##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545622_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2857786
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861619449461926	31.0	31.0	34.0	31.0	34.0
2	32.51694003679772	33.0	31.0	34.0	31.0	34.0
3	32.49979564600009	34.0	31.0	34.0	31.0	34.0
4	35.99006118722676	37.0	35.0	37.0	35.0	37.0
5	35.76762150839846	37.0	35.0	37.0	35.0	37.0
6	35.778359891188494	37.0	35.0	37.0	35.0	37.0
7	35.761898896558385	37.0	35.0	37.0	35.0	37.0
8	35.730749258341945	37.0	35.0	37.0	33.0	37.0
9	37.904182818447566	39.0	38.0	39.0	35.0	39.0
10	37.82684182790454	39.0	38.0	39.0	35.0	39.0
11	37.660962367371106	39.0	38.0	39.0	35.0	39.0
12	37.11900646164548	39.0	37.0	39.0	33.0	39.0
13	36.933411039175084	39.0	37.0	39.0	33.0	39.0
14	37.74299405203889	40.0	36.0	41.0	33.0	41.0
15	37.72456300086851	40.0	36.0	41.0	33.0	41.0
16	37.646192891980014	40.0	36.0	41.0	32.0	41.0
17	37.72180947068815	40.0	36.0	41.0	32.0	41.0
18	37.796664270872625	40.0	36.0	41.0	33.0	41.0
19	37.74101979644382	40.0	36.0	41.0	33.0	41.0
20	37.709420859364556	40.0	36.0	41.0	33.0	41.0
21	37.69532918140127	40.0	36.0	41.0	33.0	41.0
22	37.634867341361456	40.0	36.0	41.0	32.0	41.0
23	37.58936813323321	40.0	35.0	41.0	32.0	41.0
24	37.57899786758001	40.0	35.0	41.0	32.0	41.0
25	37.5232774602437	40.0	35.0	41.0	32.0	41.0
26	37.389237682597646	40.0	35.0	41.0	32.0	41.0
27	37.30119225162416	40.0	35.0	41.0	32.0	41.0
28	37.22977647731496	39.0	35.0	41.0	32.0	41.0
29	37.176869786611036	39.0	35.0	41.0	31.0	41.0
30	37.090732476119626	39.0	35.0	41.0	31.0	41.0
31	36.972987130596906	39.0	35.0	41.0	31.0	41.0
32	36.86193962738987	39.0	35.0	41.0	31.0	41.0
33	36.773385061022765	39.0	35.0	41.0	31.0	41.0
34	36.70289902742893	39.0	35.0	41.0	30.0	41.0
35	36.62836475509363	39.0	35.0	41.0	30.0	41.0
36	36.50709115378128	39.0	35.0	41.0	30.0	41.0
37	36.382116785511585	39.0	35.0	41.0	30.0	41.0
38	36.29978591818981	38.0	35.0	41.0	30.0	41.0
39	36.25359141657213	38.0	35.0	41.0	30.0	41.0
40	36.140309316372885	38.0	35.0	41.0	30.0	41.0
41	36.07039610383703	38.0	35.0	40.0	30.0	41.0
42	35.95436012353619	38.0	35.0	40.0	29.0	41.0
43	35.8404478151968	38.0	35.0	40.0	29.0	41.0
44	35.740631733796725	38.0	35.0	40.0	29.0	41.0
45	35.71833020387111	38.0	35.0	40.0	29.0	41.0
46	35.64806462065389	38.0	34.0	40.0	28.0	41.0
47	35.55867094317069	37.0	34.0	40.0	28.0	41.0
48	35.47313514727835	37.0	34.0	40.0	28.0	41.0
49	35.4147833322719	37.0	34.0	40.0	28.0	41.0
50	35.32347208643334	37.0	34.0	40.0	28.0	41.0
51	35.119478155467206	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	11.0
10	17.0
11	42.0
12	34.0
13	40.0
14	59.0
15	117.0
16	201.0
17	459.0
18	970.0
19	1678.0
20	2841.0
21	4598.0
22	7146.0
23	10181.0
24	14445.0
25	20187.0
26	26563.0
27	33431.0
28	39282.0
29	46986.0
30	57320.0
31	70144.0
32	87346.0
33	112056.0
34	179664.0
35	271491.0
36	220115.0
37	307047.0
38	462070.0
39	880794.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.4076330418022907	91.04047678867488	3.3816737852309444	3.170216384291896
