887532	16.88100762346702	46.498002546970675	14.115799940686985
6	21.72845105803953	18.8714826509429	46.180852362925876	13.219213928091692
7	83.65647296896533	1.974687298292134	7.980653489873175	6.388186242869355
8	86.6722781431537	1.727840482877728	7.888282190394781	3.7115991835737834
9	77.73999965109991	4.050293948327896	9.941210334420738	8.268496066151458
10	41.00247719065646	28.270415016659978	13.738551715716204	16.98855607696736
11	32.87319226139595	21.01006576766743	31.862167018474256	14.254574952462363
12	30.433508364879717	26.969802696997714	24.543551454041136	18.053137484081432
13	19.077682605585892	31.834952811262497	34.80112694729864	14.286237635852975
14	14.152521675418244	40.864923329204686	28.16836173961586	16.81419325576121
15	12.691153638155713	25.82532316871064	41.41731940058964	20.066203792544005
16	16.013729218638243	25.148893114456676	40.38387732672749	18.45350034017759
17	16.177188911955064	23.81609476126511	27.501003087765817	32.50571323901401
18	17.786490588420005	25.0	33.700434380615114	23.513075030964885
19	22.00887950735307	26.619158104076895	30.522303438410407	20.849658950159622
20	22.921689374247684	23.971180852362924	26.91676988294402	26.190359890445368
21	19.66549203635539	29.952898487518098	31.748861713448356	18.632747762678157
22	26.744936587408198	21.009978542644316	26.37510248940216	25.86998238054533
23	21.393245294210004	29.507178619402335	22.634719048200548	26.464857038187116
24	20.583012054498194	21.731416708825428	34.503166268339065	23.182404968337316
25	24.488076339340232	25.32657048654118	28.387470997679813	21.797882176438776
26	17.109711634073584	32.6893219126703	25.368700172705545	24.832266280550563
27	16.858503567503448	33.207613000017446	24.838720932261047	25.09516250021806
28	18.0117928231251	26.81279765539138	31.56499136472271	23.61041815676081
29	15.546464769813165	27.656438078956093	29.477522111543358	27.31957503968739
30	21.160703382586394	32.59834621356175	24.352528653420094	21.888421750431764
31	21.485703818711514	26.94974094168135	22.288871831551035	29.275683408056103
32	26.077665160581265	30.67442387872233	22.168588524675958	21.079322436020444
33	24.8420354831394	30.281649599637145	22.365280951799452	22.511033965424
34	17.03164523838599	30.53905064284842	24.000488460129443	28.428815658636147
35	20.8435531985416	26.95924846920084	29.01985241526089	23.177345916996668
36	22.725433072239763	29.645081380946564	23.39532124976013	24.23416429705354
37	23.588088550843466	32.11982973675488	24.68843221743454	19.603649494967115
38	18.64522094098355	27.56807913054097	30.556408422448232	23.23029150602725
39	20.67974460513232	29.898033947979	24.64682588140886	24.775395565479823
40	25.39399542940879	25.534863841738918	26.627008356157216	22.444132372695076
41	19.122254592397468	27.2946286830766	27.398252010536783	26.184864713989146
42	18.876977827399124	29.944699335345327	23.213282626519895	27.965040210735655
43	19.350260802819115	24.62597910088446	27.23924079339881	28.784519302897614
44	25.44441149276905	25.59609580796539	24.286063185806743	24.67342951345882
45	17.793119690176717	28.577708773092823	26.21330007152452	27.41587146520594
46	19.765539137867872	27.254592397466986	25.0327966086911	27.94707185597404
47	21.218708022957625	25.87617535718647	29.115974390733214	23.78914222912269
48	18.941175444411492	24.805313748408143	33.92678331559758	22.326727491582783
49	19.725502852258256	22.531095720740364	27.084939727509028	30.65846169949235
50	18.736632765207684	24.848839034942344	30.743767772098458	25.670760427751517
51	21.916682657920905	26.20667096976781	26.030040297960678	25.84660607435061
52	19.759433386249846	23.00376812099855	28.883606929155835	28.353191563595765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	597.0
1	472.0
2	347.0
3	265.5
4	184.0
5	203.5
6	223.0
7	386.5
8	550.0
9	660.5
10	771.0
11	931.5
12	1092.0
13	1136.0
14	1237.0
15	1294.0
16	1433.0
17	1572.0
18	1454.0
19	1336.0
20	1739.0
21	2142.0
22	3370.0
23	4598.0
24	3859.5
25	3121.0
26	5069.0
27	7017.0
28	9072.5
29	11128.0
30	13223.0
31	15318.0
32	19321.5
33	23325.0
34	24253.5
35	25182.0
36	27612.0
37	30042.0
38	36282.0
39	52153.0
40	61784.0
41	75170.5
42	88557.0
43	97828.5
44	107100.0
45	117150.5
46	127201.0
47	144147.0
48	161093.0
49	155212.0
50	149331.0
51	123580.5
52	97830.0
53	78356.5
54	58883.0
55	48555.5
56	38228.0
57	32142.0
58	26056.0
59	23314.0
60	20572.0
61	16646.5
62	12721.0
63	11338.5
64	7692.5
65	5429.0
66	4598.0
67	3767.0
68	2660.5
69	1554.0
70	1261.0
71	968.0
72	759.0
73	550.0
74	586.5
75	623.0
76	507.5
77	392.0
78	213.5
79	35.0
80	104.5
81	174.0
82	117.5
83	61.0
84	34.5
85	8.0
86	12.0
87	16.0
88	11.5
89	6.5
90	6.0
91	4.5
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	4.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1146460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.55283349747807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.65803889168862	10.371680850526271
