##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545627_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2459062
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54622453602227	33.0	31.0	34.0	31.0	34.0
2	32.9695977571936	34.0	31.0	34.0	31.0	34.0
3	33.03718450368474	34.0	33.0	34.0	31.0	34.0
4	36.41242717751728	37.0	37.0	37.0	35.0	37.0
5	36.39394248701334	37.0	37.0	37.0	35.0	37.0
6	36.39624905756748	37.0	37.0	37.0	35.0	37.0
7	36.48579539678138	37.0	37.0	37.0	35.0	37.0
8	36.52051351287605	37.0	37.0	37.0	35.0	37.0
9	38.34485954400499	39.0	39.0	39.0	37.0	39.0
10	38.09343888035357	39.0	39.0	39.0	35.0	39.0
11	37.94570978690248	39.0	38.0	39.0	35.0	39.0
12	37.622780556163285	39.0	37.0	39.0	35.0	39.0
13	37.589782201506104	39.0	37.0	39.0	35.0	39.0
14	38.91620910737509	41.0	38.0	41.0	35.0	41.0
15	38.967591301073334	41.0	38.0	41.0	35.0	41.0
16	39.02920585166214	41.0	38.0	41.0	35.0	41.0
17	38.98126968738487	41.0	38.0	41.0	35.0	41.0
18	38.968378186479235	41.0	38.0	41.0	35.0	41.0
19	38.948873188231936	41.0	38.0	41.0	35.0	41.0
20	38.861982333101	41.0	38.0	41.0	35.0	41.0
21	38.81417345312969	40.0	38.0	41.0	35.0	41.0
22	38.91560155864309	41.0	38.0	41.0	35.0	41.0
23	38.90448024490639	41.0	38.0	41.0	35.0	41.0
24	38.886842625358774	41.0	38.0	41.0	35.0	41.0
25	38.80925735097367	41.0	38.0	41.0	35.0	41.0
26	38.70029913845198	40.0	38.0	41.0	35.0	41.0
27	38.62607815500382	40.0	38.0	41.0	35.0	41.0
28	38.57364678076437	40.0	37.0	41.0	35.0	41.0
29	38.55142692620194	40.0	37.0	41.0	35.0	41.0
30	38.44332188452345	40.0	37.0	41.0	35.0	41.0
31	38.41208558385271	40.0	37.0	41.0	35.0	41.0
32	38.34633205669479	40.0	37.0	41.0	35.0	41.0
33	38.233319452701885	40.0	36.0	41.0	34.0	41.0
34	38.19646759618098	40.0	36.0	41.0	34.0	41.0
35	38.12253737400684	40.0	36.0	41.0	34.0	41.0
36	38.04328723716604	40.0	36.0	41.0	34.0	41.0
37	37.96320832902953	40.0	36.0	41.0	34.0	41.0
38	37.907134509011975	40.0	35.0	41.0	34.0	41.0
39	37.83989952266352	40.0	35.0	41.0	34.0	41.0
40	37.70600293933215	40.0	35.0	41.0	33.0	41.0
41	37.580794628195626	40.0	35.0	41.0	33.0	41.0
42	37.49012631645725	40.0	35.0	41.0	33.0	41.0
43	37.44981867069639	39.0	35.0	41.0	33.0	41.0
44	37.30062641771537	39.0	35.0	41.0	33.0	41.0
45	37.222131446868765	39.0	35.0	41.0	33.0	41.0
46	37.21370912974134	39.0	35.0	41.0	33.0	41.0
47	37.17423228857182	39.0	35.0	41.0	33.0	41.0
48	37.057321450211504	39.0	35.0	41.0	33.0	41.0
49	37.00215041345033	39.0	35.0	41.0	33.0	41.0
50	36.90331760646946	38.0	35.0	41.0	33.0	41.0
51	36.784776878338164	38.0	35.0	41.0	33.0	41.0
52	36.47084742068317	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	7.0
13	14.0
14	18.0
15	51.0
16	106.0
17	162.0
18	294.0
19	547.0
20	928.0
21	1451.0
22	2202.0
23	3327.0
24	4701.0
25	6344.0
26	8357.0
27	10653.0
28	13665.0
29	17643.0
30	22660.0
31	29460.0
32	39001.0
33	54501.0
34	123044.0
35	234190.0
36	140461.0
37	212106.0
38	371260.0
39	1159922.0
40	1981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.72685763921365	14.144580331850113	47.26936531083803	11.859196718098202
2	25.641931760972277	12.930621513406331	49.65421774644153	11.773228979179866
3	25.509279554561857	13.198853871923522	46.31839294820546	14.973473625309165
4	22.725535183740792	14.447216052299616	47.993950538864006	14.833298225095584
