##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545630_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1839667
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013037685624624	31.0	31.0	34.0	31.0	34.0
2	32.67428507441836	34.0	31.0	34.0	31.0	34.0
3	32.61431933061799	34.0	31.0	34.0	31.0	34.0
4	36.04576371702053	37.0	35.0	37.0	35.0	37.0
5	35.816727701263325	37.0	35.0	37.0	35.0	37.0
6	35.80858546682634	37.0	35.0	37.0	35.0	37.0
7	35.78089512939027	37.0	35.0	37.0	35.0	37.0
8	35.75748165292958	37.0	35.0	37.0	33.0	37.0
9	37.98036927335219	39.0	38.0	39.0	35.0	39.0
10	37.95574362099228	39.0	39.0	39.0	35.0	39.0
11	37.7755724269664	39.0	38.0	39.0	35.0	39.0
12	37.205035476529176	39.0	37.0	39.0	33.0	39.0
13	36.956245885804336	39.0	37.0	39.0	33.0	39.0
14	37.686947692163855	40.0	36.0	41.0	33.0	41.0
15	37.58006258741392	40.0	36.0	41.0	33.0	41.0
16	37.624243952845816	40.0	36.0	41.0	33.0	41.0
17	37.71446680295945	40.0	35.0	41.0	33.0	41.0
18	37.79727037556253	40.0	35.0	41.0	33.0	41.0
19	37.788701433465945	40.0	35.0	41.0	33.0	41.0
20	37.755914521486766	40.0	36.0	41.0	33.0	41.0
21	37.72585690779908	40.0	36.0	41.0	33.0	41.0
22	37.64502651838621	40.0	35.0	41.0	33.0	41.0
23	37.58686273113558	40.0	35.0	41.0	33.0	41.0
24	37.56776199170828	40.0	35.0	41.0	33.0	41.0
25	37.54137569462299	40.0	35.0	41.0	33.0	41.0
26	37.44513110253106	39.0	35.0	41.0	33.0	41.0
27	37.339697347400374	39.0	35.0	41.0	32.0	41.0
28	37.273856083736895	39.0	35.0	41.0	32.0	41.0
29	37.21571893174145	39.0	35.0	41.0	32.0	41.0
30	37.19283326819473	39.0	35.0	41.0	32.0	41.0
31	37.16931325071331	39.0	35.0	41.0	32.0	41.0
32	37.05381843561906	39.0	35.0	41.0	31.0	41.0
33	36.936997837108564	39.0	35.0	41.0	31.0	41.0
34	36.892840932625305	39.0	35.0	41.0	31.0	41.0
35	36.78324446761289	39.0	35.0	41.0	31.0	41.0
36	36.73071757008198	39.0	35.0	41.0	31.0	41.0
37	36.62607852399375	39.0	35.0	41.0	31.0	41.0
38	36.50392217722012	39.0	35.0	41.0	30.0	41.0
39	36.456573390727776	39.0	35.0	41.0	30.0	41.0
40	36.432094504059705	38.0	35.0	41.0	30.0	41.0
41	36.3811885520586	38.0	35.0	41.0	30.0	41.0
42	36.29215015543574	38.0	35.0	41.0	30.0	41.0
43	36.22392259033836	38.0	35.0	40.0	30.0	41.0
44	36.148278465613615	38.0	35.0	40.0	30.0	41.0
45	36.13676877391397	38.0	35.0	40.0	30.0	41.0
46	36.054674568821426	38.0	35.0	40.0	30.0	41.0
47	35.948394464867825	38.0	35.0	40.0	30.0	41.0
48	35.909812482367734	38.0	35.0	40.0	30.0	41.0
49	35.90595308824912	38.0	35.0	40.0	30.0	41.0
50	35.82335226973142	38.0	35.0	40.0	29.0	41.0
51	35.67079639956579	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	12.0
12	10.0
13	26.0
14	15.0
15	37.0
16	57.0
17	170.0
18	307.0
19	643.0
20	1237.0
21	1952.0
22	3187.0
23	4932.0
24	7570.0
25	11240.0
26	15694.0
27	19142.0
28	22815.0
29	27703.0
30	34035.0
31	42590.0
32	53514.0
33	69266.0
34	111791.0
35	228191.0
36	124969.0
37	173513.0
38	267754.0
39	616888.0
40	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.547617041562413	92.33589557240522	3.2775496869813936	2.8389376990509696
2	3.0756109665499247	2.550407220437177	92.22152704810163	2.1524547649112584
3	25.65882847276165	15.299073147477232	45.103380122598274	13.938718257162847
