##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545631_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2154411
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.580776834132394	33.0	31.0	34.0	31.0	34.0
2	32.981465467823924	34.0	31.0	34.0	31.0	34.0
3	33.05795458712381	34.0	33.0	34.0	31.0	34.0
4	36.42904255501852	37.0	37.0	37.0	35.0	37.0
5	36.398673697822744	37.0	37.0	37.0	35.0	37.0
6	36.41130916988448	37.0	37.0	37.0	35.0	37.0
7	36.49244874817293	37.0	37.0	37.0	35.0	37.0
8	36.53200294651299	37.0	37.0	37.0	35.0	37.0
9	38.346837256215274	39.0	39.0	39.0	37.0	39.0
10	38.11356236112794	39.0	39.0	39.0	35.0	39.0
11	37.94520590546558	39.0	38.0	39.0	35.0	39.0
12	37.545350446131216	39.0	37.0	39.0	35.0	39.0
13	37.430519524826046	39.0	37.0	39.0	35.0	39.0
14	38.48636866410355	41.0	38.0	41.0	34.0	41.0
15	38.664136508772	41.0	38.0	41.0	34.0	41.0
16	38.79241379662469	41.0	38.0	41.0	35.0	41.0
17	38.77096895624837	41.0	38.0	41.0	35.0	41.0
18	38.75214292908827	40.0	38.0	41.0	35.0	41.0
19	38.73514013807022	40.0	38.0	41.0	35.0	41.0
20	38.61873477252019	40.0	38.0	41.0	34.0	41.0
21	38.594503091564235	40.0	37.0	41.0	35.0	41.0
22	38.74867608826728	40.0	38.0	41.0	35.0	41.0
23	38.77777360030189	40.0	38.0	41.0	35.0	41.0
24	38.73539867741113	40.0	38.0	41.0	35.0	41.0
25	38.66044408425319	40.0	37.0	41.0	35.0	41.0
26	38.56157669079855	40.0	37.0	41.0	35.0	41.0
27	38.507275074254636	40.0	37.0	41.0	35.0	41.0
28	38.454700611907384	40.0	37.0	41.0	35.0	41.0
29	38.381669514312726	40.0	36.0	41.0	35.0	41.0
30	38.32722354276876	40.0	36.0	41.0	34.0	41.0
31	38.23986973701861	40.0	36.0	41.0	34.0	41.0
32	38.216620691223724	40.0	36.0	41.0	34.0	41.0
33	38.1416507806542	40.0	36.0	41.0	34.0	41.0
34	37.98057195214841	40.0	35.0	41.0	34.0	41.0
35	37.928956452598875	40.0	35.0	41.0	34.0	41.0
36	37.86984702547471	40.0	35.0	41.0	34.0	41.0
37	37.79142884064368	40.0	35.0	41.0	34.0	41.0
38	37.703784004073505	40.0	35.0	41.0	34.0	41.0
39	37.589788577945434	40.0	35.0	41.0	33.0	41.0
40	37.454737745026364	40.0	35.0	41.0	33.0	41.0
41	37.28387851714459	39.0	35.0	41.0	33.0	41.0
42	37.27539127863718	39.0	35.0	41.0	33.0	41.0
43	37.21884867836267	39.0	35.0	41.0	33.0	41.0
44	37.12236708780265	39.0	35.0	41.0	33.0	41.0
45	37.06196217898999	39.0	35.0	41.0	33.0	41.0
46	37.05884253283148	39.0	35.0	41.0	33.0	41.0
47	37.020074628285876	39.0	35.0	41.0	33.0	41.0
48	36.92679762589404	39.0	35.0	41.0	33.0	41.0
49	36.845406934888466	38.0	35.0	41.0	33.0	41.0
50	36.75065017770518	38.0	35.0	41.0	33.0	41.0
51	36.62122779729587	38.0	35.0	40.0	32.0	41.0
52	36.30925436232919	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	6.0
12	12.0
13	20.0
14	32.0
15	64.0
16	117.0
17	191.0
18	326.0
19	654.0
20	983.0
21	1552.0
22	2249.0
23	3279.0
24	4555.0
25	6058.0
26	7872.0
27	9773.0
28	12526.0
29	16342.0
30	20343.0
31	27221.0
32	37022.0
33	53306.0
34	139979.0
35	208933.0
36	121692.0
37	183674.0
38	318565.0
39	975641.0
40	1416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.312657148519943	13.348845693788233	46.03443818287226	12.304058974819569