5	22.845255629992252	16.937921858009272	44.63901276177665	15.577809750221833
6	24.510036753851672	18.795093413667487	44.82717393868069	11.867695893800157
7	85.99291111814179	2.439182094636085	6.937767327541964	4.630139459680154
8	87.24826783545922	2.7015178958480917	6.775347673218487	3.2748665954742093
9	79.90384138342182	4.2895624429152255	8.880255967519322	6.926340206143642
10	42.38189195717717	23.205636946120105	14.259664864082321	20.152806232620403
11	31.954257355040255	21.96646526195761	25.00851950865818	21.070757874343958
12	25.90585353276981	23.02451097206984	29.332485313505718	21.73715018165463
13	21.496001320828835	24.423459026246594	34.14924064541683	19.93129900750774
14	16.79209389596521	31.78175255442929	31.034963738205867	20.39118981139963
15	16.768954991781417	22.44937297229594	38.808659562060654	21.973012473861985
16	21.810348824063812	21.242937347655324	33.08167911179141	23.865034716489458
17	20.421485916174543	21.987245543219323	28.336211124404347	29.255057416201787
18	22.17125879705351	22.247832710196004	29.938488740828824	25.64241975192167
19	24.588237303492143	24.462701631760403	27.93313060020447	23.015930464542983
20	26.065833232346318	21.181694483506313	25.6125709721837	27.139901311963666
21	23.888336284323046	24.68225689307549	28.10673338045157	23.322673442149895
22	27.70100957194247	20.874626178599808	25.53437042254323	25.88999382691449
23	26.460861905881185	23.551541197415926	23.491965635677346	26.495631261025544
24	26.62189891918138	20.114824270392532	28.391191438036127	24.87208537238996
25	29.131067049143127	23.030041536162976	23.776261029612105	24.06263038508179
26	22.996126165180055	25.462188427945286	24.656881363706976	26.88480404316768
27	21.69152302788624	22.529566151646442	27.124082272020793	28.65482854844652
28	22.52013165995815	22.836959783852542	27.680839279367497	26.962069276821815
29	23.878779794897405	21.882124159537256	26.700831455245943	27.5382645903194
30	22.140312037679408	26.210563214754245	28.271186330397523	23.377938417168824
31	23.571630158166	25.496957783089652	23.439099949492935	27.492312109251415
32	27.85708534392382	25.228115435885716	22.306432290035794	24.608366930154666
33	23.41819767049387	26.633814031529095	24.643542944423523	25.30444535355351
34	21.05558948900028	23.660769838255398	25.283014417692602	30.00062625505172
35	21.42093204644698	25.291350929744755	27.946753680875062	25.340963342933197
36	24.48331924937232	24.04673001331402	26.389615227269587	25.080335510044073
37	24.859234944055906	26.492743981241627	23.865319377876602	24.782701696825864
38	21.685870466055757	24.713732309311435	28.965678783210834	24.634718441421974
39	21.695833614605895	27.583891744087786	23.419580311517155	27.300694329789167
40	24.27567910040495	22.614720572315786	29.29767529244891	23.811925034830352
41	21.964025307210637	22.944236460894437	27.28682725364387	27.80491097825106
42	21.204508060390506	24.703118506162106	25.28102178798257	28.811351645464818
43	22.1810999478663	21.181531819856513	28.29745650983993	28.339911722437254
44	25.96762505378067	21.44053301624766	25.744897851294517	26.846944078677154
45	20.295340255756056	24.469655502789276	26.440732279218658	28.794271962236007
