##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545621_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2355588
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.505478462277786	33.0	31.0	34.0	31.0	34.0
2	32.92439042820731	34.0	31.0	34.0	31.0	34.0
3	33.01820055119995	34.0	33.0	34.0	31.0	34.0
4	36.41302511305033	37.0	37.0	37.0	35.0	37.0
5	36.39078183451436	37.0	37.0	37.0	35.0	37.0
6	36.39756909951995	37.0	37.0	37.0	35.0	37.0
7	36.469928951921986	37.0	37.0	37.0	35.0	37.0
8	36.503545611541576	37.0	37.0	37.0	35.0	37.0
9	38.32399171671786	39.0	39.0	39.0	37.0	39.0
10	38.04270823250925	39.0	39.0	39.0	35.0	39.0
11	37.896672932618095	39.0	38.0	39.0	35.0	39.0
12	37.57844453274512	39.0	37.0	39.0	35.0	39.0
13	37.526887129667834	39.0	37.0	39.0	35.0	39.0
14	38.7378862517554	41.0	38.0	41.0	35.0	41.0
15	38.81179306398233	41.0	38.0	41.0	35.0	41.0
16	38.91491126631652	41.0	38.0	41.0	35.0	41.0
17	38.86053078891555	41.0	38.0	41.0	35.0	41.0
18	38.83870354238517	40.0	38.0	41.0	35.0	41.0
19	38.81078524767489	40.0	38.0	41.0	35.0	41.0
20	38.725596326692106	40.0	38.0	41.0	35.0	41.0
21	38.69763685330372	40.0	38.0	41.0	35.0	41.0
22	38.83699059428049	40.0	38.0	41.0	35.0	41.0
23	38.84921217122859	41.0	38.0	41.0	35.0	41.0
24	38.77682217773227	41.0	38.0	41.0	35.0	41.0
25	38.68948389956138	40.0	38.0	41.0	35.0	41.0
26	38.58871712710372	40.0	38.0	41.0	35.0	41.0
27	38.50341485862553	40.0	37.0	41.0	35.0	41.0
28	38.435342683015875	40.0	37.0	41.0	35.0	41.0
29	38.35337970816629	40.0	37.0	41.0	34.0	41.0
30	38.29709439851111	40.0	37.0	41.0	34.0	41.0
31	38.249045673521856	40.0	37.0	41.0	34.0	41.0
32	38.190548177355296	40.0	36.0	41.0	34.0	41.0
33	38.132498127855975	40.0	36.0	41.0	34.0	41.0
34	38.009239731226344	40.0	36.0	41.0	34.0	41.0
35	37.905272908505225	40.0	36.0	41.0	34.0	41.0
36	37.82749105531188	40.0	35.0	41.0	34.0	41.0
37	37.75498686527525	40.0	35.0	41.0	33.0	41.0
38	37.64766843777435	40.0	35.0	41.0	33.0	41.0
39	37.546542943842475	40.0	35.0	41.0	33.0	41.0
40	37.436653183833506	40.0	35.0	41.0	33.0	41.0
41	37.29715680331196	39.0	35.0	41.0	33.0	41.0
42	37.19562291877867	39.0	35.0	41.0	33.0	41.0
43	37.11808474147431	39.0	35.0	41.0	33.0	41.0
44	37.004716868994066	39.0	35.0	41.0	33.0	41.0
45	36.92408052681539	39.0	35.0	41.0	32.0	41.0
46	36.89339816640261	39.0	35.0	41.0	32.0	41.0
47	36.82286715673539	39.0	35.0	41.0	32.0	41.0
48	36.73585278919743	38.0	35.0	41.0	32.0	41.0
49	36.63582850651302	38.0	35.0	40.0	32.0	41.0
50	36.55187409682848	38.0	35.0	40.0	32.0	41.0
51	36.43711294165193	38.0	35.0	40.0	32.0	41.0
52	36.10409884920453	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	7.0
12	14.0
13	28.0
14	41.0
15	61.0
16	150.0
17	260.0
18	477.0
19	819.0
20	1389.0
21	2178.0
22	3055.0
23	4158.0
24	5722.0
25	7389.0
26	9389.0
27	11658.0
28	14836.0
29	18991.0
30	23903.0
31	30970.0
32	40424.0
33	57992.0
34	134917.0
35	201260.0
36	147634.0
37	220713.0
38	372688.0
39	1043024.0
40	1433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.064887408154565	13.607939928374572	45.41757726733198	11.909595396138883
2	26.002297515524788	12.929468141287865	48.48267184244443	12.585562500742913
3	26.079220984314745	14.442211456332771	44.687059027300194	14.791508532052294
4	23.816049326113056	14.57670016997879	46.559287956977194	15.047962546930957
5	22.782973932623193	17.0085345994291	44.60750351929115	15.600987948656556
6	25.22495444874061	18.973224519737748	43.9918186032532	11.81000242826844
