##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545625_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1083995
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53962518277298	33.0	31.0	34.0	31.0	34.0
2	32.94864090701525	34.0	31.0	34.0	31.0	34.0
3	33.0203100567807	34.0	33.0	34.0	31.0	34.0
4	36.39472414540658	37.0	37.0	37.0	35.0	37.0
5	36.35864464319485	37.0	37.0	37.0	35.0	37.0
6	36.36716959026564	37.0	37.0	37.0	35.0	37.0
7	36.46342649181961	37.0	37.0	37.0	35.0	37.0
8	36.51214166117003	37.0	37.0	37.0	35.0	37.0
9	38.341663937564284	39.0	39.0	39.0	37.0	39.0
10	38.060205997260134	39.0	39.0	39.0	35.0	39.0
11	37.86491173852278	39.0	38.0	39.0	35.0	39.0
12	37.34668056586977	39.0	35.0	39.0	35.0	39.0
13	37.17217514840936	39.0	35.0	39.0	35.0	39.0
14	38.473974510952544	40.0	36.0	41.0	35.0	41.0
15	38.526414789736116	40.0	36.0	41.0	35.0	41.0
16	38.63621972426072	40.0	36.0	41.0	35.0	41.0
17	38.618258386800676	40.0	36.0	41.0	35.0	41.0
18	38.6309392570999	40.0	36.0	41.0	35.0	41.0
19	38.59066508609357	40.0	36.0	41.0	35.0	41.0
20	38.49225688310371	40.0	36.0	41.0	35.0	41.0
21	38.4591534093792	40.0	36.0	41.0	35.0	41.0
22	38.53999234313812	40.0	36.0	41.0	35.0	41.0
23	38.5569158529329	40.0	36.0	41.0	35.0	41.0
24	38.513913809565544	40.0	35.0	41.0	35.0	41.0
25	38.459196767512765	40.0	35.0	41.0	35.0	41.0
26	38.347963782120765	40.0	35.0	41.0	35.0	41.0
27	38.24813490837135	40.0	35.0	41.0	35.0	41.0
28	38.261487368484175	40.0	36.0	41.0	35.0	41.0
29	38.221933680505906	40.0	36.0	41.0	35.0	41.0
30	38.18980161347608	40.0	36.0	41.0	35.0	41.0
31	38.124185074654406	40.0	35.0	41.0	35.0	41.0
32	38.10079290033626	40.0	35.0	41.0	35.0	41.0
33	38.01183953800525	40.0	35.0	41.0	34.0	41.0
34	37.950916747770975	40.0	35.0	41.0	34.0	41.0
35	37.89296998602392	40.0	35.0	41.0	34.0	41.0
36	37.78040489116647	40.0	35.0	41.0	34.0	41.0
37	37.73367220328507	40.0	35.0	41.0	34.0	41.0
38	37.66323184147528	40.0	35.0	41.0	34.0	41.0
39	37.64459799168815	40.0	35.0	41.0	34.0	41.0
40	37.50376062620215	40.0	35.0	41.0	33.0	41.0
41	37.395950165821795	39.0	35.0	41.0	33.0	41.0
42	37.37141499730165	39.0	35.0	41.0	33.0	41.0
43	37.32679947785737	39.0	35.0	41.0	33.0	41.0
44	37.19850091559463	39.0	35.0	41.0	33.0	41.0
45	37.10834367317192	39.0	35.0	41.0	33.0	41.0
46	37.127592839450365	39.0	35.0	41.0	33.0	41.0
47	37.09495984760078	39.0	35.0	41.0	33.0	41.0
48	37.0333977555247	39.0	35.0	41.0	33.0	41.0
49	37.02256191218594	39.0	35.0	41.0	33.0	41.0
50	36.90784274835216	38.0	35.0	41.0	33.0	41.0
51	36.80342160249817	38.0	35.0	40.0	33.0	41.0
52	36.473788163229536	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	3.0
13	1.0
14	5.0
15	9.0
16	26.0
17	58.0
18	111.0
19	247.0
20	446.0
21	782.0
22	1144.0
23	1773.0
24	2464.0
25	3130.0
26	3826.0
27	4768.0
28	6116.0
29	7884.0
30	10460.0
31	13365.0
32	17911.0
33	24454.0
34	58290.0
35	160866.0
36	56286.0
37	74366.0
38	136971.0
39	497424.0
40	805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.106661008583988	14.84822346966545	47.78435324886185	13.260762272888712
2	24.350389070060285	13.357626188312677	50.162500749542204	12.129483992084834
3	24.939875183926123	14.257907093667407	45.25352976720372	15.548687955202745
