SRR1545628_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545628_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2991740
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	11818	0.39502095770354373	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7181	0.24002754250035097	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	6928	0.2315709252809402	RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	6459	0.21589442932875183	RNA PCR Primer, Index 41 (96% over 27bp)
ATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	5582	0.1865803846590947	RNA PCR Primer, Index 41 (95% over 24bp)
ATTGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	4578	0.1530213186974804	Illumina PCR Primer Index 11 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	4293	0.14349508981395442	Illumina PCR Primer Index 11 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4054	0.13550642769759405	RNA PCR Primer, Index 41 (96% over 26bp)
ATGTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3838	0.12828654896481648	RNA PCR Primer, Index 41 (95% over 23bp)
ATATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3673	0.12277136382172248	RNA PCR Primer, Index 41 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3473	0.11608629092100249	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3378	0.1129108812931605	RNA PCR Primer, Index 41 (95% over 23bp)
ATAGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3100	0.10361862996115971	RNA PCR Primer, Index 41 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	135	0.0	45.000004	1
CAACGTT	20	7.035102E-4	45.0	8
GTTTCGA	25	3.8923114E-5	45.0	11
ACGCATA	40	6.8212103E-9	45.0	15
GCGAATC	40	6.8212103E-9	45.0	21
CGCATAC	25	3.8923114E-5	45.0	26
TACGGAC	35	1.2128112E-7	45.0	32
GTTACGT	35	1.2128112E-7	45.0	45
CGTACGA	35	1.2128112E-7	45.0	24
CGTGTAA	20	7.035102E-4	45.0	35
CGTCCTA	45	3.8562575E-10	45.0	23
GATTCGA	20	7.035102E-4	45.0	23
CAATCGA	35	1.2128112E-7	45.0	32
AATACGC	20	7.035102E-4	45.0	15
GTAACGT	25	3.8923114E-5	45.0	24
AATCGAC	35	1.2128112E-7	45.0	33
TACCGGA	20	7.035102E-4	45.0	34
CTTCGAA	30	2.1666747E-6	44.999996	30
AACCGTA	30	2.1666747E-6	44.999996	22
CCTATCG	30	2.1666747E-6	44.999996	37