4	25.069319610559955	15.405233664570817	46.43590388912776	13.089542835741469
5	23.485554722675353	14.234315232050148	48.52514069122292	13.754989354051576
6	21.065225391334412	13.627411917482894	51.015591408662544	14.291771282520152
7	21.458774876105295	16.71166575255196	47.85757422403076	13.971985147311985
8	23.725598165320136	19.259083301488804	45.21736814325636	11.7979503899347
9	84.30656200279725	3.6223403474650575	8.22426015142958	3.8468374983081177
10	85.44633349405083	3.4383940136992184	7.7948346086547184	3.3204378835952375
11	79.10295721997514	5.832903454810028	9.17209473236189	5.892044592852946
12	39.84210185865159	27.65500495470104	16.246146721118553	16.256746465528813
13	30.85183351117349	22.94377188915168	27.10718842051306	19.09720617916177
14	28.115251292761133	23.660477684276557	28.83364217545893	19.39062884750338
15	19.75330317932539	32.135163592106615	31.00756821750893	17.103965011059067
16	15.746273646263157	36.52383828160205	28.545383485163345	19.184504586971446
17	14.139895970303321	25.32898616977964	40.31169771485818	20.219420145058862
18	17.685320223714402	21.45540470095947	37.83086830388326	23.028406771442874
19	17.598402319550225	22.380028559516475	29.031123567471724	30.990445553461576
20	19.748302274270287	22.423242902112175	35.016500268798644	22.81195455481889
21	23.349443132914814	27.018259282794116	28.14672438001008	21.485573204280993
22	25.238045798505926	22.643826301172982	28.77085907395197	23.34726882636912
23	21.59097271408358	26.35607422430255	28.77792557022548	23.275027491388386
24	25.720252632677543	21.351581563402508	25.21766167464003	27.71050412927992
25	22.001536147574534	26.97085940009795	24.13665081778387	26.890953634543642
26	21.59396238558391	22.453520120760988	35.10129822408077	20.85121926957433
27	23.301499673582228	26.941125758085565	28.969645049892183	20.78772951844002
28	21.917336126592478	29.459842460619235	25.22282565268606	23.399995760102236
29	18.144370693174363	30.195084219046166	26.344441684283083	25.316103403496392
30	18.204707699817412	27.431921103112682	30.943263101419987	23.42010809564992
31	19.633988107630348	23.763050595569744	30.50503161713506	26.09792967966485
32	20.060695767223088	30.068757008741255	29.315903367294187	20.554643856741464
33	23.263558024359842	30.68310732322752	25.608221487910583	20.445113164502054
34	25.851308959719336	25.93659613397425	24.838245182416166	23.373849723890245
35	24.21791552492924	28.422535165331553	24.62907689272026	22.730472417018948
36	20.11325962796528	26.418748610482222	26.855240649530593	26.612751112021904
37	21.10854844925739	27.165785981919555	29.617044823873016	22.108620744950038
38	24.040600826127772	28.91648325484993	24.636578250302907	22.40633766871939
39	22.106990015040765	29.691840969044943	28.598436564878316	19.602732451035976
40	20.850023400974198	27.259118090393535	30.34043661162591	21.550421897006363
41	19.363178227363974	30.310376823631668	26.61758894408608	23.70885600491828
42	23.395918935329057	24.84221329186206	29.195555499989943	22.56631227281894
43	19.518043211081135	25.692312793565357	28.345673428941215	26.44397056641229
44	18.677238869860687	27.89885343380079	26.54839163826932	26.875516058069206
