##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545626_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1300804
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94831119830505	31.0	31.0	34.0	31.0	34.0
2	32.60063468439519	34.0	31.0	34.0	31.0	34.0
3	32.529376447181896	34.0	31.0	34.0	31.0	34.0
4	35.99846325810806	37.0	35.0	37.0	35.0	37.0
5	35.76362541935603	37.0	35.0	37.0	35.0	37.0
6	35.7525276675041	37.0	35.0	37.0	35.0	37.0
7	35.72624853552111	37.0	35.0	37.0	33.0	37.0
8	35.6920373861089	37.0	35.0	37.0	33.0	37.0
9	37.91479654121605	39.0	38.0	39.0	35.0	39.0
10	37.87004806258283	39.0	38.0	39.0	35.0	39.0
11	37.707959077616614	39.0	38.0	39.0	35.0	39.0
12	37.16398781061559	39.0	37.0	39.0	33.0	39.0
13	36.872461954299034	39.0	37.0	39.0	33.0	39.0
14	37.314224894757395	39.0	35.0	41.0	33.0	41.0
15	37.03249375001922	39.0	35.0	41.0	31.0	41.0
16	37.13636643183754	39.0	35.0	41.0	32.0	41.0
17	37.28784659333766	39.0	35.0	41.0	32.0	41.0
18	37.41456591461896	39.0	35.0	41.0	33.0	41.0
19	37.41775932423332	39.0	35.0	41.0	33.0	41.0
20	37.37196226333867	39.0	35.0	41.0	32.0	41.0
21	37.344715268403235	39.0	35.0	41.0	32.0	41.0
22	37.24619619865867	39.0	35.0	41.0	32.0	41.0
23	37.19542298455417	39.0	35.0	41.0	32.0	41.0
24	37.165475352166816	39.0	35.0	41.0	32.0	41.0
25	37.12667242720656	39.0	35.0	41.0	32.0	41.0
26	37.02294350263376	39.0	35.0	41.0	32.0	41.0
27	36.960135423937814	39.0	35.0	41.0	32.0	41.0
28	36.86951147136694	39.0	35.0	41.0	32.0	41.0
29	36.76536050012146	38.0	35.0	41.0	31.0	41.0
30	36.76484697156528	38.0	35.0	41.0	31.0	41.0
31	36.702014292698976	38.0	35.0	41.0	31.0	41.0
32	36.60197616243492	38.0	35.0	41.0	31.0	41.0
33	36.49843250789512	38.0	35.0	41.0	31.0	41.0
34	36.44357105297954	38.0	35.0	41.0	31.0	41.0
35	36.316935526028516	38.0	35.0	41.0	30.0	41.0
36	36.253756907266585	38.0	35.0	41.0	30.0	41.0
37	36.13331524195805	38.0	35.0	41.0	30.0	41.0
38	36.00590327213016	38.0	35.0	40.0	30.0	41.0
39	35.98062813459983	38.0	35.0	40.0	30.0	41.0
40	35.92583817392936	38.0	35.0	40.0	30.0	41.0
41	35.915203212782245	38.0	35.0	40.0	30.0	41.0
42	35.77493150390067	38.0	35.0	40.0	29.0	41.0
43	35.700924966405395	38.0	35.0	40.0	29.0	41.0
44	35.61561080685484	37.0	35.0	40.0	29.0	41.0
45	35.599537670548365	37.0	35.0	40.0	29.0	41.0
46	35.50205334546942	37.0	34.0	40.0	29.0	41.0
47	35.413769484103675	37.0	34.0	40.0	28.0	41.0
48	35.39307843456816	37.0	34.0	40.0	28.0	41.0
49	35.36189156859911	37.0	34.0	40.0	29.0	41.0
50	35.29077478236537	37.0	34.0	40.0	28.0	41.0
51	35.17274239624109	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	17.0
11	10.0
12	9.0
13	16.0
14	18.0
15	24.0
16	61.0
17	167.0
18	346.0
19	692.0
20	1189.0
21	1933.0
22	3118.0
23	4550.0
24	6855.0
25	9942.0
26	13155.0
27	15752.0
28	18062.0
29	21396.0
30	26432.0
31	33070.0
32	41424.0
33	53365.0
34	88598.0
35	200181.0
36	85679.0
37	115574.0
38	173038.0
39	385863.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.020135239436533	91.71719951660666	2.6558190165466895	3.606846227410125
2	3.5235900258609294	2.3660751350703104	91.38040780932408	2.729927029744681
3	24.53597928665656	16.79645819047297	44.4407458771652	14.22681664570527
4	25.649675124000233	15.878103080863834	44.80605840695447	13.666163388181463
