FastQCFastQC Report
Thu 26 May 2016
SRR1545624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545624_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1362724
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC47700.3500341962128795TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.22513729852853548No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG29440.21603787707562208TruSeq Adapter, Index 20 (95% over 22bp)
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC21010.1541764876820251No Hit
ATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT20300.14896633507592147TruSeq Adapter, Index 20 (95% over 21bp)
ATGGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAA19750.1449303013669679No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT18670.13700499881120462TruSeq Adapter, Index 20 (95% over 24bp)
ATTGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC18610.13656470422477332No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC16830.12350263149397823TruSeq Adapter, Index 23 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC16130.11836586131894647No Hit
ATATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC15000.11007364660782373No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC13850.10163466703455724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA453.8562575E-1045.00000436
AACGTAT253.8911894E-545.020
AACGTAA253.8911894E-545.030
AAGTAAC207.033749E-445.035
GATACGT502.1827873E-1145.011
ACCGGTG351.2121563E-745.034
CGAACTA253.8911894E-545.026
GTTTCGA406.8175723E-945.018
GACGTAA406.8175723E-945.012
ATTACGC351.2121563E-745.01
TCTTACG207.033749E-445.03
ATAGCCG351.2121563E-745.01
TAACGGT406.8175723E-945.011
TAACGCA207.033749E-445.012
TGACGGA207.033749E-445.039
TAGCCGT207.033749E-445.019
GACTTAT207.033749E-445.011
ATTCCCG502.1827873E-1145.031
ACGATCT207.033749E-445.014
CGCTAAC253.8911894E-545.038
Produced by FastQC (version 0.11.5)