45	20.22599742235959	24.4852465147225	28.521194324842487	26.767561738075425
46	22.237937626755276	25.326377001924804	29.1837055293159	23.251979842004015
47	18.777420043953608	30.3069522908222	26.88698552509775	24.028642140126447
48	19.195865338672704	28.78254597163508	27.778940427805683	24.242648261886526
49	21.15246944148044	27.33494703117466	28.625724112026795	22.8868594153181
50	20.416847179408013	24.95103733447412	30.812315489705473	23.819799996412392
51	19.855658659964003	23.69200512918914	30.454913851256777	25.99742235959008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1066.0
1	1307.0
2	1548.0
3	4249.5
4	6951.0
5	4849.5
6	2748.0
7	2825.5
8	2903.0
9	3062.0
10	3221.0
11	3467.5
12	3714.0
13	3609.0
14	3504.0
15	3859.0
16	4214.0
17	4039.0
18	3864.0
19	5028.0
20	6192.0
21	6627.0
22	7062.0
23	7311.0
24	7560.0
25	11494.5
26	18379.0
27	21329.0
28	24765.5
29	28202.0
30	33827.0
31	39452.0
32	50989.5
33	62527.0
34	73955.0
35	85383.0
36	86476.0
37	87569.0
38	101901.5
39	116234.0
40	128366.5
41	140499.0
42	148627.0
43	156755.0
44	169919.0
45	183083.0
46	190380.0
47	197677.0
48	182963.0
49	168249.0
50	140293.5
51	112338.0
52	95973.5
53	79609.0
54	73856.0
55	68103.0
56	64308.5
57	60514.0
58	55563.0
59	50612.0
60	45302.5
61	39993.0
62	33743.0
63	27493.0
64	22920.0
65	18347.0
66	14143.5
67	9940.0
68	9021.0
69	8102.0
70	5301.0
71	2500.0
72	2492.5
73	2485.0
74	1870.0
75	1170.5
76	1086.0
77	643.0
78	200.0
79	135.5
80	71.0
81	49.5
82	28.0
83	22.5
84	17.0
85	19.5
86	22.0
87	19.0
88	16.0
89	8.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1839667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.784634611451963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.95328036804622	10.194490555025675
2	12.09753056940475	3.577151383390393
3	5.484853805260663	2.432746782240324
4	3.1234775543147815	1.8471789743046259
5	1.9573042173144932	1.4469013838224376
6	1.2593954885959737	1.1171821278121494
7	0.9115145915646078	0.9433487125502722
8	0.6516258471248366	0.7707240042494853
9	0.5015957399232589	0.6674318763683645
>10	2.796286364265171	8.127926604197237
>50	0.5791561223275182	6.236417216007325
>100	1.6356548428164117	55.586835575078766
>500	0.038733369078872794	3.525614246777791
>1k	0.00922223073306495	3.0433810747895285
>5k	3.6888922932259803E-4	0.48266948338567306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	8850	0.481065323235129	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4379	0.23803220909001466	No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	4281	0.2327051580530607	No Hit
ATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	3688	0.20047106351312494	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3625	0.19704653070365452	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	3164	0.17198764776451392	No Hit
ATTGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2973	0.1616053340088179	No Hit
ATGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2839	0.15432140708073797	No Hit
ATATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2757	0.1498640786620622	No Hit
ATGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2473	0.13442650218762417	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2312	0.1256749183411998	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2312	0.1256749183411998	No Hit
ATGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2096	0.11393366299444412	No Hit
ATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2079	0.11300958271252351	No Hit
ATTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2043	0.11105270682139758	No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	1928	0.104801575502523	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.184489910402263	0.0
2	0.0	0.0	0.0	0.8474903338484628	0.0
3	0.0	0.0	0.0	1.1704835712115291	0.0
4	0.0	0.0	0.0	1.5782747638567198	0.0
5	0.0	0.0	0.0	3.0821882438506534	0.0
6	0.0	0.0	0.0	3.413933065060144	0.0
7	0.0	0.0	0.0	3.9456053731463356	0.0
8	0.0	0.0	0.0	5.589598552346702	0.0
9	0.0	0.0	0.0	5.7964838201696285	0.0
10	5.435766364238745E-5	0.0	0.0	6.213461457970383	0.0
11	5.435766364238745E-5	0.0	0.0	6.543358118616032	0.0
12	5.435766364238745E-5	0.0	0.0	7.421832320740656	0.0
13	1.087153272847749E-4	0.0	0.0	8.205724188127526	0.0
14	1.6307299092716237E-4	0.0	0.0	8.551167140574897	0.0
15	1.6307299092716237E-4	0.0	0.0	8.733971963404246	0.0
16	1.6307299092716237E-4	0.0	0.0	8.83513157544273	0.0
17	2.174306545695498E-4	0.0	0.0	8.987278675977771	0.0
18	2.174306545695498E-4	0.0	0.0	9.354464693882099	0.0
19	2.174306545695498E-4	0.0	0.0	9.937070132801209	0.0
20	2.717883182119373E-4	0.0	0.0	10.598602899329064	0.0
21	2.717883182119373E-4	0.0	0.0	10.992261099427234	0.0
22	2.717883182119373E-4	0.0	0.0	11.383962423634276	0.0
23	3.2614598185432475E-4	0.0	0.0	12.015815905813389	0.0
24	3.2614598185432475E-4	0.0	0.0	12.544770330717462	0.0
25	3.2614598185432475E-4	0.0	0.0	13.143628711065643	0.0
26	3.8050364549671215E-4	0.0	0.0	13.569575363367392	0.0
27	3.8050364549671215E-4	0.0	0.0	13.95111180447331	0.0
28	3.8050364549671215E-4	0.0	0.0	14.280247457827965	0.0
29	3.8050364549671215E-4	0.0	0.0	14.60003359303613	0.0
30	3.8050364549671215E-4	0.0	0.0	14.96678474963132	0.0
31	3.8050364549671215E-4	0.0	0.0	15.357888139538296	0.0
32	4.348613091390996E-4	0.0	0.0	15.81721039731647	0.0
33	5.435766364238746E-4	0.0	0.0	16.18330926194795	0.0
34	5.97934300066262E-4	0.0	0.0	16.537829944223603	0.0
35	5.97934300066262E-4	0.0	0.0	16.927411319548593	0.0
36	5.97934300066262E-4	0.0	0.0	17.285900111270138	0.0
37	5.97934300066262E-4	0.0	0.0	17.787186485380236	0.0
38	5.97934300066262E-4	0.0	0.0	18.169755722095356	0.0
39	5.97934300066262E-4	0.0	0.0	18.54281236767306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCC	85	0.0	45.000004	22
ATCGAAT	125	0.0	45.000004	1
TCACGGT	40	6.8193913E-9	45.0	41
CTATGCG	50	2.1827873E-11	45.0	3
AACCGGC	45	3.8562575E-10	45.0	45
AAATCGG	20	7.0343935E-4	45.0	7
GTCGACA	35	1.2124656E-7	45.0	35
CGAAATC	25	3.891722E-5	45.0	42
CGAAATA	25	3.891722E-5	45.0	36
GGTCGCT	45	3.8562575E-10	45.0	33
GGTCGAC	20	7.0343935E-4	45.0	11
CGAGTAC	40	6.8193913E-9	45.0	27
CACGGCA	35	1.2124656E-7	45.0	23
CGACGAA	40	6.8193913E-9	45.0	5
GCGCGTT	25	3.891722E-5	45.0	24
AACCCAC	20	7.0343935E-4	45.0	28
TAGCGAG	50	2.1827873E-11	45.0	28
CACGCTA	50	2.1827873E-11	45.0	36
ATTGTCG	145	0.0	45.0	1
GTCTAAT	20	7.0343935E-4	45.0	31
>>END_MODULE