SRR1545622_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545622_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2857786
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	7079	0.24770924064992972	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5708	0.19973503964257644	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	4128	0.1444474848711555	No Hit
ATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	3803	0.13307504480741383	RNA PCR Primer, Index 38 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	3360	0.11757353419745216	No Hit
ATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	2989	0.10459145646315016	RNA PCR Primer, Index 38 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	40	6.8212103E-9	45.000004	26
CGATTGA	30	2.1666383E-6	45.000004	32
ACGGATA	20	7.0350483E-4	45.000004	1
CGCCGAT	25	3.8922662E-5	45.000004	41
GTACGAC	40	6.8212103E-9	45.000004	39
TAACGCG	55	1.8189894E-12	45.0	3
AGTACGG	120	0.0	43.125004	4
ATAGCGC	155	0.0	40.645164	1
ATGCGAT	385	0.0	40.324677	1
CGTTTTT	3095	0.0	39.474957	1
CGTAAGA	75	0.0	39.000004	17
TAACGGG	300	0.0	38.250004	5
TCGCGAT	100	0.0	38.250004	2
ATCGGAT	165	0.0	38.18182	1
ACGGGTA	160	0.0	37.968754	7
CGTTACG	60	1.5643309E-10	37.500004	3
TACGACA	30	1.1403464E-4	37.500004	42
TAATGCG	60	1.5643309E-10	37.500004	10
ATCGTGT	715	0.0	37.44755	1
ATCGATT	175	0.0	37.285717	1