##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545623_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1137585
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.543613004742504	33.0	31.0	34.0	31.0	34.0
2	32.96237643780465	34.0	31.0	34.0	31.0	34.0
3	33.04487488846987	34.0	33.0	34.0	31.0	34.0
4	36.42121863421195	37.0	37.0	37.0	35.0	37.0
5	36.391765890021404	37.0	37.0	37.0	35.0	37.0
6	36.39935916876541	37.0	37.0	37.0	35.0	37.0
7	36.48731127783858	37.0	37.0	37.0	35.0	37.0
8	36.522704677013145	37.0	37.0	37.0	35.0	37.0
9	38.34139075321844	39.0	39.0	39.0	37.0	39.0
10	38.06430728253273	39.0	39.0	39.0	35.0	39.0
11	37.894305040941994	39.0	38.0	39.0	35.0	39.0
12	37.45217456277992	39.0	37.0	39.0	35.0	39.0
13	37.33134051521425	39.0	37.0	39.0	35.0	39.0
14	38.514467050813785	40.0	38.0	41.0	35.0	41.0
15	38.617182891827866	40.0	38.0	41.0	35.0	41.0
16	38.70313251317484	41.0	37.0	41.0	35.0	41.0
17	38.681927943845956	40.0	37.0	41.0	35.0	41.0
18	38.67043078099658	40.0	37.0	41.0	35.0	41.0
19	38.64112923429897	40.0	37.0	41.0	35.0	41.0
20	38.53621663436139	40.0	37.0	41.0	35.0	41.0
21	38.500541058470354	40.0	37.0	41.0	35.0	41.0
22	38.625276352975824	40.0	37.0	41.0	35.0	41.0
23	38.6412092283214	40.0	37.0	41.0	35.0	41.0
24	38.60813565579715	40.0	37.0	41.0	35.0	41.0
25	38.528854547132745	40.0	36.0	41.0	35.0	41.0
26	38.42456080204995	40.0	36.0	41.0	35.0	41.0
27	38.32157684920248	40.0	36.0	41.0	35.0	41.0
28	38.260790182711624	40.0	36.0	41.0	34.0	41.0
29	38.18544899941543	40.0	36.0	41.0	34.0	41.0
30	38.13271623658891	40.0	36.0	41.0	34.0	41.0
31	38.04630862748718	40.0	35.0	41.0	34.0	41.0
32	37.99471863640959	40.0	35.0	41.0	34.0	41.0
33	37.933370253651375	40.0	35.0	41.0	34.0	41.0
34	37.828518308522	40.0	35.0	41.0	34.0	41.0
35	37.72999907699205	40.0	35.0	41.0	33.0	41.0
36	37.67031210854574	40.0	35.0	41.0	33.0	41.0
37	37.56314033676605	40.0	35.0	41.0	33.0	41.0
38	37.43726227051165	40.0	35.0	41.0	33.0	41.0
39	37.32325232839744	39.0	35.0	41.0	33.0	41.0
40	37.17777484759381	39.0	35.0	41.0	33.0	41.0
41	37.03063946869904	39.0	35.0	41.0	33.0	41.0
42	36.971729585042	39.0	35.0	41.0	33.0	41.0
43	36.89406857509549	39.0	35.0	41.0	32.0	41.0
44	36.731666644690286	38.0	35.0	41.0	32.0	41.0
45	36.62036419256583	38.0	35.0	41.0	32.0	41.0
46	36.62844886316187	38.0	35.0	41.0	32.0	41.0
47	36.568966714575176	38.0	35.0	40.0	32.0	41.0
48	36.46123058936255	38.0	35.0	40.0	32.0	41.0
49	36.37314222673471	37.0	35.0	40.0	32.0	41.0
50	36.203484574779026	37.0	35.0	40.0	32.0	41.0
51	35.97176474724966	37.0	35.0	40.0	31.0	41.0
52	35.599619369102086	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	11.0
14	19.0
15	27.0
16	49.0
17	108.0
18	201.0
19	323.0
20	604.0
21	879.0
22	1430.0
23	2085.0
24	2810.0
25	3724.0
26	4667.0
27	6218.0
28	7740.0
29	9753.0
30	12238.0
31	15487.0
32	20374.0
33	29657.0
34	70948.0
35	130309.0
36	68471.0
37	103342.0
38	176464.0
39	469045.0
40	593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.41455363775015	13.71167868774641	47.841875552156544	12.031892122346902
2	25.535146824193355	12.90373906125696	49.66670622415028	11.894407890399398
3	25.079356707410877	13.486288936650887	45.55729901501866	15.87705534091958
4	23.43121612890465	14.26117608794068	47.37782231657415	14.929785466580519