46	21.330328393509397	23.426859509845624	26.824659158654807	28.418152937990175
47	22.943667138120144	23.523197056438594	29.427399553162953	24.105736252278305
48	20.207054559828098	21.986472890882784	31.11507558573147	26.69139696355765
49	20.573698426473182	22.528712167485	27.724839796637905	29.17274960940391
50	19.731222718255985	23.06704751649206	30.96286307543283	26.238866689819123
51	22.72049261059705	22.328961205532842	27.830083178057325	27.120463005812784
52	20.76120894877803	22.108389296406518	28.292942593558028	28.837459161257424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	396.0
1	322.5
2	249.0
3	214.5
4	180.0
5	230.0
6	280.0
7	466.5
8	653.0
9	834.0
10	1015.0
11	941.5
12	868.0
13	1014.5
14	1246.0
15	1331.0
16	1688.0
17	2045.0
18	2033.5
19	2022.0
20	2811.5
21	3601.0
22	4295.0
23	4989.0
24	6539.0
25	8089.0
26	10182.5
27	12276.0
28	13912.0
29	15548.0
30	18831.5
31	22115.0
32	28859.5
33	35604.0
34	45608.5
35	55613.0
36	59857.5
37	64102.0
38	65514.5
39	78715.5
40	90504.0
41	105825.5
42	121147.0
43	130280.0
44	139413.0
45	164350.5
46	189288.0
47	220587.0
48	251886.0
49	258804.0
50	265722.0
51	237423.0
52	209124.0
53	181889.5
54	154655.0
55	149132.5
56	143610.0
57	138543.0
58	133476.0
59	125531.5
60	117587.0
61	111001.5
62	104416.0
63	90465.0
64	65547.5
65	54581.0
66	47098.5
67	39616.0
68	32423.5
69	25231.0
70	20742.0
71	16253.0
72	14052.5
73	11852.0
74	9461.5
75	7071.0
76	5208.5
77	3346.0
78	2809.5
79	2273.0
80	1722.0
81	1171.0
82	955.0
83	739.0
84	550.5
85	362.0
86	206.0
87	50.0
88	36.5
89	26.0
90	29.0
91	23.0
92	17.0
93	11.5
94	6.0
95	5.0
96	4.0
97	5.0
98	6.0
99	16.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2459062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.678641282599639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.31376749411085	9.587173638611699
2	12.706480401907683	3.730277355679708
3	5.8645452753540495	2.5825066914745984
4	3.3295756561648746	1.9549458672048203
5	2.1812849408926365	1.6009149591249785
6	1.56387491455009	1.377333532892216
7	1.1071045041913072	1.1375552915562168
8	0.8187971117659806	0.9615063269473179
9	0.6386769927103432	0.8437419424300168
>10	3.8134711271412285	11.02145082427712
>50	0.7739630990238744	8.354229290098566
>100	1.8676909769366739	51.89778281881773
>500	0.014952603780365718	1.4422937860386171
>1k	0.003599700910088044	1.0647001754341714
>5k	0.001938300490047408	1.9449932237087766
>10k+	2.769000700067726E-4	0.49859427570341597
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT	12267	0.49884874801855345	RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	8453	0.34374895793599347	Illumina PCR Primer Index 11 (96% over 26bp)
TGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	7751	0.31520148739641374	Illumina PCR Primer Index 11 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	7549	0.3069869730816059	RNA PCR Primer, Index 41 (96% over 26bp)
TTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	6537	0.2658330696826676	Illumina PCR Primer Index 11 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	6421	0.26111582383852056	Illumina PCR Primer Index 11 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	5697	0.23167370322505085	RNA PCR Primer, Index 41 (96% over 26bp)
GGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	5445	0.22142589328776582	RNA PCR Primer, Index 41 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4261	0.173277452947506	RNA PCR Primer, Index 41 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	3591	0.14603129160631168	Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	2661	0.10821199302823598	RNA PCR Primer, Index 41 (96% over 27bp)
TCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2550	0.10369807674633662	RNA PCR Primer, Index 41 (96% over 26bp)
CTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2495	0.10146145156161171	RNA PCR Primer, Index 41 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.514667787961426	0.0
2	0.0	0.0	0.0	1.0987522884742231	0.0
3	0.0	0.0	0.0	3.532119157630023	0.0
4	0.0	0.0	0.0	4.07163381809812	0.0
5	0.0	0.0	0.0	4.9421283399930545	0.0
6	0.0	0.0	0.0	7.698829879035177	0.0
7	0.0	0.0	0.0	8.044530800768749	0.0
8	0.0	0.0	0.0	8.699414654856202	0.0
9	0.0	0.0	0.0	9.114450957316246	0.0
10	0.0	0.0	0.0	10.520068221134725	0.0
11	0.0	0.0	0.0	11.698769693484751	0.0
12	0.0	0.0	0.0	12.23092382379948	0.0
13	0.0	0.0	0.0	12.483906465148094	0.0
14	0.0	0.0	0.0	12.63559031858489	0.0
15	0.0	0.0	0.0	12.870313965243659	0.0
16	0.0	0.0	0.0	13.404135397968819	0.0
17	0.0	0.0	0.0	14.179959675681214	0.0
18	0.0	0.0	0.0	15.000109797963614	0.0
19	0.0	0.0	0.0	15.50591241701104	0.0
20	0.0	0.0	0.0	16.181251225060613	0.0
21	4.066591244954377E-5	0.0	0.0	17.044832541839124	0.0
22	1.6266364979817508E-4	0.0	0.0	17.76811646066671	0.0
23	1.6266364979817508E-4	0.0	0.0	18.545852036264233	0.0
24	1.6266364979817508E-4	0.0	0.0	19.11582546515704	0.0
25	2.0332956224771885E-4	0.0	0.0	19.600034484693758	0.0
26	2.0332956224771885E-4	0.0	0.0	20.04386225316808	0.0
27	2.0332956224771885E-4	0.0	0.0	20.48927599222793	0.0
28	2.4399547469726262E-4	0.0	0.0	20.96787311584661	0.0
29	2.4399547469726262E-4	0.0	0.0	21.462126615758365	0.0
30	2.4399547469726262E-4	0.0	0.0	21.984561592997654	0.0
31	2.4399547469726262E-4	0.0	0.0	22.461044089168958	0.0
32	2.4399547469726262E-4	0.0	0.0	22.925936800292146	0.0
33	2.4399547469726262E-4	0.0	0.0	23.43007211692914	0.0
34	2.4399547469726262E-4	0.0	0.0	23.9103772088707	0.0
35	2.4399547469726262E-4	0.0	0.0	24.459936349713836	0.0
36	3.2532729959635015E-4	0.0	0.0	24.966267625623104	0.0
37	3.2532729959635015E-4	0.0	0.0	25.418147244762434	0.0
38	3.2532729959635015E-4	0.0	0.0	25.910977437738456	0.0
39	3.2532729959635015E-4	0.0	0.0	26.49010069693241	0.0
40	3.659932120458939E-4	0.0	0.0	26.9943986772192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	35	1.0201984E-7	46.000004	39
TTTCGAA	35	1.0201984E-7	46.000004	39
GCGATAA	35	1.0201984E-7	46.000004	9
TCTATCG	35	1.0201984E-7	46.000004	38
ACGAATC	35	1.0201984E-7	46.000004	31
AACCGTA	30	1.8622759E-6	46.0	20
TAATACG	25	3.4182995E-5	46.0	1
ACGTTAT	30	1.8622759E-6	46.0	36
GCGAAAC	40	5.6152203E-9	46.0	44
ATTACGT	20	6.3128653E-4	46.0	23
TACGGAT	30	1.8622759E-6	46.0	15
ATCGTAC	50	1.6370905E-11	46.0	14
ATTCCGT	30	1.8622759E-6	46.0	16
TAACCGT	20	6.3128653E-4	46.0	17
CGTAGAA	20	6.3128653E-4	46.0	41
CGCTAAG	25	3.4182995E-5	46.0	1
AACGCGT	25	3.4182995E-5	46.0	42
GTCGTAC	20	6.3128653E-4	46.0	36
CGAATTA	50	1.6370905E-11	46.0	39
GCGATGC	20	6.3128653E-4	46.0	29
>>END_MODULE