##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545632_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2666852
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911982742199417	31.0	31.0	34.0	31.0	34.0
2	32.58882945135313	34.0	31.0	34.0	31.0	34.0
3	32.56254790292075	34.0	31.0	34.0	31.0	34.0
4	36.02572208731493	37.0	35.0	37.0	35.0	37.0
5	35.80332991857066	37.0	35.0	37.0	35.0	37.0
6	35.80570912821559	37.0	35.0	37.0	35.0	37.0
7	35.77922696872567	37.0	35.0	37.0	35.0	37.0
8	35.749153683818975	37.0	35.0	37.0	33.0	37.0
9	37.93615993688439	39.0	38.0	39.0	35.0	39.0
10	37.87930863804966	39.0	38.0	39.0	35.0	39.0
11	37.70909784269993	39.0	38.0	39.0	35.0	39.0
12	37.22750343851102	39.0	37.0	39.0	33.0	39.0
13	36.99955978059525	39.0	37.0	39.0	33.0	39.0
14	37.721073760373656	40.0	36.0	41.0	33.0	41.0
15	37.593897599116865	40.0	36.0	41.0	33.0	41.0
16	37.42139646294582	40.0	36.0	41.0	32.0	41.0
17	37.58073526389916	40.0	36.0	41.0	32.0	41.0
18	37.672191782671106	40.0	36.0	41.0	33.0	41.0
19	37.67023329378608	40.0	35.0	41.0	33.0	41.0
20	37.63090940179657	40.0	35.0	41.0	33.0	41.0
21	37.61862863031019	40.0	35.0	41.0	33.0	41.0
22	37.5429866374287	40.0	35.0	41.0	32.0	41.0
23	37.48033449175282	40.0	35.0	41.0	32.0	41.0
24	37.50358850059921	40.0	35.0	41.0	32.0	41.0
25	37.45182372325123	39.0	35.0	41.0	32.0	41.0
26	37.377761120602116	39.0	35.0	41.0	32.0	41.0
27	37.282622357746135	39.0	35.0	41.0	32.0	41.0
28	37.20499525282993	39.0	35.0	41.0	32.0	41.0
29	37.16693914772923	39.0	35.0	41.0	32.0	41.0
30	37.092578065824426	39.0	35.0	41.0	32.0	41.0
31	36.98032511740434	39.0	35.0	41.0	31.0	41.0
32	36.86686062818634	39.0	35.0	41.0	31.0	41.0
33	36.73448882802645	39.0	35.0	41.0	31.0	41.0
34	36.703980198376215	39.0	35.0	41.0	31.0	41.0
35	36.61708936228932	39.0	35.0	41.0	30.0	41.0
36	36.46982884689514	39.0	35.0	41.0	30.0	41.0
37	36.37002390833837	39.0	35.0	41.0	30.0	41.0
38	36.30707928298983	38.0	35.0	41.0	30.0	41.0
39	36.246299007219	38.0	35.0	41.0	30.0	41.0
40	36.16331239978822	38.0	35.0	41.0	30.0	41.0
41	36.07498691340952	38.0	35.0	40.0	30.0	41.0
42	35.92940515634164	38.0	35.0	40.0	29.0	41.0
43	35.79100302528974	38.0	35.0	40.0	29.0	41.0
44	35.75580384663266	38.0	35.0	40.0	29.0	41.0
45	35.73353226950727	38.0	35.0	40.0	29.0	41.0
46	35.680758062314666	37.0	34.0	40.0	29.0	41.0
47	35.61309214009626	37.0	34.0	40.0	29.0	41.0
48	35.559431869485074	37.0	34.0	40.0	29.0	41.0
49	35.51129908971326	37.0	34.0	40.0	29.0	41.0
50	35.40576642423352	37.0	34.0	40.0	29.0	41.0
51	35.21814408898582	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	20.0
11	23.0
12	29.0
13	46.0
14	52.0
15	83.0
16	197.0
17	412.0
18	797.0
19	1367.0
20	2381.0
21	3853.0
22	5910.0
23	8920.0
24	12652.0
25	17790.0
26	24213.0
27	29657.0
28	36004.0
29	43134.0
30	52315.0
31	65632.0
32	82235.0
33	108735.0
34	181255.0
35	280399.0
36	196716.0
37	272554.0
38	416398.0
39	822667.0
40	394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.012897603616549	91.54711247568294	3.3011580695141687	3.1388318511863424
2	3.045650827267505	2.9566695114689527	91.27701874719708	2.7206609140664724
3	28.564764748849953	15.556093851477323	42.42117672821739	13.457964671455333
