##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545624_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1362724
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89655278691797	31.0	31.0	34.0	31.0	34.0
2	32.55192687587508	34.0	31.0	34.0	31.0	34.0
3	32.52140638896798	34.0	31.0	34.0	31.0	34.0
4	36.004355981108425	37.0	35.0	37.0	35.0	37.0
5	35.77778258840382	37.0	35.0	37.0	35.0	37.0
6	35.77334588662121	37.0	35.0	37.0	35.0	37.0
7	35.75483883750488	37.0	35.0	37.0	33.0	37.0
8	35.717264097498834	37.0	35.0	37.0	33.0	37.0
9	37.93158996245755	39.0	38.0	39.0	35.0	39.0
10	37.85576389643097	39.0	38.0	39.0	35.0	39.0
11	37.69383235343327	39.0	38.0	39.0	35.0	39.0
12	37.15589877333928	39.0	37.0	39.0	33.0	39.0
13	36.92726920491604	39.0	37.0	39.0	33.0	39.0
14	37.532313953522504	40.0	35.0	41.0	33.0	41.0
15	37.39318526715608	40.0	35.0	41.0	32.0	41.0
16	37.32010810699745	40.0	35.0	41.0	32.0	41.0
17	37.46786143048776	40.0	35.0	41.0	32.0	41.0
18	37.53910036074803	40.0	35.0	41.0	32.0	41.0
19	37.53501589463457	39.0	35.0	41.0	32.0	41.0
20	37.49822120987082	39.0	35.0	41.0	32.0	41.0
21	37.48511510768138	39.0	35.0	41.0	32.0	41.0
22	37.406890169983065	39.0	35.0	41.0	32.0	41.0
23	37.33154549270432	39.0	35.0	41.0	32.0	41.0
24	37.31438647884678	39.0	35.0	41.0	32.0	41.0
25	37.257807156841736	39.0	35.0	41.0	32.0	41.0
26	37.17360668778124	39.0	35.0	41.0	32.0	41.0
27	37.082688057156105	39.0	35.0	41.0	32.0	41.0
28	36.99474435028663	39.0	35.0	41.0	31.0	41.0
29	36.91789826846816	39.0	35.0	41.0	31.0	41.0
30	36.83629773894054	39.0	35.0	41.0	31.0	41.0
31	36.72770568361605	39.0	35.0	41.0	31.0	41.0
32	36.62026353098646	39.0	35.0	41.0	31.0	41.0
33	36.47792216178772	38.0	35.0	41.0	30.0	41.0
34	36.41764509908096	38.0	35.0	41.0	30.0	41.0
35	36.332134019801515	38.0	35.0	41.0	30.0	41.0
36	36.236480020899315	38.0	35.0	41.0	30.0	41.0
37	36.08320246799792	38.0	35.0	41.0	30.0	41.0
38	36.0113918885996	38.0	35.0	41.0	30.0	41.0
39	35.935535735776284	38.0	35.0	40.0	29.0	41.0
40	35.810090671331835	38.0	35.0	40.0	29.0	41.0
41	35.73043771152486	38.0	35.0	40.0	29.0	41.0
42	35.59210302306263	38.0	34.0	40.0	28.0	41.0
43	35.46157402379352	37.0	34.0	40.0	28.0	41.0
44	35.378055277517674	37.0	34.0	40.0	28.0	41.0
45	35.34615153178487	37.0	34.0	40.0	28.0	41.0
46	35.258109492457756	37.0	34.0	40.0	28.0	41.0
47	35.15097407839005	36.0	34.0	40.0	28.0	41.0
48	35.09919029825555	36.0	34.0	40.0	28.0	41.0
49	35.02120825640409	36.0	34.0	40.0	28.0	41.0
50	34.906125525051294	36.0	34.0	40.0	27.0	41.0
51	34.705382014259676	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	11.0
11	16.0
12	9.0
13	17.0
14	23.0
15	63.0
16	97.0
17	189.0
18	398.0
19	738.0
20	1385.0
21	2229.0
22	3539.0
23	5137.0
24	7393.0
25	10651.0
26	14318.0
27	17257.0
28	20213.0
29	23645.0
30	28655.0
31	35166.0
32	43219.0
33	56685.0
34	93290.0
35	163787.0
36	100711.0
37	141786.0
38	209307.0
39	382610.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.099031058380127	91.5182384694186	2.9161444283655387	3.4665860438357288
2	3.2522359626747606	2.651453999489258	91.54722452969199	2.5490855081439823