5	23.90144864253185	14.413854815944601	46.80305411114972	14.881642430373832
6	20.148923281293722	14.27102007681403	50.385453919268386	15.194602722623854
7	21.908142963890025	16.493337966365416	47.38338750495848	14.215131564786084
8	24.794434826461174	19.232797562123118	43.636474057582845	12.33629355383286
9	82.53172653220624	3.566332821854791	9.77134141653931	4.130599229399664
10	83.73190734345836	3.797728174267607	8.970298369316207	3.5000661129578323
11	78.16289002801344	5.8987364737500805	9.735978671652301	6.202394826584174
12	42.80483454847925	24.636839985116897	15.479042192367181	17.079283274036673
13	36.05708469531152	22.724176739923923	25.502227852927884	15.716510711836678
14	33.28679801107622	24.091715585130427	25.844938976202407	16.776547427590934
15	20.214267483802324	35.13365580056642	29.657734754813177	14.994341960818078
16	14.999338870421678	39.02255835621662	28.88398252157896	17.094120251782744
17	12.565920769001327	25.06042416843737	43.24748386382576	19.126171198735552
18	15.001491385327844	21.458651726163204	40.90900704487378	22.63084984363517
19	16.872641843044764	20.72410601443415	30.341850117312063	32.06140202520903
20	19.404460625889836	23.213566378947174	35.26911048859013	22.11286250657286
21	23.708567931832928	25.503534736978057	28.840624721326197	21.94727260986282
22	26.15228735458993	22.021226871996088	28.571637233587843	23.25484853982614
23	21.092877943179754	27.215860344832887	29.00567648930969	22.685585222677666
24	25.87015415081749	23.655062561308238	24.07795486483744	26.396828423036826
25	22.072425976549887	27.969163686458533	23.763072684278338	26.195337652713246
26	21.849410057164643	21.732559247972794	36.14995033840609	20.26808035645647
27	22.94381013588519	25.779748524758535	30.148815655548418	21.12762568380786
28	19.773155679103077	30.094080276505913	25.55642510324384	24.576338941147167
29	17.5620616172767	29.26782205466773	27.400669124633687	25.769447203421887
30	18.070516388325988	26.84232213308077	31.617753327941795	23.469408150651443
31	16.015018404002447	23.94342268320208	31.066248258769193	28.97531065402628
32	16.749794742328593	31.096921596182053	30.710237668395852	21.443045993093502
33	23.328495299829953	29.94125171816815	26.78243609336995	19.94781688863195
34	24.02429574324802	26.959249817804988	25.154596695582114	23.861857743364872
35	23.132693318901236	29.306182945316895	25.079181798333956	22.481941937447917
36	17.310755501981852	25.918124483012043	28.013290242034927	28.757829772971178
37	19.40192373332185	25.914280706393892	30.894354568405387	23.78944099187887
38	24.63714748724635	28.179725769600957	24.925507609140194	22.2576191340125
39	19.32197317966427	30.22607556557329	29.896510158332845	20.555441096429593
40	18.133400573798973	28.90028013443993	31.260743355647737	21.70557593611336
41	17.988413319762238	32.66080055104382	25.302428344316287	24.04835778487766
42	22.681433943930063	26.725394448356553	27.320026691184836	23.273144916528548
43	18.26416585434854	25.635299399448343	28.65527781279885	27.445256933404266
44	18.32159187702375	25.486698995390544	27.70509623279141	28.486612894794295
45	19.612408940931918	24.242007250900212	27.675268526234543	28.470315281933328