5	23.09445008504859	16.16573706580168	44.71780130715507	16.02201154199466
6	24.50208116316583	19.209201949744415	44.06765208753632	12.22106479955344
7	87.00202622221637	2.5582264182456695	6.166220546156991	4.273526813380978
8	88.01469780280155	2.445179920621316	6.57023431216129	2.969887964415846
9	81.23744599304668	3.82687887058989	8.473916234830805	6.461758901532633
10	44.48203870480008	20.448318147654902	15.663796551466483	19.405846596078536
11	36.88401306276015	21.789668464334532	22.89560780073577	18.430710672169553
12	29.345323646145122	24.6477406083941	24.97167244645455	21.03526329900623
13	23.53037355450362	25.398102119841592	32.44882799966596	18.622696325988827
14	18.51474834847506	32.723796463560966	29.863262964965255	18.898192222998723
15	18.467806801250017	22.041341965655313	37.44449865284792	22.04635258024675
16	22.89912402150169	22.28835647446125	33.02513658320038	21.787382920836684
17	23.647199989451337	21.578607312860136	26.37833656386116	28.395856133827362
18	24.919456568080626	21.718904521420377	29.7679733822088	23.593665528290195
19	26.75589076860191	25.347380635293188	26.690225345798336	21.20650325030657
20	28.00432495154208	23.059903216023418	24.254539221245004	24.681232611189493
21	26.057833041047484	25.21068755301802	27.927935055402454	20.80354435053205
22	29.71109851132003	20.301076403081968	24.655036766483384	25.332788319114613
23	26.70068610257695	25.117156080644527	21.995103662583453	26.18705415419507
24	26.1125981794767	21.606209645872614	27.598289358597377	24.682902816053307
25	29.669519200763023	22.450278440731903	24.31115037557633	23.569051982928748
26	24.652311695389795	26.15066126926779	23.385505258947685	25.81152177639473
27	23.939837462695095	24.501202107974347	25.257365383685617	26.301595045644945
28	24.9605963510419	23.670055424429822	27.386437057450653	23.982911167077624
29	22.74054246495866	24.96929899743755	25.98021246763978	26.309946069964003
30	25.643534329302863	23.9640114804608	26.409191401082115	23.983262789154217
31	26.84819156370733	23.574590030635072	23.908279381320956	25.668939024336645
32	26.12007014860428	25.57760518994185	24.765006570937555	23.53731809051631
33	25.259299305106868	23.45969751710861	25.28602258292787	25.99498059485665
34	22.80365862770694	25.220357160124294	25.628414580009405	26.347569632159356
35	23.64711208393219	23.875402717159595	29.91328120536048	22.564203993547736
36	27.359537968591358	24.14685496028868	24.913742709336006	23.579864361783955
37	25.41963897203286	27.815679707450432	23.655023580655513	23.1096577398612
38	22.953010104739427	24.928598742072023	27.902178738292083	24.216212414896468
39	22.65624107209571	25.635974454656136	25.65179744810278	26.05598702514537
40	27.22961361129059	21.963281864651872	27.843897379096948	22.963207144960595
41	23.206353810924018	23.05594746766176	27.149971210942482	26.587727510471744
42	22.84233705613207	25.52872972129555	24.885349226651197	26.743583995921185
43	23.150182184188434	21.141453166137037	27.989381013286916	27.718983636387613
44	26.33543867051693	22.18489167842403	25.16128465125683	26.318384999802213
45	21.29669431295244	24.000404365388082	26.622801812611808	28.080099509047674
46	23.758400471173584	23.05629908973835	25.573385725022746	27.61191471406532