4	20.862273349969325	14.263257671852731	50.04137472958824	14.833094248589706
5	22.74466210637503	16.249798200176198	46.497354692595444	14.508185000853324
6	23.527045788956592	18.696027195697397	44.52511312321551	13.251813892130498
7	84.74660860981831	2.3647710552170444	7.726603904999561	5.162016429965083
8	86.76525260725373	2.4579449167200953	7.305476501275375	3.471325974750806
9	79.62324549467479	3.682581561723071	9.367847637673606	7.326325305928533
10	42.855271472654394	24.02077500357474	13.986688130480307	19.13726539329056
11	35.15108464522438	20.892993048860927	27.519499628688326	16.43642267722637
12	33.14120452585114	23.561824547161194	25.685635081342628	17.611335845645044
13	20.582475011416104	32.982808961295945	31.662138663001215	14.772577364286734
14	15.023962287648928	39.250273294618516	28.440629338696212	17.285135079036344
15	12.378931637138548	24.728804099649906	41.938754330047644	20.9535099331639
16	15.22663849925507	22.58820382012832	39.0052537142699	23.179903966346707
17	16.61419102486635	21.78137353032071	28.45871060290868	33.14572484190425
18	18.936065203252785	24.02501856558379	33.0376062620215	24.001309969141925
19	22.42122888020701	26.918574347667658	28.503083501307664	22.157113270817668
20	26.0743822619108	21.243548171347655	27.393392035941126	25.28867753080042
21	20.62417262072242	28.451330495066856	28.86895234756618	22.055544536644543
22	25.181481464397898	23.735349332792126	23.87437211426252	27.208797088547453
23	22.244475297395283	28.53223492728287	21.753329120521776	27.46996065480007
24	22.182205637479875	20.903601953883552	34.92211679943173	21.99207560920484
25	24.550389992573766	25.23932305960821	27.43831844242824	22.771968505389786
26	19.530809643955923	30.133810580307102	24.578434402372704	25.75694537336427
27	18.27591455680146	27.579924261643274	24.3678245748366	29.77633660671867
28	20.332473858274255	24.76819542525565	30.300785520228413	24.59854519624168
29	16.49786207500957	21.7627387580201	29.029838698517985	32.709560468452345
30	16.928399116232086	29.769694509661022	29.987776696386977	23.314129677719915
31	23.28673102735714	31.199774906710825	23.746696248598933	21.766797817333106
32	23.267634998316414	28.043302782761913	22.693185854178292	25.99587636474338
33	22.12897660967071	30.111301251389538	25.159710146264512	22.600011992675242
34	15.601455726271801	27.405753716576182	27.491086213497297	29.501704343654723
35	18.966969404840427	26.286560362363293	30.103736640851665	24.642733591944612
36	24.06690067758615	27.29154654772393	24.556939838283387	24.084612936406533
37	19.983487008703914	28.692660021494564	29.954012703010623	21.3698402667909
38	16.51483632304577	28.47347081859234	32.89194138349347	22.119751474868426
39	18.00792439079516	32.98207095051177	25.041074912707163	23.96892974598591
40	23.302413756521016	26.895972767402064	27.31377912259743	22.487834353479492
41	18.224346053256703	24.877882278054788	29.220153229489064	27.677618439199442
42	18.738370564439872	25.722074363811643	26.114603849648756	29.424951222099732
43	19.79012818324808	22.62538111338152	28.364337473881335	29.220153229489064
44	23.50047740072602	24.830280582475012	25.26220139391787	26.4070406228811