46	20.124399986469907	27.093705123907984	27.79088932690859	24.99100556271352
47	16.184375201798275	31.161035790172846	26.04051033053404	26.61407867749484
48	18.80775274368775	30.113760412790857	27.163584982826006	23.914901860695384
49	19.000864080983764	27.52466935833531	29.513900633761892	23.960565926919045
50	18.9857964766406	24.57510893262936	33.18301604238609	23.256078548343947
51	19.496634389193147	23.835873813426158	30.075860775335865	26.591631022044826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1031.0
1	1227.0
2	1423.0
3	3645.0
4	5867.0
5	3995.0
6	2123.0
7	2312.0
8	2501.0
9	2689.0
10	2877.0
11	3057.0
12	3237.0
13	3259.5
14	3282.0
15	3413.5
16	3545.0
17	3487.0
18	3429.0
19	3740.0
20	4051.0
21	3524.0
22	2997.0
23	4052.0
24	5107.0
25	7257.0
26	13144.5
27	16882.0
28	16963.0
29	17044.0
30	21096.0
31	25148.0
32	31758.5
33	38369.0
34	45245.5
35	52122.0
36	57270.0
37	62418.0
38	75456.0
39	88494.0
40	103215.5
41	117937.0
42	123193.0
43	128449.0
44	139328.0
45	150207.0
46	144250.0
47	138293.0
48	116927.5
49	95562.0
50	83900.5
51	72239.0
52	65900.5
53	59562.0
54	52302.5
55	45043.0
56	41953.5
57	38864.0
58	35321.0
59	31778.0
60	28975.0
61	26172.0
62	21727.0
63	17282.0
64	13387.5
65	9493.0
66	9011.0
67	8529.0
68	6246.5
69	3964.0
70	3228.5
71	2493.0
72	1892.0
73	1291.0
74	986.5
75	792.0
76	902.0
77	700.0
78	498.0
79	337.0
80	176.0
81	100.5
82	25.0
83	16.0
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1300804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.163608626467747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63417880738206	10.559054344606162
2	11.722123185886415	3.554993765240497
3	5.423680237373594	2.4672769330392263
4	3.145746185265312	1.9080345596626838
5	1.9474333274621711	1.4765058401888085
6	1.291911126530487	1.1754020821732416
7	0.936629224154744	0.9941875288236269
8	0.6768117023480836	0.8210326214575778
9	0.4792843893702998	0.6540912811068125
>10	2.8098771474065454	8.142679474480557
>50	0.44693916160948355	4.901358929507949
>100	1.4136105373441945	54.16658497074496
>500	0.06057603671010413	5.355385721266749
>1k	0.010689888831194845	3.2785334840744285
>5k	5.090423252949927E-4	0.544878463626722
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	7059	0.542664382950852	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3960	0.304427108157724	No Hit
ATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	3444	0.2647593334583842	No Hit
ATGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	2956	0.22724407366521016	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2933	0.22547593642085972	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2674	0.20556517353882675	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	2435	0.18719192130405504	No Hit
ATATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	2340	0.17988874572956418	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	2240	0.17220119249325802	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	2119	0.16289925307732758	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1942	0.14929228384906565	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1934	0.14867727959016117	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1725	0.13261029332628127	No Hit
