FastQCFastQC Report
Thu 26 May 2016
SRR1545621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545621_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2355588
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT59150.25110503194955996RNA PCR Primer, Index 27 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC56080.2380721925905549RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT54530.23149209454284875RNA PCR Primer, Index 38 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC52330.22215260053965294RNA PCR Primer, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC51300.21778001925633855RNA PCR Primer, Index 27 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC40320.17116745373129766RNA PCR Primer, Index 38 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC38030.1614458895188802RNA PCR Primer, Index 27 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC37340.15851668458151424RNA PCR Primer, Index 38 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC31440.13346985975476186RNA PCR Primer, Index 38 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC23740.10078163074357656RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATT453.110472E-1046.00000431
TAACGCG206.3128106E-446.01
ATCCGAT206.3128106E-446.020
GCTCGAA253.418257E-546.019
ACGGGTA1600.043.1255
TCGTTAG1650.041.818181
CCGTCAA554.7293724E-1141.8181835
TAGTACG508.731149E-1041.3999981
TACGTTG451.5896148E-840.8888931
TACGGGT1350.039.1851844
TACTATT21900.039.17351546
ACGACCA18200.038.67032628
AACACGT17750.038.4845141
ACTACGC301.0018856E-438.33333230
TACTACG301.0018856E-438.33333229
TCGAATT301.0018856E-438.33333221
AAACACG17900.038.0335240
CGACCAA18400.038.029
CACGACC18800.037.92553327
ATCGTAG850.037.8823551
Produced by FastQC (version 0.11.5)