47	22.970942830645622	23.85351424289174	29.667497373822616	23.508045552640024
48	22.301981829929193	22.705292351780308	30.845343424886934	24.147382393403568
49	22.90176118707613	21.921350932018267	26.538412514229705	28.638475366675898
50	21.89673738665682	23.50857298575491	28.43321597946527	26.161473648122996
51	24.147997732037606	23.625135704145183	26.89434196126004	25.33252460255717
52	22.919606007463177	21.85243300500622	27.81365788051003	27.414303107020576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	162.5
2	117.0
3	101.5
4	86.0
5	111.0
6	136.0
7	247.0
8	358.0
9	460.5
10	563.0
11	533.5
12	504.0
13	640.5
14	852.5
15	928.0
16	900.5
17	873.0
18	922.0
19	971.0
20	1123.0
21	1275.0
22	1653.5
23	2032.0
24	2022.0
25	2012.0
26	2438.0
27	2864.0
28	4177.0
29	5490.0
30	6816.0
31	8142.0
32	11719.0
33	15296.0
34	19176.5
35	23057.0
36	22066.0
37	21075.0
38	24344.5
39	36925.5
40	46237.0
41	52064.0
42	57891.0
43	63702.0
44	69513.0
45	76932.0
46	84351.0
47	98241.0
48	112131.0
49	118331.5
50	124532.0
51	111816.0
52	99100.0
53	88377.0
54	77654.0
55	71890.5
56	66127.0
57	63933.5
58	61740.0
59	58770.5
60	55801.0
61	51623.0
62	47445.0
63	44532.0
64	33984.0
65	26349.0
66	21748.5
67	17148.0
68	15186.0
69	13224.0
70	10879.5
71	8535.0
72	7088.0
73	5641.0
74	4635.0
75	3629.0
76	2809.5
77	1990.0
78	1718.0
79	1446.0
80	939.0
81	432.0
82	381.0
83	330.0
84	197.0
85	64.0
86	124.0
87	184.0
88	111.5
89	34.5
90	30.0
91	22.5
92	15.0
93	11.0
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1137585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.110452153033243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1149752553336	11.825884771644622
2	12.266021287727792	4.197543407035071
3	5.188012728525466	2.663077306822873
4	2.8253837076264383	1.9337437097320733
5	1.6991568688394534	1.4536671152387621
6	1.0887882115290575	1.1177795158892745
7	0.7436071268361119	0.8906417915068668
8	0.5553449095072653	0.7601762002043712
9	0.43811086258550275	0.6746647456794029
>10	3.174153006862796	11.952817472977326
>50	1.2392941727809774	15.640517793531103
>100	1.6542800902129766	43.33081863788936
>500	0.005663579518313957	0.641432313390069
>1k	0.006693321248916495	2.440631892618936
>5k	5.148708653012689E-4	0.47660332583989473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	5410	0.4755688585907867	TruSeq Adapter, Index 20 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3425	0.3010764030819675	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	3163	0.27804515706518634	TruSeq Adapter, Index 23 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3053	0.26837554995890417	TruSeq Adapter, Index 20 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2801	0.24622335913360321	TruSeq Adapter, Index 23 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2476	0.21765406541049678	TruSeq Adapter, Index 23 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2349	0.2064900644786983	TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2275	0.19998505606174483	TruSeq Adapter, Index 23 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1607	0.14126416927086768	TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1438	0.12640813653485233	TruSeq Adapter, Index 20 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1427	0.12544117582422412	TruSeq Adapter, Index 20 (95% over 23bp)
