##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545628_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2991740
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91732470067586	31.0	31.0	34.0	31.0	34.0
2	32.58927881433547	34.0	31.0	34.0	31.0	34.0
3	32.543327962991434	34.0	31.0	34.0	31.0	34.0
4	36.01698175643605	37.0	35.0	37.0	35.0	37.0
5	35.78137037309392	37.0	35.0	37.0	35.0	37.0
6	35.77834671462092	37.0	35.0	37.0	35.0	37.0
7	35.76112930936512	37.0	35.0	37.0	33.0	37.0
8	35.72530935174848	37.0	35.0	37.0	33.0	37.0
9	37.922858269769435	39.0	38.0	39.0	35.0	39.0
10	37.851360078081655	39.0	38.0	39.0	35.0	39.0
11	37.68229291315422	39.0	38.0	39.0	35.0	39.0
12	37.192281080575185	39.0	37.0	39.0	33.0	39.0
13	36.99485550214925	39.0	37.0	39.0	33.0	39.0
14	37.78756776992653	40.0	36.0	41.0	33.0	41.0
15	37.75183939780863	40.0	36.0	41.0	33.0	41.0
16	37.73197704345966	40.0	36.0	41.0	33.0	41.0
17	37.79614973226283	40.0	36.0	41.0	33.0	41.0
18	37.85141489567944	40.0	36.0	41.0	33.0	41.0
19	37.81726353225882	40.0	36.0	41.0	33.0	41.0
20	37.780003609939364	40.0	36.0	41.0	33.0	41.0
21	37.761672805791946	40.0	36.0	41.0	33.0	41.0
22	37.688226918114545	40.0	35.0	41.0	33.0	41.0
23	37.62077419829263	40.0	35.0	41.0	32.0	41.0
24	37.58416540207371	40.0	35.0	41.0	32.0	41.0
25	37.49970084298769	40.0	35.0	41.0	32.0	41.0
26	37.420993802937424	40.0	35.0	41.0	32.0	41.0
27	37.33705736461056	40.0	35.0	41.0	32.0	41.0
28	37.23728398858189	39.0	35.0	41.0	32.0	41.0
29	37.17600660485203	39.0	35.0	41.0	31.0	41.0
30	37.11619291783377	39.0	35.0	41.0	31.0	41.0
31	37.03193659876861	39.0	35.0	41.0	31.0	41.0
32	36.89572823841644	39.0	35.0	41.0	31.0	41.0
33	36.82603802469466	39.0	35.0	41.0	31.0	41.0
34	36.742369657791116	39.0	35.0	41.0	31.0	41.0
35	36.61848656634601	39.0	35.0	41.0	30.0	41.0
36	36.55855956734208	39.0	35.0	41.0	30.0	41.0
37	36.42352978534231	39.0	35.0	41.0	30.0	41.0
38	36.345090816715356	38.0	35.0	41.0	30.0	41.0
39	36.308521128172906	38.0	35.0	41.0	30.0	41.0
40	36.25778944694392	38.0	35.0	41.0	30.0	41.0
41	36.2015823567556	38.0	35.0	41.0	30.0	41.0
42	36.063287250897474	38.0	35.0	40.0	30.0	41.0
43	35.951550268405676	38.0	35.0	40.0	29.0	41.0
44	35.83796553176413	38.0	35.0	40.0	29.0	41.0
45	35.84997025142559	38.0	35.0	40.0	29.0	41.0
46	35.74577637094133	38.0	35.0	40.0	29.0	41.0
47	35.6520573311852	37.0	34.0	40.0	29.0	41.0
48	35.5913912973721	37.0	34.0	40.0	29.0	41.0
49	35.543148803037695	37.0	34.0	40.0	29.0	41.0
50	35.4295667404253	37.0	34.0	40.0	28.0	41.0
51	35.243617426648036	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	21.0
11	25.0
12	25.0
13	36.0
14	46.0
15	72.0
16	138.0
17	332.0
18	730.0
19	1337.0
20	2466.0
21	4079.0
22	6445.0
23	9499.0
24	13776.0
25	19990.0
26	26827.0
27	33428.0
28	39959.0
29	48145.0
30	57948.0
31	72840.0
32	90744.0
33	117292.0
34	183600.0
35	314742.0
36	222651.0
37	312214.0
38	470162.0
39	941641.0
40	515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.1292291442438183	91.35105323323552	3.303027669516736	3.2166899530039372
2	3.02934078496126	2.521208393777534	91.4855234746335	2.963927346627715
3	26.921423653124936	16.12132070300227	44.14427724334334	12.812978400529456
4	27.215633711485626	15.599818166017101	43.74016458649482	13.44438353600246
