FastQCFastQC Report
Thu 26 May 2016
SRR1545632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545632_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2666852
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC94400.35397539870978967No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65120.24418302927946509No Hit
ATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT49500.18561209995905284No Hit
ATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG49220.18456217292898142No Hit
ATGCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC39160.1468397946342729No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT38320.1436900135440587No Hit
ATTGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC35020.1313158735467885No Hit
ATGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC34500.12936600906237017No Hit
ATATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC31320.11744183779227343No Hit
ATGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC28460.10671758312797261No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC28360.1063426091886614No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT28120.10544267173431447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA302.1665837E-645.00000417
ACAATCG453.8562575E-1045.035
CGTAATC351.2127384E-745.032
CTCGACC207.034964E-445.043
ACGTAAC207.034964E-445.012
GCCCGTA406.8212103E-945.040
AATCGAC207.034964E-445.038
AAGTCGA207.034964E-445.037
ATTCGAT1350.040.0000041
CACTACG451.9299478E-840.09
TGCGAAC451.9299478E-840.014
CGACTAT403.4605182E-739.37525
CCGTTGA403.4605182E-739.37530
TAAGACG659.094947E-1238.0769273
CGTTTTT35000.037.992861
ATTTGTT39650.037.906681
ATCGTAG3950.037.5949361
GGTCGAA301.1403259E-437.50000421
CTATACG601.5643309E-1037.5000043
ATTACGA601.5643309E-1037.5000041