FastQCFastQC Report
Thu 26 May 2016
SRR1545623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545623_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1137585
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT54100.4755688585907867TruSeq Adapter, Index 20 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC34250.3010764030819675TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT31630.27804515706518634TruSeq Adapter, Index 23 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC30530.26837554995890417TruSeq Adapter, Index 20 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC28010.24622335913360321TruSeq Adapter, Index 23 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC24760.21765406541049678TruSeq Adapter, Index 23 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC23490.2064900644786983TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC22750.19998505606174483TruSeq Adapter, Index 23 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC16070.14126416927086768TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC14380.12640813653485233TruSeq Adapter, Index 20 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC14270.12544117582422412TruSeq Adapter, Index 20 (95% over 23bp)
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAGT12990.11418926937327759No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT12450.10944237133928454TruSeq Adapter, Index 23 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC11460.10073972494363059TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC351.01956175E-746.00000428
AGATCCG351.01956175E-746.00000416
GTATTCG351.01956175E-746.0000041
TAAGTCG351.01956175E-746.00000423
AGTATAC351.01956175E-746.00000436
GTGTCGA551.8189894E-1246.00000431
TGTTACG1100.046.0000041
CGTAAGT351.01956175E-746.00000421
CGTAAAG351.01956175E-746.00000430
ACGTATA551.8189894E-1246.00000419
CACGTAA351.01956175E-746.00000419
CCACGTA351.01956175E-746.00000418
TGTCGAT551.8189894E-1246.00000432
TACGTAG351.01956175E-746.0000041
CGGAATA206.311429E-446.019
AGCGTAT206.311429E-446.013
ACCGGTG453.092282E-1046.032
CGAACGA206.311429E-446.026
CAAGTCG206.311429E-446.034
GTCGAAA253.417133E-546.012
Produced by FastQC (version 0.11.5)