3	27.289091554856306	16.081686386971977	43.56612197334163	13.06310008483009
4	27.61556999069511	15.807236094763136	43.21865616221627	13.35853775232549
5	23.99378010514235	13.741447277658573	46.51638923215559	15.748383385043486
6	22.962977095875615	14.58960141598739	47.4248637288255	15.022557759311498
7	22.838373727915556	16.37374846263807	45.027386323276026	15.760491486170347
8	25.416738826057223	19.43313539645592	43.417375785558924	11.732749991927932
9	84.2156592237313	3.49975490268022	8.397445117279801	3.887140756308688
10	83.99998825881103	3.876280156510049	8.674317029714015	3.4494145549649087
11	78.2884135011932	5.844396957857938	9.695653705372473	6.17153583557639
12	43.26613459511977	21.485348463812187	17.27194941895791	17.976567522110127
13	36.2309609282584	23.469022340547316	22.242288240318654	18.05772849087563
14	28.860869845984954	24.796657283499812	25.92072936265891	20.421743507856323
15	22.730648319102034	26.879617589475195	31.481356459561876	18.90837763186089
16	18.764034389942495	32.0792031255045	30.415550030673856	18.741212453879143
17	18.64442102729533	22.303782717556896	38.17317373143791	20.878622523709865
18	22.979121230711428	21.412186180033522	34.29865475327359	21.31003783598146
19	23.40085006208154	20.762531517754145	28.104737276220277	27.731881143944044
20	24.87092030374456	21.511692756566994	31.45868128836067	22.158705651327782
21	27.068503967054227	24.740886635885182	27.204408229399352	20.986201167661243
22	27.782001344366137	23.773926341650984	25.54141557644835	22.902656737534528
23	26.172284336373323	24.46753708014242	28.231762264405706	21.128416319078553
24	29.596748864773794	20.710429991693108	24.880606784646048	24.812214358887054
25	26.70518755081733	25.236438192913607	23.082370311229568	24.976003945039494
26	26.43807550171568	22.486284823632666	28.669415083318412	22.406224591333242
27	28.054617075798184	22.656678828581576	26.659617061121693	22.62908703449855
28	24.66244081706934	26.851291971081455	24.252233027377518	24.23403418447169
29	22.487605707391957	26.119155456277284	26.594673609623076	24.798565226707684
30	22.948080462368022	24.63433534596881	28.928234917708938	23.489349273954225
31	22.446218016267416	24.654368749651432	28.364951376801173	24.534461857279975
32	24.28701629970559	24.719165436287906	28.77772755158051	22.21609071242599
33	26.399109430816516	24.55141319885758	25.808967920136432	23.24050945018947
34	25.83883456958269	25.500761709634528	25.991616791074346	22.668786929708435
35	25.06912625006971	24.588911621135313	25.59175592416366	24.75020620463131
36	23.503365318288957	25.046010784282068	26.4730055389059	24.977618358523078
37	23.468215133805526	25.162321937531	29.60841667131422	21.76104625734925
38	27.244475036764598	25.824524995523674	24.66831141155509	22.26268855615664
39	24.656863752307878	27.84415626348402	24.962281430429055	22.536698553779047
40	23.878789835652707	26.377094701494947	26.768076294245937	22.97603916860641
41	22.705624910106522	26.230329839351178	25.896586542836257	25.167458707706036
42	26.505293808577523	23.21577957091825	26.99240638603268	23.286520234471546