4	27.236644553203554	15.788202719910965	43.38950942909468	13.585643297790803
5	24.962015139947773	14.25170950618932	45.86669976436637	14.919575589496532
6	23.33361581370095	14.36870137525442	47.15428527717324	15.143397533871397
7	22.586142763077966	17.283261313338723	44.69239387862544	15.438202044957874
8	26.28132344802036	19.094947901120875	42.93567847034631	11.688050180512455
9	82.40986001472898	3.9159278430149103	9.141077195134937	4.533134947121175
10	83.33435826210078	3.9787359778495395	8.838585718292578	3.848320041757098
11	76.78528842245463	6.402942495496563	10.236338574469075	6.575430507579723
12	42.841934985518506	22.29486300702101	16.909599782815093	17.953602224645387
13	34.8855129568495	24.13785991873565	23.28385677195435	17.692770352460503
14	30.276295797442078	23.173014475493954	26.82312329293114	19.72756643413283
15	24.558618176036763	24.52232069871144	31.64198838180746	19.277072743444332
16	19.50606932818169	29.229706035430535	31.554094490432917	19.710130145954857
17	19.304595830589776	23.28389426934828	37.740602028159046	19.670907871902905
18	24.834074031854787	21.630746663106915	33.07495129088529	20.460228014153017
19	24.432139466307092	22.024469299383693	28.34394259598958	25.199448638319637
20	25.29349210229889	22.19823222286051	30.64804496087522	21.86023071396538
21	27.048820107002562	24.75236721047887	26.858783314559638	21.340029367958927
22	26.477172336522614	24.52123327428744	26.711906022531434	22.289688366658517
23	26.447211918771647	23.583460949464012	29.401856570968317	20.567470560796025
24	28.47004633177994	20.727284453730466	27.94013316074533	22.862536053744265
25	27.24020680562701	23.34640242503146	26.407764660356104	23.005626108985425
26	26.79702510675508	22.451189642319857	28.669494970099578	22.082290280825482
27	28.489807458381645	22.058966901800325	26.201266511977416	23.249959127840615
28	22.812852006785526	25.366237046525264	26.630274195943382	25.19063675074582
29	21.969123145941357	23.80904527135364	29.760706630889157	24.461124951815847
30	22.761143100554513	24.09308803038189	28.893504401444098	24.2522644676195
31	23.003226275773834	24.632150565535696	28.195415418628407	24.169207740062067
32	24.0945504287452	24.50233458774615	28.649396366952494	22.75371861655615
33	24.212592224840375	24.316197524272063	28.16575498002889	23.305455270858673
34	28.18375372911583	23.382662404962858	26.27779869299084	22.155785172930482
35	26.31994576376942	24.67583502946545	24.750454843388383	24.253764363376746
36	23.145453890954578	24.431652000185988	28.61099903556703	23.811895073292405
37	23.63854462114883	25.5589361539373	29.13450015223942	21.66801907267445
38	25.391772771792358	27.531149085138583	25.149502109603382	21.927576033465673
39	24.187318981330797	28.344467559504615	25.745485688744633	21.722727770419954
40	25.280218024847272	24.72686898260571	26.885106485099286	23.10780650744773
41	23.77124789827107	25.81560581539583	24.362319318807344	26.050826967525758
42	25.55649882333178	23.072071491031373	27.950557436258176	23.420872249378668
43	22.451302134501653	24.275512851856796	27.598381912457082	25.67480310118447