2	3.3965454376219917	2.76280309302376	90.7832146983714	3.057436770982852
3	29.776477314956402	15.914837570062979	41.1356203718543	13.173064743126323
4	27.576767469642583	15.869592754670926	42.23290337345064	14.320736402235855
5	24.979512111823627	14.824308048258336	45.30584165504345	14.890338184874585
6	23.583186424735793	14.936422811225194	46.331985670025674	15.148405094013336
7	22.930793278433026	17.60982102928631	44.00651413366851	15.452871558612156
8	25.90480882753292	19.31047321247987	43.136295019991	11.648422939996207
9	83.25007540802567	3.922477050415951	8.635356181323584	4.192091360234811
10	83.48070849251833	4.287164959167692	8.492238397136804	3.7398881511771704
11	77.41748332450365	6.918747589917509	9.538257938138125	6.125511147440712
12	40.65766296006769	23.416798878572433	18.792099898312888	17.133438263046987
13	32.98637476703994	24.882933851590007	23.50676362750745	18.623927753862606
14	26.888192467875484	24.174168394694355	28.06413776258964	20.873501374840522
15	23.40493654878287	25.231875304868872	31.400321787565623	19.962866358782637
16	20.611725300634827	28.003951310559994	31.08329315071178	20.301030238093404
17	20.04271838409174	23.295761124170948	35.40020141466156	21.26131907707575
18	24.751363468083333	22.001962358273154	31.36721923894931	21.879454934694202
19	24.34003105900862	21.796348641920705	29.194593297048833	24.669027002021846
20	24.883353757069283	22.54357044229344	30.84020287033389	21.732872930303387
21	26.09723051341143	25.332197722292708	26.94589447915274	21.624677285143115
22	26.202311859600403	23.739356270903418	27.48680272070757	22.571529148788606
23	26.050481036718637	24.353468034345468	28.349358559388282	21.246692369547617
24	28.808350240360898	20.450796525702064	26.935151897307918	23.805701336629127
25	27.522809615555538	23.520970429556307	25.622457384842672	23.333762570045483
26	26.165185216807696	22.66408331484583	28.68951698972561	22.481214478620863
27	28.021272411580156	23.711957438380622	24.938781280333796	23.32798886970543
28	24.155447608743273	25.162905829897692	25.26322824732153	25.41841831403751
29	22.833795112720125	23.56792985898874	26.656194690575152	26.94208033771598
30	22.49657602073773	24.41197486445801	28.2157236406085	24.875725474195757
31	22.778052660346155	24.836709256746307	27.154832447216133	25.230405635691405
32	23.887477928718244	24.417433635688607	28.132617347835005	23.562471087758148
33	25.822157432361976	25.17242368742796	26.390639467055966	22.6147794131541
34	25.881049175830523	24.87089656118408	24.95186833443792	24.296185928547484
35	25.353472933242728	24.829605855721876	25.332687612018535	24.48423359901686
36	23.33498729436004	24.45459527060459	28.194798350891215	24.01561908414416
37	23.324874570734128	26.58442584574212	27.475045367287827	22.615654216235924
38	24.71815594309721	27.776047611682607	24.987490315929882	22.5183061292903
39	24.83891376051251	26.95954140722923	24.974648206688673	23.226896625569584
40	23.801082376357083	25.01565897516469	28.54496452848464	22.638294119993592
41	23.46081896964993	26.71501644979715	24.89304657521592	24.931118005336998
