FastQCFastQC Report
Thu 26 May 2016
SRR1545630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545630_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1839667
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC88500.481065323235129No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43790.23803220909001466No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG42810.2327051580530607No Hit
ATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT36880.20047106351312494No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36250.19704653070365452No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT31640.17198764776451392No Hit
ATTGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC29730.1616053340088179No Hit
ATGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC28390.15432140708073797No Hit
ATATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC27570.1498640786620622No Hit
ATGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC24730.13442650218762417No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC23120.1256749183411998No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC23120.1256749183411998No Hit
ATGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC20960.11393366299444412No Hit
ATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC20790.11300958271252351No Hit
ATTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC20430.11105270682139758No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC19280.104801575502523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC850.045.00000422
ATCGAAT1250.045.0000041
TCACGGT406.8193913E-945.041
CTATGCG502.1827873E-1145.03
AACCGGC453.8562575E-1045.045
AAATCGG207.0343935E-445.07
GTCGACA351.2124656E-745.035
CGAAATC253.891722E-545.042
CGAAATA253.891722E-545.036
GGTCGCT453.8562575E-1045.033
GGTCGAC207.0343935E-445.011
CGAGTAC406.8193913E-945.027
CACGGCA351.2124656E-745.023
CGACGAA406.8193913E-945.05
GCGCGTT253.891722E-545.024
AACCCAC207.0343935E-445.028
TAGCGAG502.1827873E-1145.028
CACGCTA502.1827873E-1145.036
ATTGTCG1450.045.01
GTCTAAT207.0343935E-445.031