45	16.509670247556492	29.057790856968897	25.695505975581067	28.73703291989354
46	19.105161924178617	29.01932204484338	25.881207939151015	25.99430809182699
47	19.627950313423955	26.47263133132533	29.839713282810344	24.05970507244037
48	18.251744703619483	22.96228303636087	35.18881544656571	23.59715681345394
49	19.46770972190831	22.01237090576986	28.707420237178216	29.81249913514361
50	17.246205010170712	26.309254193976912	32.58474439457746	23.859796401274913
51	19.257007643024185	26.199106084437656	28.330665731853006	26.21322054068515
52	17.410504660999358	23.528337307828913	30.835197579324632	28.225960451847104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	561.0
1	431.5
2	302.0
3	224.5
4	147.0
5	165.0
6	183.0
7	320.0
8	457.0
9	569.5
10	682.0
11	765.0
12	848.0
13	890.5
14	881.0
15	829.0
16	1250.0
17	1671.0
18	1495.5
19	1320.0
20	1377.0
21	1434.0
22	1680.0
23	1926.0
24	2449.5
25	2973.0
26	5152.0
27	7331.0
28	8015.0
29	8699.0
30	10249.0
31	11799.0
32	16400.5
33	21002.0
34	25589.5
35	30177.0
36	34898.5
37	39620.0
38	39378.5
39	51625.0
40	64113.0
41	73420.5
42	82728.0
43	98161.0
44	113594.0
45	123647.0
46	133700.0
47	129924.5
48	126149.0
49	110247.0
50	94345.0
51	81661.5
52	68978.0
53	59362.0
54	49746.0
55	46860.5
56	43975.0
57	38472.0
58	32969.0
59	30395.5
60	27822.0
61	25738.0
62	23654.0
63	20922.5
64	14368.5
65	10546.0
66	9029.0
67	7512.0
68	6614.0
69	5716.0
70	4565.5
71	3415.0
72	2651.5
73	1888.0
74	1452.0
75	1016.0
76	725.0
77	434.0
78	474.5
79	515.0
80	469.5
81	424.0
82	410.5
83	397.0
84	244.0
85	91.0
86	66.0
87	41.0
88	21.5
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1083995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.264555099682383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.47627749195493	9.994662454980396
2	12.344294180015352	3.768603173550658
3	5.8171204635863685	2.6638726751371213
4	3.4679450878669624	2.1174655550567243
5	2.326764989358165	1.7758516192034803
6	1.630092295044771	1.4929580193167173
7	1.1560714750016243	1.2352841710533364
8	0.8902322337643305	1.087119918704716
9	0.6793673807844337	0.9333216735219801
>10	4.03102618146168	11.7085117547005
>50	0.573642118107934	6.316332647096987
>100	1.5811464711139565	51.24705963854099
>500	0.01694301614664783	1.715439947382905
>1k	0.008471508073323916	3.365945892880704
>5k	6.051077195231367E-4	0.5775708588727818
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	6253	0.5768476791867121	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	4608	0.4250942116891683	No Hit
TGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	4278	0.39465126684163676	No Hit
TTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3997	0.36872863804722344	No Hit
GTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3927	0.36227104368562585	No Hit
ATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3595	0.33164359614204864	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3251	0.2999091324221975	No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2618	0.24151402912375056	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1785	0.16466865622073903	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1727	0.15931807803541528	No Hit
CTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1629	0.15027744592917863	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1462	0.1348714708093672	No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1369	0.12629209544324466	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1118	0.1031370070895161	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5959437082274365	0.0
2	0.0	0.0	0.0	1.3237145927794869	0.0
3	0.0	0.0	0.0	4.367086564052417	0.0
4	0.0	0.0	0.0	5.0158902946969315	0.0
5	0.0	0.0	0.0	6.0670944054169995	0.0
6	0.0	0.0	0.0	9.550505306758795	0.0
7	0.0	0.0	0.0	9.984824653250246	0.0
8	0.0	0.0	0.0	10.829293493051168	0.0
9	9.225134802282298E-5	0.0	0.0	11.377266500306735	0.0
10	9.225134802282298E-5	0.0	0.0	13.201260153413992	0.0
11	9.225134802282298E-5	0.0	0.0	14.711506971895627	0.0
12	1.8450269604564597E-4	0.0	0.0	15.404775852287141	0.0
13	2.7675404406846897E-4	0.0	0.0	15.706530011669795	0.0
14	2.7675404406846897E-4	0.0	0.0	15.85274839828597	0.0
15	2.7675404406846897E-4	0.0	0.0	16.098874994810863	0.0
16	2.7675404406846897E-4	0.0	0.0	16.766867005844123	0.0
17	2.7675404406846897E-4	0.0	0.0	17.753126167556122	0.0
18	2.7675404406846897E-4	0.0	0.0	18.882467170051523	0.0
19	2.7675404406846897E-4	0.0	0.0	19.47389056222584	0.0
20	2.7675404406846897E-4	0.0	0.0	20.200093173861504	0.0
21	2.7675404406846897E-4	0.0	0.0	21.230909736668526	0.0
22	2.7675404406846897E-4	0.0	0.0	22.025193843145033	0.0
23	2.7675404406846897E-4	0.0	0.0	22.928888048376606	0.0
24	3.6900539209129194E-4	0.0	0.0	23.582950105858423	0.0
25	3.6900539209129194E-4	0.0	0.0	24.17492700612088	0.0
26	3.6900539209129194E-4	0.0	0.0	24.65694029954013	0.0
27	3.6900539209129194E-4	0.0	0.0	25.1220715962712	0.0
28	3.6900539209129194E-4	0.0	0.0	25.59190771175144	0.0
29	3.6900539209129194E-4	0.0	0.0	26.11709463604537	0.0
30	3.6900539209129194E-4	0.0	0.0	26.725400024907863	0.0
31	3.6900539209129194E-4	0.0	0.0	27.213870912688712	0.0
32	3.6900539209129194E-4	0.0	0.0	27.69385467645146	0.0
33	3.6900539209129194E-4	0.0	0.0	28.13306334438812	0.0
34	3.6900539209129194E-4	0.0	0.0	28.595242597982462	0.0
35	0.0026752890926618664	0.0	0.0	29.252164447252987	0.0
36	0.0026752890926618664	0.0	0.0	29.738144548637216	0.0
37	0.0026752890926618664	0.0	0.0	30.214438258479053	0.0
38	0.0026752890926618664	0.0	0.0	30.686488406311838	0.0
39	0.0027675404406846895	0.0	0.0	31.14276357363272	0.0
40	0.0027675404406846895	0.0	0.0	31.58593904953436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAC	35	1.019489E-7	46.000004	20
TCACGCT	40	5.6115823E-9	46.000004	38
TCACGCA	40	5.6115823E-9	46.000004	39
AAGTATC	35	1.019489E-7	46.000004	22
TATCACG	35	1.019489E-7	46.000004	24
GTACCTA	20	6.311293E-4	46.000004	40
AACCGAG	20	6.311293E-4	46.000004	11
TCGTTCC	40	5.6115823E-9	46.000004	30
TCCATCG	35	1.019489E-7	46.000004	23
GCGACTT	20	6.311293E-4	46.000004	9
CTCGTGA	20	6.311293E-4	46.000004	16
CTCGTCT	35	1.019489E-7	46.000004	25
CGAGTTT	20	6.311293E-4	46.000004	14
GTCGACG	35	1.019489E-7	46.000004	15
GTCGACC	20	6.311293E-4	46.000004	40
CGACGTG	35	1.019489E-7	46.000004	12
CCGATTA	40	5.6115823E-9	46.000004	11
CACGGGT	40	5.6115823E-9	46.000004	14
CACGGAC	20	6.311293E-4	46.000004	37
ACGCATA	20	6.311293E-4	46.000004	10
>>END_MODULE