5	24.196287110510937	13.505050572576494	47.65561178444651	14.643050532466056
6	22.40592431160462	14.723806213106755	48.081751756502904	14.788517718785723
7	22.738105584041392	17.328578018143286	44.70381784513361	15.229498552681717
8	25.515953926477568	19.016024119743026	43.981829971855845	11.486191981923563
9	82.72283019246325	3.8876707200492024	9.26397347363073	4.125525613856819
10	83.46878405209009	4.196922192436508	8.604491031974703	3.7298027234987
11	76.75045291368903	6.542781124028157	10.082527224959389	6.624238737323431
12	41.361649073783155	24.096913501841737	16.054904503733614	18.486532920641498
13	31.859018497596715	23.30249286368468	24.244887590499175	20.59360104821943
14	25.408625081056506	23.278760854887125	30.23999411713585	21.072619946920522
15	21.12376075461103	25.66710342476285	33.28006444410276	19.92907137652336
16	17.171044275237822	31.110424034174095	31.319232286228083	20.399299404360004
17	17.1243824663908	22.67556672705516	39.579976869647766	20.620073936906284
18	21.742363975479154	20.105156196728323	34.805698356140574	23.346781471651948
19	20.437103491613577	21.27781825960812	30.162647823674515	28.122430425103783
20	22.607011304458275	21.737550722990633	31.51019139363715	24.14524657891394
21	25.162781525132534	23.639052858871427	28.355405215693878	22.842760400302165
22	26.052197049208818	21.638812196247002	26.604751749817833	25.704239004726347
23	24.341553744643583	23.666495083128883	28.484661100229296	23.507290071998234
24	27.944306657664104	21.032108405142157	25.895632641873963	25.127952295319783
25	26.31689251071283	23.182061275378206	25.007687833835828	25.493358380073133
26	26.525132531570257	20.797529197055894	29.50690902284289	23.170429248530954
27	27.745826843241726	23.138274047878493	25.384759370800943	23.731139738078845
28	23.84047410537012	25.53918455480757	25.071730832224727	25.548610507597587
29	21.376389659529238	24.07298094085716	27.595613255162547	26.955016144451054
30	21.725383890311324	23.803104547855096	28.608301523528112	25.863210038305468
31	22.693917252167633	23.227319218916083	28.38261346239981	25.696150066516477
32	22.13471090402241	25.796359309298268	29.172387974890867	22.896541811788456
33	24.217612493064237	25.66265785128387	24.977504729689077	25.142224925962818
34	26.91353526710209	24.86332368454478	24.00382386169921	24.21931718665392
35	24.07475248517585	26.59301944687707	24.71608495390642	24.61614311404066
36	22.482869500691905	24.124589703650717	25.942261025356483	27.450279770300895
37	22.12478357076484	25.444624198626887	28.36125465448201	24.06933757612627
38	24.582751174901563	26.099727917532938	26.22981943618095	23.087701471384545
39	24.45058728365433	27.010034294423978	25.39127731687914	23.14810110504255
40	22.851217017521577	25.241097154164464	28.06263244800685	23.845053380307114
41	21.691390294611164	27.747698663653924	24.092267376175737	26.46864366555917
42	23.671007507336867	23.5199248597806	28.88178785589657	23.92727977698597
43	22.006056676048054	24.35542527091258	27.274997158844016	26.363520894195354
44	21.433279629914363	25.26075126849258	26.160027275097438	27.14594182649562
45	22.103391337482535	22.69378355070962	28.005207671789663	27.197617440018185
