FastQCFastQC Report
Thu 26 May 2016
SRR1545631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545631_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2154411
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT81210.3769475740701287Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT71640.33252708048742785No Hit
GTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC69410.3221762235710828No Hit
TGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC60210.27947313674131813No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC59200.27478508046978967No Hit
TTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC49490.2297147573048968No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC44830.20808471549764646No Hit
ATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC43990.20418573800449402No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC32780.1521529550304004No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC27010.12537069296434153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC351.02012564E-746.00000446
ATCGTAC351.02012564E-746.00000412
CGGAATT850.046.014
CTAGACG301.8621668E-646.025
AGCGTAT206.31269E-446.034
CGTGATA206.31269E-446.020
CGACACG206.31269E-446.018
GTTCGAC253.4181576E-546.018
ACAATCG206.31269E-446.033
ACGTATA253.4181576E-546.017
CGATGCG253.4181576E-546.01
ACCCGTT206.31269E-446.026
CAATCGT206.31269E-446.09
ATCATCG253.4181576E-546.014
CCGTTGA206.31269E-446.028
TACCGGA301.8621668E-646.011
TTTAGCG1350.044.29631
ATTAGCG554.7293724E-1141.818181
TTTACGC451.589433E-840.8888925
TATTCCG451.589433E-840.8888913
Produced by FastQC (version 0.11.5)