2	25.784263077008056	13.010516563459804	49.135424949092815	12.069795410439326
3	25.98394642433593	13.891592644114795	44.89208419377733	15.232376737771947
4	23.691440491159767	13.924084123224398	47.36807415112529	15.016401234490539
5	23.010140590630108	16.53240723334591	44.71570187861091	15.741750297413073
6	25.498523726438453	18.55217040759632	43.97554598449413	11.973759881471084
7	86.0021602191968	2.6507476985589102	6.724204434529901	4.6228876477143865
8	87.33946308294935	2.6538111808749583	6.884433842938975	3.122291893236713
9	79.69407879926347	4.456484858274489	9.019866682819574	6.829569659642473
10	44.055428606705036	21.273192533829434	15.353802036844408	19.317576822621124
11	35.745686408025215	22.595595733590297	23.6008356808427	18.0578821775418
12	31.564265128612877	22.704859936196016	25.6409756541347	20.0898992810564
13	25.556033644462456	22.82633165166721	32.59856174146901	19.019072962401324
14	19.347979563787966	29.36788755720241	31.6398774421408	19.644255436868825
15	19.010810843427738	22.767522074478823	37.64318878802605	20.57847829406738
16	25.360202858229002	22.07916688134251	31.689171657589938	20.87145860283855
17	25.115217105742587	22.296627709383216	26.870453223642098	25.7177019612321
18	25.72833131654081	22.1983177768773	29.100621933326558	22.972728973255336
19	27.03806283944893	25.157966608971083	26.19244888742213	21.61152166415786
20	26.35272471222993	24.22230484341196	25.607973594639095	23.81699684971902
21	25.842747739405343	24.33351853476426	29.426557885194608	20.397175840635793
22	28.239783402516977	20.202598297167995	27.974885014976252	23.58273328533878
23	27.213795325033153	23.48307727726975	25.429316875934997	23.873810521762096
24	26.956880558073642	22.03335389579797	27.354529845976465	23.655235700151923
25	30.616303017390834	21.617880710783595	23.777542910800214	23.988273361025357
26	22.787481125931865	25.12083348998868	25.383178975599364	26.708506408480094
27	21.828843243002378	22.50044211619788	30.104005224629844	25.566709416169896
28	23.118058717672717	22.74092547800768	29.08233387222772	25.05868193209188
29	22.59109334291368	23.594476634216964	28.29130560510506	25.523124417764297
30	24.543506322609755	23.04820203758707	28.428280397751404	23.98001124205177
31	23.574610415561377	23.033720121183933	28.31210015173521	25.079569311519485
32	29.49311900097057	22.38565436214353	25.520571515834256	22.600655121051645
33	26.442261945376256	24.615869488226714	23.8255838834837	25.11628468291334
34	22.10061125755485	24.070755301565022	28.62963473543349	25.19899870544664
35	23.794902643924488	24.89766344490443	29.11292227898948	22.194511632181605
36	24.914141266452873	26.777713258983542	25.161401422476953	23.146744052086625
37	24.536451030003096	28.382281746611955	24.86493988380119	22.216327339583767
38	24.45652199139347	23.42570660844194	27.73268424641352	24.385087153751073
39	24.01886176778711	25.392880002933516	23.500297761197842	27.08796046808153
40	26.681306398825477	22.310088465014335	27.913429703060373	23.09517543309981
41	22.61202713874001	23.715855516890695	27.481942860484835	26.19017448388446
