FastQCFastQC Report
Thu 26 May 2016
SRR1545629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545629_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1514997
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT73540.4854135024689818Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT55520.3664693725466123No Hit
TGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC51730.3414528213587222No Hit
TTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC51090.3372283905512684No Hit
GTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC49060.3238290240838761No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC44020.29056163147517783No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC41040.27089162552797136No Hit
GGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC35750.2359740646351115No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC28580.18864723824535626No Hit
AGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC27270.18000035643634937No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT19060.1258088299844818No Hit
TCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC18950.1250827559394507No Hit
CTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC18020.1189441299223695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.0198528E-746.0000041
ACGCACC351.0198528E-746.00000420
TAGCGTG351.0198528E-746.00000434
CTAAGCG351.0198528E-746.00000422
TAACGCG351.0198528E-746.00000416
AGACGCA351.0198528E-746.00000440
CGCTAGA351.0198528E-746.00000415
TCGTCCG351.0198528E-746.00000420
TTAACGA351.0198528E-746.00000411
TCCCGAA351.0198528E-746.00000437
CCGCGAT351.0198528E-746.00000443
CGCGTAG700.046.0000041
CATTTCG351.0198528E-746.00000413
AGTGTCG351.0198528E-746.00000430
ACGCGAA206.3120935E-446.038
AACGTAC301.8617975E-646.028
TTAGGCG301.8617975E-646.01
AGGTACG253.4176745E-546.01
TCGTTAT301.8617975E-646.015
AGTCCGC206.3120935E-446.037
Produced by FastQC (version 0.11.5)