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAGT	1299	0.11418926937327759	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	1245	0.10944237133928454	TruSeq Adapter, Index 23 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1146	0.10073972494363059	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5018526088160445	0.0
2	0.0	0.0	0.0	1.0156603682362197	0.0
3	0.0	0.0	0.0	3.2947867631869268	0.0
4	0.0	0.0	0.0	3.7746629922159665	0.0
5	0.0	0.0	0.0	4.539441008803737	0.0
6	0.0	0.0	0.0	7.023035641292738	0.0
7	0.0	0.0	0.0	7.332199352136324	0.0
8	0.0	0.0	0.0	7.936198174202367	0.0
9	0.0	0.0	0.0	8.33291578211738	0.0
10	8.79055191480197E-5	0.0	0.0	9.569482719972573	0.0
11	8.79055191480197E-5	0.0	0.0	10.657665141505909	0.0
12	8.79055191480197E-5	0.0	0.0	11.11222458102032	0.0
13	8.79055191480197E-5	0.0	0.0	11.329175402277633	0.0
14	8.79055191480197E-5	0.0	0.0	11.474922753025048	0.0
15	8.79055191480197E-5	0.0	0.0	11.67903936848675	0.0
16	8.79055191480197E-5	0.0	0.0	12.16603594456678	0.0
17	8.79055191480197E-5	0.0	0.0	12.804054202543107	0.0
18	8.79055191480197E-5	0.0	0.0	13.524967365076016	0.0
19	8.79055191480197E-5	0.0	0.0	13.956407653054496	0.0
20	8.79055191480197E-5	0.0	0.0	14.437954086947348	0.0
21	8.79055191480197E-5	0.0	0.0	15.173107943582238	0.0
22	8.79055191480197E-5	0.0	0.0	15.771920340018548	0.0
23	8.79055191480197E-5	0.0	0.0	16.389456612033385	0.0
24	4.395275957400985E-4	0.0	0.0	16.884013062760147	0.0
25	4.395275957400985E-4	0.0	0.0	17.316859839044994	0.0
26	4.395275957400985E-4	0.0	0.0	17.702940879143096	0.0
27	4.395275957400985E-4	0.0	0.0	18.12945845804929	0.0
28	4.395275957400985E-4	0.0	0.0	18.505957796560256	0.0
29	4.395275957400985E-4	0.0	0.0	18.922805768360167	0.0
30	4.395275957400985E-4	0.0	0.0	19.356883221913087	0.0
31	4.395275957400985E-4	0.0	0.0	19.746480482777113	0.0
32	4.395275957400985E-4	0.0	0.0	20.1554169578537	0.0
33	4.395275957400985E-4	0.0	0.0	20.55081598298149	0.0
34	4.395275957400985E-4	0.0	0.0	20.938567227943405	0.0
35	4.395275957400985E-4	0.0	0.0	21.416685346589485	0.0
36	4.395275957400985E-4	0.0	0.0	21.797492055538708	0.0
37	0.0012306772680722759	0.0	0.0	22.22304267373427	0.0
38	0.0012306772680722759	0.0	0.0	22.657295938325486	0.0
39	0.0012306772680722759	0.0	0.0	23.33416843576524	0.0
40	0.0012306772680722759	0.0	0.0	23.76736683412668	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	35	1.01956175E-7	46.000004	28
AGATCCG	35	1.01956175E-7	46.000004	16
GTATTCG	35	1.01956175E-7	46.000004	1
TAAGTCG	35	1.01956175E-7	46.000004	23
AGTATAC	35	1.01956175E-7	46.000004	36
GTGTCGA	55	1.8189894E-12	46.000004	31
TGTTACG	110	0.0	46.000004	1
CGTAAGT	35	1.01956175E-7	46.000004	21
CGTAAAG	35	1.01956175E-7	46.000004	30
ACGTATA	55	1.8189894E-12	46.000004	19
CACGTAA	35	1.01956175E-7	46.000004	19
CCACGTA	35	1.01956175E-7	46.000004	18
TGTCGAT	55	1.8189894E-12	46.000004	32
TACGTAG	35	1.01956175E-7	46.000004	1
CGGAATA	20	6.311429E-4	46.0	19
AGCGTAT	20	6.311429E-4	46.0	13
ACCGGTG	45	3.092282E-10	46.0	32
CGAACGA	20	6.311429E-4	46.0	26
CAAGTCG	20	6.311429E-4	46.0	34
GTCGAAA	25	3.417133E-5	46.0	12
>>END_MODULE