FastQCFastQC Report
Thu 26 May 2016
SRR1545625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545625_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1083995
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT62530.5768476791867121Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT46080.4250942116891683No Hit
TGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC42780.39465126684163676No Hit
TTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC39970.36872863804722344No Hit
GTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC39270.36227104368562585No Hit
ATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC35950.33164359614204864No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC32510.2999091324221975No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC26180.24151402912375056No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC17850.16466865622073903No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC17270.15931807803541528No Hit
CTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC16290.15027744592917863No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT14620.1348714708093672No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC13690.12629209544324466No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT11180.1031370070895161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC351.019489E-746.00000420
TCACGCT405.6115823E-946.00000438
TCACGCA405.6115823E-946.00000439
AAGTATC351.019489E-746.00000422
TATCACG351.019489E-746.00000424
GTACCTA206.311293E-446.00000440
AACCGAG206.311293E-446.00000411
TCGTTCC405.6115823E-946.00000430
TCCATCG351.019489E-746.00000423
GCGACTT206.311293E-446.0000049
CTCGTGA206.311293E-446.00000416
CTCGTCT351.019489E-746.00000425
CGAGTTT206.311293E-446.00000414
GTCGACG351.019489E-746.00000415
GTCGACC206.311293E-446.00000440
CGACGTG351.019489E-746.00000412
CCGATTA405.6115823E-946.00000411
CACGGGT405.6115823E-946.00000414
CACGGAC206.311293E-446.00000437
ACGCATA206.311293E-446.00000410
Produced by FastQC (version 0.11.5)