43	23.080609132883843	24.05696237829524	26.934874560072323	25.927553928748594
44	22.329980245449555	25.656992905386566	25.859234885420673	26.153791963743206
45	22.701075199380067	22.390814280808147	27.72483643056114	27.18327408925065
46	23.873212770891243	23.991358484916976	26.964594444656438	25.170834299535343
47	20.72906912918537	26.413492387306604	26.84732931980357	26.010109163704463
48	23.299508924771267	24.366416090125366	26.128254877730196	26.205820107373174
49	22.301067567607234	25.458199899612833	28.989802777378248	23.250929755401682
50	22.755524963235402	24.462180162674173	29.50626832726216	23.276026546828266
51	22.56340975868921	23.74560072325724	27.19897792950003	26.492011588553517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	554.0
1	806.0
2	1058.0
3	3122.5
4	5187.0
5	3595.0
6	2003.0
7	2261.5
8	2520.0
9	2694.0
10	2868.0
11	2770.0
12	2672.0
13	2812.5
14	2953.0
15	3236.0
16	3519.0
17	3276.5
18	3034.0
19	3667.5
20	4301.0
21	4046.5
22	3792.0
23	4381.5
24	4971.0
25	5026.5
26	6067.5
27	7053.0
28	10068.0
29	13083.0
30	17563.5
31	22044.0
32	26595.5
33	31147.0
34	32410.0
35	33673.0
36	38145.5
37	42618.0
38	50032.0
39	57446.0
40	69071.5
41	80697.0
42	88499.5
43	96302.0
44	107382.5
45	118463.0
46	127401.0
47	136339.0
48	131716.0
49	127093.0
50	113002.5
51	98912.0
52	91308.5
53	83705.0
54	80763.5
55	77822.0
56	75912.0
57	74002.0
58	66837.5
59	59673.0
60	57675.0
61	55677.0
62	45587.0
63	35497.0
64	29044.0
65	22591.0
66	19910.0
67	17229.0
68	14208.5
69	11188.0
70	9112.0
71	7036.0
72	5933.5
73	4831.0
74	3578.0
75	1510.5
76	696.0
77	514.5
78	333.0
79	404.0
80	475.0
81	320.5
82	166.0
83	106.0
84	46.0
85	40.0
86	34.0
87	19.5
88	5.0
89	4.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1362724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.648865546709985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74744134329156	12.111623697916324
2	11.089692371441204	3.692615944930001
3	4.550447696738422	2.2727937564100236
4	2.519051781070689	1.6775741763298486
5	1.461949913998818	1.2169903777095272
6	0.9331901700788853	0.932193460269287
7	0.6410071025237463	0.7470428745082797
8	0.44266691083249016	0.589592150434207
9	0.31671377637742	0.4745632571728596
>10	2.544273169715642	9.490267079629115
>50	0.9859755964916896	12.098477606125828
>100	1.751665931696772	51.36701544444675
>500	0.008846797634832182	1.0007560912267786
>1k	0.007077438107865745	2.3284940828911833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4770	0.3500341962128795	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3068	0.22513729852853548	No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	2944	0.21603787707562208	TruSeq Adapter, Index 20 (95% over 22bp)
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	2101	0.1541764876820251	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	2030	0.14896633507592147	TruSeq Adapter, Index 20 (95% over 21bp)
ATGGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAA	1975	0.1449303013669679	No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	1867	0.13700499881120462	TruSeq Adapter, Index 20 (95% over 24bp)
ATTGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	1861	0.13656470422477332	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1683	0.12350263149397823	TruSeq Adapter, Index 23 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	1613	0.11836586131894647	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	1500	0.11007364660782373	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	1385	0.10163466703455724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.155497371441319	0.0
2	0.0	0.0	0.0	0.710048403051535	0.0
3	0.0	0.0	0.0	1.027354035006355	0.0
4	0.0	0.0	0.0	1.354492912724807	0.0
5	0.0	0.0	0.0	2.5851162818002766	0.0
6	0.0	0.0	0.0	2.857658630801248	0.0
7	0.0	0.0	0.0	3.295384832145027	0.0
8	0.0	0.0	0.0	4.666168644567792	0.0
9	0.0	0.0	0.0	4.835461913050625	0.0
10	0.0	0.0	0.0	5.163554762373011	0.0
11	0.0	0.0	0.0	5.410706790223112	0.0
12	0.0	0.0	0.0	6.081715739944405	0.0
13	0.0	0.0	0.0	6.6868272665631485	0.0
14	0.0	0.0	0.0	6.949316222507272	0.0
15	0.0	0.0	0.0	7.088889606405993	0.0
16	7.338243107188249E-5	0.0	0.0	7.193899865269857	0.0
17	7.338243107188249E-5	0.0	0.0	7.326868830372107	0.0
18	7.338243107188249E-5	0.0	0.0	7.6375700435304585	0.0
19	1.4676486214376498E-4	0.0	0.0	8.014168679791359	0.0
20	1.4676486214376498E-4	0.0	0.0	8.43516368685075	0.0
21	2.9352972428752996E-4	0.0	0.0	8.691782048309122	0.0
22	2.9352972428752996E-4	0.0	0.0	8.98876074685703	0.0
23	3.6691215535941245E-4	0.0	0.0	9.44519946812414	0.0
24	5.136770175031774E-4	0.0	0.0	9.82722840428436	0.0
25	5.136770175031774E-4	0.0	0.0	10.208376751271718	0.0
26	7.338243107188249E-4	0.0	0.0	10.523921204880812	0.0
27	7.338243107188249E-4	0.0	0.0	10.819872549393715	0.0
28	7.338243107188249E-4	0.0	0.0	11.085443567442857	0.0
29	7.338243107188249E-4	0.0	0.0	11.367085337896743	0.0
30	7.338243107188249E-4	0.0	0.0	11.625611642562983	0.0
31	7.338243107188249E-4	0.0	0.0	11.911289446725823	0.0
32	8.805891728625899E-4	0.0	0.0	12.220963305849168	0.0
33	8.805891728625899E-4	0.0	0.0	12.49159771164227	0.0
34	8.805891728625899E-4	0.0	0.0	12.782118756255851	0.0
35	8.805891728625899E-4	0.0	0.0	13.066475676659397	0.0
36	8.805891728625899E-4	0.0	0.0	13.355308925358326	0.0
37	9.539716039344724E-4	0.0	0.0	13.710773421470526	0.0
38	9.539716039344724E-4	0.0	0.0	14.001074318790891	0.0
39	0.0012475013282220023	0.0	0.0	14.328800255957919	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACA	45	3.8562575E-10	45.000004	36
AACGTAT	25	3.8911894E-5	45.0	20
AACGTAA	25	3.8911894E-5	45.0	30
AAGTAAC	20	7.033749E-4	45.0	35
GATACGT	50	2.1827873E-11	45.0	11
ACCGGTG	35	1.2121563E-7	45.0	34
CGAACTA	25	3.8911894E-5	45.0	26
GTTTCGA	40	6.8175723E-9	45.0	18
GACGTAA	40	6.8175723E-9	45.0	12
ATTACGC	35	1.2121563E-7	45.0	1
TCTTACG	20	7.033749E-4	45.0	3
ATAGCCG	35	1.2121563E-7	45.0	1
TAACGGT	40	6.8175723E-9	45.0	11
TAACGCA	20	7.033749E-4	45.0	12
TGACGGA	20	7.033749E-4	45.0	39
TAGCCGT	20	7.033749E-4	45.0	19
GACTTAT	20	7.033749E-4	45.0	11
ATTCCCG	50	2.1827873E-11	45.0	31
ACGATCT	20	7.033749E-4	45.0	14
CGCTAAC	25	3.8911894E-5	45.0	38
>>END_MODULE