46	23.445352871573064	23.21130846931886	27.81942949587865	25.523909163229426
47	20.30005949714882	26.28931658499736	26.74453662417189	26.66608729368194
48	21.569588266360046	24.58358680901415	27.484373642094567	26.362451282531236
49	22.621283935101314	24.47204636766564	29.297432263498834	23.609237433734215
50	20.79869908481352	23.65078516181219	29.430130960578126	26.120384792796163
51	20.62822972584516	24.282691677752748	28.6768903714895	26.41218822491259
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1297.0
1	2049.0
2	2801.0
3	7357.5
4	11914.0
5	8163.5
6	4413.0
7	4559.5
8	4706.0
9	4926.0
10	5146.0
11	5040.5
12	4935.0
13	4983.0
14	5031.0
15	5300.5
16	5570.0
17	6201.5
18	6833.0
19	8073.0
20	9313.0
21	9542.5
22	9772.0
23	11679.5
24	13587.0
25	17129.5
26	23798.0
27	26924.0
28	30496.0
29	34068.0
30	38889.5
31	43711.0
32	57528.0
33	71345.0
34	85761.5
35	100178.0
36	102018.0
37	103858.0
38	121145.0
39	138432.0
40	150433.5
41	162435.0
42	180800.5
43	199166.0
44	224486.5
45	249807.0
46	271620.5
47	293434.0
48	281471.0
49	269508.0
50	232039.5
51	194571.0
52	187293.5
53	180016.0
54	175843.5
55	171671.0
56	168758.0
57	165845.0
58	154159.5
59	142474.0
60	127828.0
61	113182.0
62	94495.5
63	75809.0
64	66681.0
65	57553.0
66	46258.5
67	34964.0
68	29107.0
69	23250.0
70	18760.0
71	14270.0
72	12142.5
73	10015.0
74	7219.5
75	3135.5
76	1847.0
77	1916.0
78	1985.0
79	1336.0
80	687.0
81	443.0
82	199.0
83	125.5
84	52.0
85	35.5
86	19.0
87	16.0
88	13.0
89	11.5
90	10.0
91	10.0
92	10.0
93	10.5
94	11.0
95	9.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2991740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.121887990941145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.8072904099359	9.858107361202148
2	11.477422023194823	3.241657364726368
3	4.995935493123825	2.1165612433158594
4	2.942586857981892	1.662195280481429
5	1.8933513629500225	1.3368847937537989
6	1.26308238872166	1.07022648101146
7	0.916201376491069	0.9056945251167083
8	0.7075570075365158	0.7993632646108941
9	0.5075829936383582	0.6451227164040739
>10	2.985944258016362	8.315912105910638
>50	0.6409251099945011	6.599727207088922
>100	1.8273769968732752	57.77001500460587
>500	0.027128659286101375	2.3672706869465787
>1k	0.0064252087782871664	2.0353110627783506
>5k	9.518827819684691E-4	0.8787957250454704
>10k+	2.3797069549211727E-4	0.39715517700142905
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	11818	0.39502095770354373	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7181	0.24002754250035097	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	6928	0.2315709252809402	RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	6459	0.21589442932875183	RNA PCR Primer, Index 41 (96% over 27bp)
ATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	5582	0.1865803846590947	RNA PCR Primer, Index 41 (95% over 24bp)
ATTGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	4578	0.1530213186974804	Illumina PCR Primer Index 11 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	4293	0.14349508981395442	Illumina PCR Primer Index 11 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4054	0.13550642769759405	RNA PCR Primer, Index 41 (96% over 26bp)
ATGTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3838	0.12828654896481648	RNA PCR Primer, Index 41 (95% over 23bp)
ATATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3673	0.12277136382172248	RNA PCR Primer, Index 41 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3473	0.11608629092100249	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3378	0.1129108812931605	RNA PCR Primer, Index 41 (95% over 23bp)
ATAGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3100	0.10361862996115971	RNA PCR Primer, Index 41 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23106954481338618	0.0
2	0.0	0.0	0.0	0.8227653472561118	0.0
3	0.0	0.0	0.0	1.1495985613723119	0.0
4	0.0	0.0	0.0	1.5246311511027029	0.0
5	0.0	0.0	0.0	2.8673948939413183	0.0
6	0.0	0.0	0.0	3.184902431361014	0.0
7	0.0	0.0	0.0	3.679798378201314	0.0
8	3.342536450359991E-5	0.0	0.0	5.214791392300133	0.0
9	3.342536450359991E-5	0.0	0.0	5.409828394178638	0.0
10	3.342536450359991E-5	0.0	0.0	5.779880604597993	0.0
11	6.685072900719982E-5	0.0	0.0	6.053266660872937	0.0
12	1.0027609351079973E-4	0.0	0.0	6.846851664917406	0.0
13	1.3370145801439964E-4	0.0	0.0	7.539324941338485	0.0
14	1.3370145801439964E-4	0.0	0.0	7.854158449597893	0.0
15	2.0055218702159946E-4	0.0	0.0	8.014633624579677	0.0
16	2.0055218702159946E-4	0.0	0.0	8.124770200619038	0.0
17	2.0055218702159946E-4	0.0	0.0	8.28263819716954	0.0
18	2.0055218702159946E-4	0.0	0.0	8.618629961159726	0.0
19	2.674029160287993E-4	0.0	0.0	9.07261326184762	0.0
20	2.674029160287993E-4	0.0	0.0	9.57205505826041	0.0
21	3.3425364503599913E-4	0.0	0.0	9.896214243216322	0.0
22	3.3425364503599913E-4	0.0	0.0	10.30631004031099	0.0
23	3.67679009539599E-4	0.0	0.0	10.84131642455561	0.0
24	4.3452973854679887E-4	0.0	0.0	11.29693756810418	0.0
25	4.3452973854679887E-4	0.0	0.0	11.80520366074592	0.0
26	5.013804675539987E-4	0.0	0.0	12.183344809375146	0.0
27	5.013804675539987E-4	0.0	0.0	12.504595987619245	0.0
28	5.348058320575986E-4	0.0	0.0	12.801078970766175	0.0
29	5.348058320575986E-4	0.0	0.0	13.103143989785208	0.0
30	5.348058320575986E-4	0.0	0.0	13.438333545027309	0.0
31	5.348058320575986E-4	0.0	0.0	13.793277490691036	0.0
32	5.682311965611985E-4	0.0	0.0	14.17566366061222	0.0
33	5.682311965611985E-4	0.0	0.0	14.519410109167241	0.0
34	6.016565610647985E-4	0.0	0.0	14.856906014560089	0.0
35	6.016565610647985E-4	0.0	0.0	15.228830045391645	0.0
36	6.350819255683983E-4	0.0	0.0	15.583138909129804	0.0
37	6.350819255683983E-4	0.0	0.0	15.999284697199624	0.0
38	7.35358019079198E-4	0.0	0.0	16.385548209403222	0.0
39	7.68783383582798E-4	0.0	0.0	16.737183043981094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	135	0.0	45.000004	1
CAACGTT	20	7.035102E-4	45.0	8
GTTTCGA	25	3.8923114E-5	45.0	11
ACGCATA	40	6.8212103E-9	45.0	15
GCGAATC	40	6.8212103E-9	45.0	21
CGCATAC	25	3.8923114E-5	45.0	26
TACGGAC	35	1.2128112E-7	45.0	32
GTTACGT	35	1.2128112E-7	45.0	45
CGTACGA	35	1.2128112E-7	45.0	24
CGTGTAA	20	7.035102E-4	45.0	35
CGTCCTA	45	3.8562575E-10	45.0	23
GATTCGA	20	7.035102E-4	45.0	23
CAATCGA	35	1.2128112E-7	45.0	32
AATACGC	20	7.035102E-4	45.0	15
GTAACGT	25	3.8923114E-5	45.0	24
AATCGAC	35	1.2128112E-7	45.0	33
TACCGGA	20	7.035102E-4	45.0	34
CTTCGAA	30	2.1666747E-6	44.999996	30
AACCGTA	30	2.1666747E-6	44.999996	22
CCTATCG	30	2.1666747E-6	44.999996	37
>>END_MODULE