2	12.7409158927496	4.217965187561125
3	6.394978869144576	3.1756506132252227
4	3.899072016846479	2.5816275955814314
5	2.605401050113629	2.1563384888342707
6	1.8002198575116208	1.7879243736146133
7	1.298738013434428	1.5048455865257866
8	1.040171555443589	1.3774229252856407
9	0.7630402937630049	1.1367431041073197
>10	4.700318523323604	14.887114716033647
>50	0.7879555084836358	9.42519498834601
>100	1.2868885145185733	40.211231941320044
>500	0.014767573117426252	1.5731793304007535
>1k	0.00685637323309076	2.6655873748682692
>5k	0.0026370666281118308	2.9274929237696155
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	8632	0.7529263995254959	Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	6604	0.576034052649024	No Hit
TTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	6381	0.5565828724944613	No Hit
TGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	6378	0.5563211974251173	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	5538	0.48305217800882716	No Hit
ATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	4346	0.37907995045618686	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	4055	0.3536974687298292	No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	3529	0.3078171065715332	No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2726	0.23777541301048447	No Hit
CTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2708	0.23620536259442107	No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2440	0.21282905639969993	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2394	0.20881670533642693	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2285	0.19930917781693214	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	1425	0.12429565793834936	No Hit
TACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1273	0.11103745442492544	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1228	0.10711232838476702	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7804895068297193	0.0
2	0.0	0.0	0.0	1.663293965772901	0.0
3	0.0	0.0	0.0	5.910105891178061	0.0
4	0.0	0.0	0.0	6.748076688240323	0.0
5	0.0	0.0	0.0	8.055667009751758	0.0
6	0.0	0.0	0.0	12.103256982363101	0.0
7	0.0	0.0	0.0	12.640475899726113	0.0
8	0.0	0.0	0.0	13.721193936116393	0.0
9	0.0	0.0	0.0	14.383580761648902	0.0
10	0.0	0.0	0.0	16.523210578650804	0.0
11	0.0	0.0	0.0	18.415906355215185	0.0
12	0.0	0.0	0.0	19.229018020689775	0.0
13	8.722502311463112E-5	0.0	0.0	19.59658426809483	0.0
14	8.722502311463112E-5	0.0	0.0	19.78568811820735	0.0
15	8.722502311463112E-5	0.0	0.0	20.110426879263123	0.0
16	8.722502311463112E-5	0.0	0.0	20.93435444760393	0.0
17	8.722502311463112E-5	0.0	0.0	22.096017305444587	0.0
18	8.722502311463112E-5	0.0	0.0	23.421750431763865	0.0
19	4.3612511557315564E-4	0.0	0.0	24.20555448947194	0.0
20	4.3612511557315564E-4	0.0	0.0	24.99171362280411	0.0
21	4.3612511557315564E-4	0.0	0.0	26.21129389599288	0.0
22	5.233501386877867E-4	0.0	0.0	27.17818327721857	0.0
23	5.233501386877867E-4	0.0	0.0	28.246951485442143	0.0
24	7.850252080316802E-4	0.0	0.0	29.048026097726915	0.0
25	7.850252080316802E-4	0.0	0.0	29.767806988468852	0.0
26	9.594752542609424E-4	0.0	0.0	30.415540010118104	0.0
27	9.594752542609424E-4	0.0	0.0	31.00858294227448	0.0
28	9.594752542609424E-4	0.0	0.0	31.612877902412645	0.0
29	9.594752542609424E-4	0.0	0.0	32.24979502119568	0.0
30	9.594752542609424E-4	0.0	0.0	32.96477853566631	0.0
31	9.594752542609424E-4	0.0	0.0	33.66510824625369	0.0
32	9.594752542609424E-4	0.0	0.0	34.28911606161576	0.0
33	9.594752542609424E-4	0.0	0.0	34.89768504788654	0.0
34	9.594752542609424E-4	0.0	0.0	35.5192505626014	0.0
35	9.594752542609424E-4	0.0	0.0	36.2441777297071	0.0
36	9.594752542609424E-4	0.0	0.0	36.82684088411283	0.0
37	9.594752542609424E-4	0.0	0.0	37.488093784344855	0.0
38	9.594752542609424E-4	0.0	0.0	38.09456937006088	0.0
39	0.0011339253004902046	0.0	0.0	38.706801807302476	0.0
40	0.0011339253004902046	0.0	0.0	39.31676639394309	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	35	1.01956175E-7	46.000004	44
AAGTACG	35	1.01956175E-7	46.000004	17
GCGACTT	35	1.01956175E-7	46.000004	9
TCCGCCG	55	1.8189894E-12	46.000004	16
CGTATAA	35	1.01956175E-7	46.000004	26
CTCCGGT	55	1.8189894E-12	46.000004	21
CGACGTC	35	1.01956175E-7	46.000004	46
GACGTTC	35	1.01956175E-7	46.000004	9
TAGCGTA	35	1.01956175E-7	46.000004	18
GATCCGC	35	1.01956175E-7	46.000004	9
GCCGGAT	55	1.8189894E-12	46.000004	19
GCTGCGA	35	1.01956175E-7	46.000004	13
ATTCGGC	70	0.0	46.000004	10
ATTCGAC	35	1.01956175E-7	46.000004	44
TACGGTA	35	1.01956175E-7	46.000004	20
ATGTCGC	75	0.0	46.000004	32
TACATTA	150	0.0	46.000004	22
AGACGTT	35	1.01956175E-7	46.000004	8
TCAATCG	35	1.01956175E-7	46.000004	33
TCGTGCA	35	1.01956175E-7	46.000004	13
>>END_MODULE