ACGCTCTGGGGCACTAGCATTATGTATGTGACATATTTCTTTACCAATTTT	1704	0.130995907146657	No Hit
ATGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1494	0.11485204535041406	No Hit
ATTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1426	0.10962450914972585	No Hit
ATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1320	0.10147570271924133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.687553236306162E-5	0.0	0.0	0.2423116780083702	0.0
2	7.687553236306162E-5	0.0	0.0	1.0036869505321324	0.0
3	7.687553236306162E-5	0.0	0.0	1.3745345186515416	0.0
4	7.687553236306162E-5	0.0	0.0	1.8473959182167337	0.0
5	7.687553236306162E-5	0.0	0.0	3.468316518091888	0.0
6	7.687553236306162E-5	0.0	0.0	3.8377034510963988	0.0
7	7.687553236306162E-5	0.0	0.0	4.42303375450875	0.0
8	1.5375106472612324E-4	0.0	0.0	6.349765222124163	0.0
9	1.5375106472612324E-4	0.0	0.0	6.583620591572597	0.0
10	1.5375106472612324E-4	0.0	0.0	7.0389543697590105	0.0
11	2.3062659708918484E-4	0.0	0.0	7.372056051488157	0.0
12	2.3062659708918484E-4	0.0	0.0	8.384276186112588	0.0
13	3.075021294522465E-4	0.0	0.0	9.274110473215027	0.0
14	3.075021294522465E-4	0.0	0.0	9.667251945719723	0.0
15	3.8437766181530805E-4	0.0	0.0	9.861055162807002	0.0
16	3.8437766181530805E-4	0.0	0.0	9.969449663438919	0.0
17	3.8437766181530805E-4	0.0	0.0	10.145648383615056	0.0
18	3.8437766181530805E-4	0.0	0.0	10.560622507310864	0.0
19	3.8437766181530805E-4	0.0	0.0	11.147797823499928	0.0
20	4.612531941783697E-4	0.0	0.0	11.823995006165418	0.0
21	5.381287265414313E-4	0.0	0.0	12.183772497624545	0.0
22	5.381287265414313E-4	0.0	0.0	12.61927238846129	0.0
23	6.15004258904493E-4	0.0	0.0	13.261798087951759	0.0
24	6.15004258904493E-4	0.0	0.0	13.780861682467151	0.0
25	7.687553236306161E-4	0.0	0.0	14.38095208809321	0.0
26	7.687553236306161E-4	0.0	0.0	14.839899016300688	0.0
27	7.687553236306161E-4	0.0	0.0	15.229888591978499	0.0
28	8.456308559936777E-4	0.0	0.0	15.547077038508492	0.0
29	8.456308559936777E-4	0.0	0.0	15.87018490103044	0.0
30	8.456308559936777E-4	0.0	0.0	16.207284110442465	0.0
31	9.225063883567394E-4	0.0	0.0	16.590816141401778	0.0
32	9.225063883567394E-4	0.0	0.0	17.028699173741778	0.0
33	9.225063883567394E-4	0.0	0.0	17.386477901359466	0.0
34	9.225063883567394E-4	0.0	0.0	17.739336594905918	0.0
35	9.225063883567394E-4	0.0	0.0	18.07474454260596	0.0
36	9.225063883567394E-4	0.0	0.0	18.42737260955532	0.0
37	0.002229390438528787	0.0	0.0	18.92175915818217	0.0
38	0.002229390438528787	0.0	0.0	19.28760981669798	0.0
39	0.002229390438528787	0.0	0.0	19.65845738481739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCA	65	0.0	45.000004	2
GCGACTA	55	1.8189894E-12	45.000004	11
ACGTTAG	65	0.0	45.000004	3
GATCATA	65	0.0	45.000004	24
CGTCTAG	65	0.0	45.000004	4
TAACGAA	65	0.0	45.000004	2
ATCGTAA	55	1.8189894E-12	45.000004	1
CGTTAGC	55	1.8189894E-12	45.000004	42
TGCGCGA	55	1.8189894E-12	45.000004	2
GTCAATC	55	1.8189894E-12	45.000004	39
GACGGAG	55	1.8189894E-12	45.000004	3
TCGTACT	65	0.0	45.000004	44
TCATCGG	260	0.0	45.000004	44
ATCGGCC	65	0.0	45.000004	1
CGACTAA	55	1.8189894E-12	45.000004	12
ATTGAGC	55	1.8189894E-12	45.000004	1
GGCTACG	65	0.0	45.000004	3
TGATCGA	35	1.2121018E-7	45.0	28
TCTGCGA	20	7.033629E-4	45.0	23
AACGTTC	25	3.8910912E-5	45.0	14
>>END_MODULE