FastQCFastQC Report
Thu 26 May 2016
SRR1545626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545626_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1300804
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC70590.542664382950852No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.304427108157724No Hit
ATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG34440.2647593334583842No Hit
ATGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT29560.22724407366521016No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT29330.22547593642085972No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26740.20556517353882675No Hit
ATTGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC24350.18719192130405504No Hit
ATATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC23400.17988874572956418No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG22400.17220119249325802No Hit
ATGCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC21190.16289925307732758No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC19420.14929228384906565No Hit
ATGTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC19340.14867727959016117No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC17250.13261029332628127No Hit
ACGCTCTGGGGCACTAGCATTATGTATGTGACATATTTCTTTACCAATTTT17040.130995907146657No Hit
ATGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC14940.11485204535041406No Hit
ATTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC14260.10962450914972585No Hit
ATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC13200.10147570271924133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA650.045.0000042
GCGACTA551.8189894E-1245.00000411
ACGTTAG650.045.0000043
GATCATA650.045.00000424
CGTCTAG650.045.0000044
TAACGAA650.045.0000042
ATCGTAA551.8189894E-1245.0000041
CGTTAGC551.8189894E-1245.00000442
TGCGCGA551.8189894E-1245.0000042
GTCAATC551.8189894E-1245.00000439
GACGGAG551.8189894E-1245.0000043
TCGTACT650.045.00000444
TCATCGG2600.045.00000444
ATCGGCC650.045.0000041
CGACTAA551.8189894E-1245.00000412
ATTGAGC551.8189894E-1245.0000041
GGCTACG650.045.0000043
TGATCGA351.2121018E-745.028
TCTGCGA207.033629E-445.023
AACGTTC253.8910912E-545.014