FastQCFastQC Report
Thu 26 May 2016
SRR1545627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545627_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2459062
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT122670.49884874801855345RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT84530.34374895793599347Illumina PCR Primer Index 11 (96% over 26bp)
TGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC77510.31520148739641374Illumina PCR Primer Index 11 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC75490.3069869730816059RNA PCR Primer, Index 41 (96% over 26bp)
TTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC65370.2658330696826676Illumina PCR Primer Index 11 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC64210.26111582383852056Illumina PCR Primer Index 11 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC56970.23167370322505085RNA PCR Primer, Index 41 (96% over 26bp)
GGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC54450.22142589328776582RNA PCR Primer, Index 41 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC42610.173277452947506RNA PCR Primer, Index 41 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC35910.14603129160631168Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT26610.10821199302823598RNA PCR Primer, Index 41 (96% over 27bp)
TCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC25500.10369807674633662RNA PCR Primer, Index 41 (96% over 26bp)
CTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC24950.10146145156161171RNA PCR Primer, Index 41 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA351.0201984E-746.00000439
TTTCGAA351.0201984E-746.00000439
GCGATAA351.0201984E-746.0000049
TCTATCG351.0201984E-746.00000438
ACGAATC351.0201984E-746.00000431
AACCGTA301.8622759E-646.020
TAATACG253.4182995E-546.01
ACGTTAT301.8622759E-646.036
GCGAAAC405.6152203E-946.044
ATTACGT206.3128653E-446.023
TACGGAT301.8622759E-646.015
ATCGTAC501.6370905E-1146.014
ATTCCGT301.8622759E-646.016
TAACCGT206.3128653E-446.017
CGTAGAA206.3128653E-446.041
CGCTAAG253.4182995E-546.01
AACGCGT253.4182995E-546.042
GTCGTAC206.3128653E-446.036
CGAATTA501.6370905E-1146.039
GCGATGC206.3128653E-446.029
Produced by FastQC (version 0.11.5)