44	21.22551232689328	25.650354800341375	27.930271346141446	25.193861526623902
45	21.620472377169786	23.082945735271398	28.876930553326545	26.41965133423227
46	24.67947227667677	22.937905815545818	27.486827165511997	24.895794742265412
47	21.030713365421104	23.80162078735528	27.855239060885268	27.31242678633835
48	21.48053210301884	24.866209298453757	27.077468115965942	26.575790482561462
49	22.803027689575575	24.59585308821037	29.984716062233673	22.61640315998038
50	22.028556515322183	23.557212773712227	30.60829772330823	23.805932987657357
51	22.476800362374817	23.52526499408291	28.197627764870344	25.80030687867193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	938.0
1	1578.0
2	2218.0
3	6405.5
4	10593.0
5	7182.0
6	3771.0
7	3852.5
8	3934.0
9	4111.0
10	4288.0
11	4639.0
12	4990.0
13	5105.0
14	5220.0
15	5102.0
16	4984.0
17	4903.0
18	4822.0
19	5759.5
20	6697.0
21	7527.0
22	8357.0
23	10794.5
24	13232.0
25	15422.5
26	21115.0
27	24617.0
28	30397.0
29	36177.0
30	42984.5
31	49792.0
32	54340.5
33	58889.0
34	64742.0
35	70595.0
36	79930.5
37	89266.0
38	102839.5
39	116413.0
40	133643.0
41	150873.0
42	177742.0
43	204611.0
44	224417.5
45	244224.0
46	239727.0
47	235230.0
48	218053.0
49	200876.0
50	184904.0
51	168932.0
52	167998.0
53	167064.0
54	169057.5
55	171051.0
56	165100.5
57	159150.0
58	145329.0
59	131508.0
60	115716.0
61	99924.0
62	84666.5
63	69409.0
64	57870.0
65	46331.0
66	38219.5
67	30108.0
68	24759.5
69	19411.0
70	16594.5
71	13778.0
72	10771.0
73	7764.0
74	6198.0
75	3648.5
76	2665.0
77	1867.0
78	1069.0
79	713.5
80	358.0
81	208.0
82	58.0
83	176.0
84	294.0
85	171.0
86	48.0
87	41.5
88	35.0
89	37.5
90	40.0
91	21.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2666852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.891072945321799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.15369267630174	11.307085206481204
2	11.253719795253119	3.576673643451584
3	4.674617182896816	2.22854047934604
4	2.5734752819485824	1.6358113371371004
5	1.5839974456368522	1.258570947690931
6	1.0290516546997708	0.9811640945602896
7	0.7582456058713193	0.8434535363359599
8	0.5423475907368274	0.6894788100894766
9	0.4235135605414837	0.605707639550791
>10	3.0555664750678826	10.48945749632194
>50	1.1093808881795937	13.117234673746253
>100	1.8300427356950744	49.91590130867885
>500	0.006649519245919541	0.7203915585592486
>1k	0.0052246222646510675	2.0285236028957376
>5k	4.7496566042282433E-4	0.602005665154651
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	9440	0.35397539870978967	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6512	0.24418302927946509	No Hit
ATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	4950	0.18561209995905284	No Hit
ATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	4922	0.18456217292898142	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3916	0.1468397946342729	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	3832	0.1436900135440587	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3502	0.1313158735467885	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3450	0.12936600906237017	No Hit
ATATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3132	0.11744183779227343	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	2846	0.10671758312797261	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2836	0.1063426091886614	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2812	0.10544267173431447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.749739393112179E-5	0.0	0.0	0.15655161966243347	0.0
2	3.749739393112179E-5	0.0	0.0	0.6656162396713429	0.0
3	3.749739393112179E-5	0.0	0.0	0.936309926460111	0.0
4	3.749739393112179E-5	0.0	0.0	1.280498505353878	0.0
5	3.749739393112179E-5	0.0	0.0	2.5163001171418586	0.0
6	3.749739393112179E-5	0.0	0.0	2.8001928865943815	0.0
7	3.749739393112179E-5	0.0	0.0	3.2675229071579523	0.0
8	3.749739393112179E-5	0.0	0.0	4.669625461030458	0.0
9	7.499478786224358E-5	0.0	0.0	4.85988723783697	0.0
10	7.499478786224358E-5	0.0	0.0	5.208987975335714	0.0
11	7.499478786224358E-5	0.0	0.0	5.469482370975217	0.0
12	7.499478786224358E-5	0.0	0.0	6.239491355350803	0.0
13	7.499478786224358E-5	0.0	0.0	6.918831641200937	0.0
14	1.4998957572448716E-4	0.0	0.0	7.190762741989432	0.0
15	1.4998957572448716E-4	0.0	0.0	7.348664267833386	0.0
16	1.4998957572448716E-4	0.0	0.0	7.464381225504828	0.0
17	1.4998957572448716E-4	0.0	0.0	7.627194909953758	0.0
18	1.4998957572448716E-4	0.0	0.0	7.944572852186773	0.0
19	1.4998957572448716E-4	0.0	0.0	8.339382912887555	0.0
20	1.4998957572448716E-4	0.0	0.0	8.749566905100096	0.0
21	1.4998957572448716E-4	0.0	0.0	9.056858048365639	0.0
22	1.4998957572448716E-4	0.0	0.0	9.409746022651426	0.0
23	1.8748696965560893E-4	0.0	0.0	9.907898901026378	0.0
24	1.8748696965560893E-4	0.0	0.0	10.366267044440411	0.0
25	2.2498436358673072E-4	0.0	0.0	10.819798024037329	0.0
26	2.624817575178525E-4	0.0	0.0	11.173960909716774	0.0
27	2.999791514489743E-4	0.0	0.0	11.496738476675871	0.0
28	3.374765453800961E-4	0.0	0.0	11.790155584186898	0.0
29	3.7497393931121785E-4	0.0	0.0	12.123844892779951	0.0
30	3.7497393931121785E-4	0.0	0.0	12.427048820107002	0.0
31	3.7497393931121785E-4	0.0	0.0	12.785448911300664	0.0
32	3.7497393931121785E-4	0.0	0.0	13.171259597457977	0.0
33	3.7497393931121785E-4	0.0	0.0	13.522310199441138	0.0
34	3.7497393931121785E-4	0.0	0.0	13.857911875124678	0.0
35	9.749322422091664E-4	0.0	0.0	14.218524312560277	0.0
36	0.001087424424002532	0.0	0.0	14.59271080659894	0.0
37	0.0011249218179336536	0.0	0.0	15.014481493536199	0.0
38	0.0011999166057958973	0.0	0.0	15.377156287638009	0.0
39	0.0011999166057958973	0.0	0.0	15.77230382488417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	30	2.1665837E-6	45.000004	17
ACAATCG	45	3.8562575E-10	45.0	35
CGTAATC	35	1.2127384E-7	45.0	32
CTCGACC	20	7.034964E-4	45.0	43
ACGTAAC	20	7.034964E-4	45.0	12
GCCCGTA	40	6.8212103E-9	45.0	40
AATCGAC	20	7.034964E-4	45.0	38
AAGTCGA	20	7.034964E-4	45.0	37
ATTCGAT	135	0.0	40.000004	1
CACTACG	45	1.9299478E-8	40.0	9
TGCGAAC	45	1.9299478E-8	40.0	14
CGACTAT	40	3.4605182E-7	39.375	25
CCGTTGA	40	3.4605182E-7	39.375	30
TAAGACG	65	9.094947E-12	38.076927	3
CGTTTTT	3500	0.0	37.99286	1
ATTTGTT	3965	0.0	37.90668	1
ATCGTAG	395	0.0	37.594936	1
GGTCGAA	30	1.1403259E-4	37.500004	21
CTATACG	60	1.5643309E-10	37.500004	3
ATTACGA	60	1.5643309E-10	37.500004	1
>>END_MODULE