42	20.82880193240751	25.52358858175158	27.581088288167855	26.066521197673055
43	21.39610315766119	21.57294963681489	29.418481431815934	27.61246577370799
44	26.649279083703153	21.027417702564648	26.399187527356666	25.924115686375533
45	20.97677741155239	21.74018792143189	27.98245088796892	29.3005837790468
46	21.421585760562863	23.378222632543185	26.708413575682634	28.49177803121131
47	22.72291591530121	23.467527783695868	30.844671699132615	22.964884601870303
48	21.419868353809928	22.44307144737007	31.96896970912235	24.16809048969765
49	22.368433878215438	21.853072603138397	27.577467809067073	28.201025709579092
50	21.052343308681586	23.065840269103713	29.30573599930561	26.576080422909094
51	22.95936105042167	23.857611198606023	27.01452972529383	26.16849802567848
52	21.232160437353876	22.90236171278368	28.303095370381975	27.56238247948047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	223.5
2	164.0
3	160.0
4	156.0
5	225.5
6	295.0
7	374.5
8	454.0
9	593.5
10	733.0
11	848.0
12	963.0
13	1116.0
14	1402.0
15	1535.0
16	1396.5
17	1258.0
18	1314.0
19	1370.0
20	2173.5
21	2977.0
22	3765.0
23	4553.0
24	6119.0
25	7685.0
26	8907.0
27	10129.0
28	12336.0
29	14543.0
30	20369.5
31	26196.0
32	28563.5
33	30931.0
34	35858.5
35	40786.0
36	44160.0
37	47534.0
38	52184.0
39	70617.5
40	84401.0
41	96044.0
42	107687.0
43	126171.0
44	144655.0
45	173246.0
46	201837.0
47	210399.5
48	218962.0
49	204722.0
50	190482.0
51	168812.0
52	147142.0
53	140417.5
54	133693.0
55	134534.0
56	135375.0
57	129200.0
58	123025.0
59	116601.5
60	110178.0
61	99952.5
62	89727.0
63	79114.5
64	60009.5
65	51517.0
66	43395.0
67	35273.0
68	29194.0
69	23115.0
70	19021.0
71	14927.0
72	12701.0
73	10475.0
74	7717.5
75	4960.0
76	4506.0
77	4052.0
78	3035.0
79	2018.0
80	1390.0
81	762.0
82	632.5
83	503.0
84	349.5
85	196.0
86	174.0
87	152.0
88	99.0
89	37.0
90	28.0
91	29.0
92	30.0
93	32.5
94	35.0
95	19.0
96	3.0
97	2.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2154411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.945936654961596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.33989176015977	11.41137540119638
2	12.686496605568756	4.299691357027069
3	5.4350712034285795	2.7630711698551984
4	2.987285275301791	2.0248938818225466
5	1.811301749901113	1.5347102358422675
6	1.1788480857241401	1.1986010991902412
7	0.8211817374778468	0.9740985593857627
8	0.620678752598219	0.8414386259687999
9	0.4607834236012186	0.7027566037202327
>10	3.6788001683061067	13.435743142820641
>50	1.383169162113351	17.263741273374002
>100	1.5855365632377871	39.76565288485245
>500	0.005751644105203913	0.6862140694029847
>1k	0.003834429403469275	1.5122236597421743
>5k	0.0013694390726675981	1.585788035799241
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	8121	0.3769475740701287	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	7164	0.33252708048742785	No Hit
GTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	6941	0.3221762235710828	No Hit
TGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	6021	0.27947313674131813	No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	5920	0.27478508046978967	No Hit
TTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	4949	0.2297147573048968	No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	4483	0.20808471549764646	No Hit
ATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	4399	0.20418573800449402	No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	3278	0.1521529550304004	No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2701	0.12537069296434153	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39138307407453826	0.0
2	0.0	0.0	0.0	0.9142173893467866	0.0
3	0.0	0.0	0.0	3.1632311569148133	0.0
4	0.0	0.0	0.0	3.641877060598001	0.0
5	0.0	0.0	0.0	4.445484171775952	0.0
6	0.0	0.0	0.0	6.912747846163058	0.0
7	0.0	0.0	0.0	7.2527479668456944	0.0
8	0.0	0.0	0.0	7.858574803043616	0.0
9	0.0	0.0	0.0	8.245687568435178	0.0
10	0.0	0.0	0.0	9.592877125116795	0.0
11	0.0	0.0	0.0	10.736484356977382	0.0
12	0.0	0.0	0.0	11.198141858726121	0.0
13	0.0	0.0	0.0	11.434633410245306	0.0
14	0.0	0.0	0.0	11.59230991672434	0.0
15	0.0	0.0	0.0	11.82239600521906	0.0
16	0.0	0.0	0.0	12.316962733665953	0.0
17	0.0	0.0	0.0	12.956998455726414	0.0
18	4.641639872800501E-5	0.0	0.0	13.614904491297157	0.0
19	4.641639872800501E-5	0.0	0.0	14.098841864435338	0.0
20	4.641639872800501E-5	0.0	0.0	14.658623633095077	0.0
21	9.283279745601002E-5	0.0	0.0	15.459585009545533	0.0
22	9.283279745601002E-5	0.0	0.0	16.170730654457298	0.0
23	9.283279745601002E-5	0.0	0.0	16.849013489069634	0.0
24	9.283279745601002E-5	0.0	0.0	17.380852585695116	0.0
25	9.283279745601002E-5	0.0	0.0	17.854996098701687	0.0
26	9.283279745601002E-5	0.0	0.0	18.2788242354871	0.0
27	9.283279745601002E-5	0.0	0.0	18.75143600733565	0.0
28	9.283279745601002E-5	0.0	0.0	19.15618700424385	0.0
29	9.283279745601002E-5	0.0	0.0	19.655441788962275	0.0
30	9.283279745601002E-5	0.0	0.0	20.159059715161128	0.0
31	9.283279745601002E-5	0.0	0.0	20.636081044888837	0.0
32	9.283279745601002E-5	0.0	0.0	21.101869606124364	0.0
33	9.747443732881052E-4	0.0	0.0	21.579680014630448	0.0
34	9.747443732881052E-4	0.0	0.0	22.0743395758748	0.0
35	9.747443732881052E-4	0.0	0.0	22.616158198226803	0.0
36	9.747443732881052E-4	0.0	0.0	23.089930380043548	0.0
37	9.747443732881052E-4	0.0	0.0	23.59452305061569	0.0
38	9.747443732881052E-4	0.0	0.0	24.166094584552344	0.0
39	9.747443732881052E-4	0.0	0.0	25.315875197443756	0.0
40	9.747443732881052E-4	0.0	0.0	25.82111769759809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAC	35	1.02012564E-7	46.000004	46
ATCGTAC	35	1.02012564E-7	46.000004	12
CGGAATT	85	0.0	46.0	14
CTAGACG	30	1.8621668E-6	46.0	25
AGCGTAT	20	6.31269E-4	46.0	34
CGTGATA	20	6.31269E-4	46.0	20
CGACACG	20	6.31269E-4	46.0	18
GTTCGAC	25	3.4181576E-5	46.0	18
ACAATCG	20	6.31269E-4	46.0	33
ACGTATA	25	3.4181576E-5	46.0	17
CGATGCG	25	3.4181576E-5	46.0	1
ACCCGTT	20	6.31269E-4	46.0	26
CAATCGT	20	6.31269E-4	46.0	9
ATCATCG	25	3.4181576E-5	46.0	14
CCGTTGA	20	6.31269E-4	46.0	28
TACCGGA	30	1.8621668E-6	46.0	11
TTTAGCG	135	0.0	44.2963	1
ATTAGCG	55	4.7293724E-11	41.81818	1
TTTACGC	45	1.589433E-8	40.88889	25
TATTCCG	45	1.589433E-8	40.88889	13
>>END_MODULE