7	87.78716821447554	2.4768762618929965	5.990733523859011	3.745221999772456
8	88.37186299132107	2.8306308233867723	5.959616027930181	2.8378901573619832
9	81.67400241468373	4.553809919221867	7.76039782848274	6.01178983761167
10	42.35036007994607	22.547661135988125	16.991978223696165	18.11000056036964
11	34.13801564619959	23.597377809701868	23.409102101046532	18.85550444305201
12	27.756848820761526	24.11117733661404	26.694566282388944	21.437407560235492
13	24.278269374780308	24.214803267804047	31.97638975915992	19.530537598255723
14	20.507406218744535	28.25536553930484	30.775203473612535	20.462024768338097
15	19.805076269704212	22.825341273601328	35.26193884499327	22.107643611701196
16	25.255435160987403	22.151072258816058	30.06183594075025	22.531656639446286
17	24.937085772214836	21.953626865139405	27.68476490795504	25.424522454690717
18	25.48119620239193	22.29872116855749	29.330765821527365	22.889316807523215
19	26.124984504930403	25.748008565165048	26.055193013379252	22.0718139165253
20	26.375198039725113	23.487511398427905	26.05790995708927	24.07938060475771
21	25.835417738585864	24.84139841092755	27.95480364138381	21.36838020910278
22	29.120627206455456	20.02362042937899	26.45314036240633	24.402612001759223
23	27.986897538958427	23.455247691871413	24.474059130883667	24.083795638286492
24	26.692061599906264	21.87823167718633	27.708707974399598	23.720998748507803
25	29.576521870547822	23.51892606007502	22.798256740992056	24.106295328385098
26	24.393824386947124	24.645438845842314	24.262944114165975	26.697792653044587
27	23.2720238004269	22.363460842897826	25.318519197754448	29.04599615892083
28	23.619707690818597	23.181260899614024	26.91570002903734	26.28333138053004
29	23.143011426446392	24.234670918683573	25.696598895902	26.92571875896804
30	24.406517608342376	23.991207290918446	26.12961179968653	25.472663301052645
31	25.719735369682645	24.915095509061857	25.712306226725556	23.652862894529942
32	26.41650407456652	25.62120370794893	23.15982251565214	24.802469701832408
33	25.847092106089857	23.96942928899281	24.682711917364156	25.500766687553174
34	23.15663859724196	23.84869510287877	28.17971563787895	24.814950662000314
35	23.480082255470823	26.281590838465814	26.88118635347098	23.35714055259239
36	25.031584470628985	26.611105167796744	24.889581709534944	23.46772865203932
37	26.15440391104047	26.303666006109726	23.916703600120226	23.625226482729577
38	23.387409003611836	24.005471245396055	29.622497652390827	22.984622098601285
39	23.600689084848455	26.394938333868232	24.586600033622176	25.417772547661137
40	26.59123751691722	23.602939053858314	27.412391301025473	22.393432128198988
41	23.943490967011208	22.691744057110157	27.813055593762577	25.551709382116055
42	22.906552419183658	24.736838530337224	25.59399181860325	26.76261723187586
43	22.602042462434007	22.006564815239336	27.596846307588596	27.794546414738065
44	25.67235866373916	22.59087752187564	25.157497830690257	26.579265983694945
45	21.973324707037055	23.250755225446895	26.357792619082794	28.418127448433257
46	22.286155303898646	24.487558944942833	25.984934547127935	27.241351204030583
47	22.749054588493404	24.00288165842244	29.522989589011324	23.725074164072836
48	22.016583545170036	22.751686627712488	31.005973879982406	24.225755947135067
49	23.33009847222859	22.692338388546723	27.110810549213188	26.866752590011494
50	21.613032499741042	22.975834483789185	29.924418022166865	25.48671499430291