42	25.564580412949045	24.709617865018586	27.050415951369345	22.675385770663024
43	23.177942645110587	23.97754765402308	27.94306501606488	24.90144468480145
44	22.43408008857206	25.488297584213793	26.36261077631425	25.71501155089989
45	22.426906703301086	23.416413965216428	27.487642531666122	26.66903679981636
46	23.614574359311717	24.0580295375511	26.688422436109633	25.638973667027553
47	21.989855083620675	24.785970678000382	26.719320480959734	26.504853757419205
48	21.877355407297816	25.680859238585395	26.78139650764613	25.66038884647066
49	22.266187881107964	25.142645390522596	29.285082927832946	23.3060838005365
50	22.147389622595952	23.949623939651186	29.735326577987298	24.167659859765568
51	22.91973576747874	24.536791768172982	27.74007570895791	24.80339675539036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	604.0
1	1684.0
2	2764.0
3	6313.0
4	9862.0
5	7055.0
6	4248.0
7	4223.5
8	4199.0
9	4786.0
10	5373.0
11	5291.0
12	5209.0
13	5243.5
14	5278.0
15	5076.0
16	4874.0
17	5621.5
18	6369.0
19	7201.5
20	8034.0
21	8376.0
22	8718.0
23	9827.5
24	10937.0
25	13929.5
26	24709.5
27	32497.0
28	37339.5
29	42182.0
30	47456.5
31	52731.0
32	63899.0
33	75067.0
34	84732.0
35	94397.0
36	99809.5
37	105222.0
38	118675.5
39	132129.0
40	141229.0
41	150329.0
42	157836.0
43	165343.0
44	185437.0
45	205531.0
46	229537.0
47	253543.0
48	248614.0
49	243685.0
50	222503.5
51	201322.0
52	192807.0
53	184292.0
54	177810.0
55	171328.0
56	164980.5
57	158633.0
58	144538.0
59	130443.0
60	122189.5
61	113936.0
62	97278.5
63	80621.0
64	70676.0
65	60731.0
66	50744.5
67	40758.0
68	33620.5
69	26483.0
70	22425.0
71	18367.0
72	14947.5
73	11528.0
74	9880.0
75	5638.0
76	3044.0
77	2089.0
78	1134.0
79	776.5
80	419.0
81	309.0
82	199.0
83	143.0
84	87.0
85	93.5
86	100.0
87	60.5
88	21.0
89	21.5
90	22.0
91	29.0
92	36.0
93	19.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2857786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.396326948953156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79739901775852	12.100062822775232
2	10.052739307399657	3.296560008334353
3	3.8632880858392893	1.9003120366024913
4	1.998916908681148	1.3109958071410734
5	1.2840950944072886	1.0527221500724386
6	0.8450308344012656	0.8313241105673904
7	0.651101136336848	0.7472966975749717
8	0.47390457836838185	0.6216235527627025
9	0.374919550573838	0.5532573177686904
>10	3.336655245523449	12.783998328682342
>50	1.6313560988438989	19.71995284124647
>100	1.6802869532140952	42.534324422830025
>500	0.0062272598108107785	0.6871611842582929
>1k	0.003650462647716664	1.4101996676280886
>5k	4.294661938490193E-4	0.4502090517554333
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	7079	0.24770924064992972	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5708	0.19973503964257644	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	4128	0.1444474848711555	No Hit
ATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	3803	0.13307504480741383	RNA PCR Primer, Index 38 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	3360	0.11757353419745216	No Hit
ATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	2989	0.10459145646315016	RNA PCR Primer, Index 38 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.998424654610248E-5	0.0	0.0	0.08741032393608199	0.0
2	1.0497636981915371E-4	0.0	0.0	0.4360018559822184	0.0
3	1.0497636981915371E-4	0.0	0.0	0.5918567730403886	0.0
4	1.0497636981915371E-4	0.0	0.0	0.8326025811589811	0.0
5	1.0497636981915371E-4	0.0	0.0	1.766017469467623	0.0
6	1.0497636981915371E-4	0.0	0.0	1.9796793741728738	0.0
7	1.0497636981915371E-4	0.0	0.0	2.3303004493688473	0.0
8	1.0497636981915371E-4	0.0	0.0	3.413411641039602	0.0
9	1.3996849309220495E-4	0.0	0.0	3.5463467173539236	0.0
10	1.3996849309220495E-4	0.0	0.0	3.8066181302588786	0.0
11	1.3996849309220495E-4	0.0	0.0	3.9943858637420715	0.0
12	1.749606163652562E-4	0.0	0.0	4.60401863540517	0.0
13	2.0995273963830743E-4	0.0	0.0	5.1088150057422075	0.0
14	2.0995273963830743E-4	0.0	0.0	5.310649572781167	0.0
15	2.4494486291135866E-4	0.0	0.0	5.4290629179371725	0.0
16	2.4494486291135866E-4	0.0	0.0	5.533199476797773	0.0
17	2.4494486291135866E-4	0.0	0.0	5.652102711679601	0.0
18	2.4494486291135866E-4	0.0	0.0	5.903836046505932	0.0
19	2.4494486291135866E-4	0.0	0.0	6.224608840549992	0.0
20	2.4494486291135866E-4	0.0	0.0	6.565852026708788	0.0
21	2.4494486291135866E-4	0.0	0.0	6.788541899218486	0.0
22	2.799369861844099E-4	0.0	0.0	7.045873973768504	0.0
23	2.799369861844099E-4	0.0	0.0	7.4250486215552876	0.0
24	3.1492910945746114E-4	0.0	0.0	7.780568594009488	0.0
25	3.499212327305124E-4	0.0	0.0	8.151310140087466	0.0
26	3.499212327305124E-4	0.0	0.0	8.45112265229097	0.0
27	3.849133560035636E-4	0.0	0.0	8.716118001837787	0.0
28	4.548976025496661E-4	0.0	0.0	8.95227984180761	0.0
29	4.898897258227173E-4	0.0	0.0	9.207757333824157	0.0
30	4.898897258227173E-4	0.0	0.0	9.457321157007557	0.0
31	5.248818490957686E-4	0.0	0.0	9.748245669899706	0.0
32	5.598739723688198E-4	0.0	0.0	10.056596260181832	0.0
33	5.94866095641871E-4	0.0	0.0	10.314488208704221	0.0
34	6.298582189149223E-4	0.0	0.0	10.57664919626592	0.0
35	6.648503421879735E-4	0.0	0.0	10.84752322252261	0.0
36	6.648503421879735E-4	0.0	0.0	11.14856045904067	0.0
37	6.648503421879735E-4	0.0	0.0	11.481440527737206	0.0
38	6.648503421879735E-4	0.0	0.0	11.760432726593244	0.0
39	6.648503421879735E-4	0.0	0.0	12.052546971676676	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	40	6.8212103E-9	45.000004	26
CGATTGA	30	2.1666383E-6	45.000004	32
ACGGATA	20	7.0350483E-4	45.000004	1
CGCCGAT	25	3.8922662E-5	45.000004	41
GTACGAC	40	6.8212103E-9	45.000004	39
TAACGCG	55	1.8189894E-12	45.0	3
AGTACGG	120	0.0	43.125004	4
ATAGCGC	155	0.0	40.645164	1
ATGCGAT	385	0.0	40.324677	1
CGTTTTT	3095	0.0	39.474957	1
CGTAAGA	75	0.0	39.000004	17
TAACGGG	300	0.0	38.250004	5
TCGCGAT	100	0.0	38.250004	2
ATCGGAT	165	0.0	38.18182	1
ACGGGTA	160	0.0	37.968754	7
CGTTACG	60	1.5643309E-10	37.500004	3
TACGACA	30	1.1403464E-4	37.500004	42
TAATGCG	60	1.5643309E-10	37.500004	10
ATCGTGT	715	0.0	37.44755	1
ATCGATT	175	0.0	37.285717	1
>>END_MODULE