51	22.934146378738557	22.829501593657294	28.1541169338611	26.08223509374305
52	22.673574496049394	22.45150680000068	28.107122298126836	26.76779640582309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	150.0
2	159.0
3	120.5
4	82.0
5	127.0
6	172.0
7	285.0
8	398.0
9	567.5
10	737.0
11	799.0
12	861.0
13	936.5
14	908.0
15	804.0
16	1001.5
17	1199.0
18	1954.0
19	2709.0
20	3000.5
21	3292.0
22	4018.0
23	4744.0
24	5069.5
25	5395.0
26	9399.0
27	13403.0
28	16194.0
29	18985.0
30	24154.0
31	29323.0
32	35317.0
33	41311.0
34	47941.0
35	54571.0
36	60840.0
37	67109.0
38	69727.5
39	82116.5
40	91887.0
41	98661.0
42	105435.0
43	113149.0
44	120863.0
45	137299.0
46	153735.0
47	176730.5
48	199726.0
49	214432.5
50	229139.0
51	215012.0
52	200885.0
53	182907.0
54	164929.0
55	156927.0
56	148925.0
57	137853.0
58	126781.0
59	122325.0
60	117869.0
61	108032.5
62	98196.0
63	90224.5
64	73513.0
65	64773.0
66	55479.5
67	46186.0
68	38151.5
69	30117.0
70	24896.5
71	19676.0
72	17543.0
73	15410.0
74	12208.0
75	9006.0
76	7227.0
77	5448.0
78	4326.5
79	3205.0
80	2302.0
81	1399.0
82	980.5
83	562.0
84	345.0
85	128.0
86	102.5
87	77.0
88	82.5
89	71.5
90	55.0
91	39.5
92	24.0
93	17.5
94	11.0
95	26.0
96	41.0
97	22.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2355588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.40607482612182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.42979270757066	12.259062418562236
2	11.761074114593	4.094282721483404
3	4.6263259260214085	2.415785257150679
4	2.340840625681024	1.629793883465189
5	1.4227303183273117	1.2382075189098654
6	0.8896562614271223	0.9291254073556972
7	0.6555907190308032	0.7987882777532818
8	0.48168072952310725	0.670733665630412
9	0.36882716360121676	0.5777849886794149
>10	3.734914551772437	15.402156252869482
>50	1.8827109348182467	24.058270718443467
>100	1.3980334144684663	33.30395798873616
>500	0.004400174905407642	0.506386053459491
>1k	0.002200087452703821	0.9523938353570108
>5k	0.0012222708070576784	1.1632710121442755
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	5915	0.25110503194955996	RNA PCR Primer, Index 27 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	5608	0.2380721925905549	RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	5453	0.23149209454284875	RNA PCR Primer, Index 38 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	5233	0.22215260053965294	RNA PCR Primer, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	5130	0.21778001925633855	RNA PCR Primer, Index 27 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4032	0.17116745373129766	RNA PCR Primer, Index 38 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3803	0.1614458895188802	RNA PCR Primer, Index 27 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3734	0.15851668458151424	RNA PCR Primer, Index 38 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3144	0.13346985975476186	RNA PCR Primer, Index 38 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2374	0.10078163074357656	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24291174857402908	0.0
2	0.0	0.0	0.0	0.6267224998599076	0.0
3	0.0	0.0	0.0	2.3906557513453115	0.0
4	0.0	0.0	0.0	2.775570261013386	0.0
5	0.0	0.0	0.0	3.429037675518809	0.0
6	4.245224546907184E-5	0.0	0.0	5.450571152510541	0.0
7	4.245224546907184E-5	0.0	0.0	5.696879080722096	0.0
8	4.245224546907184E-5	0.0	0.0	6.1805799655967	0.0
9	4.245224546907184E-5	0.0	0.0	6.48245788312727	0.0
10	4.245224546907184E-5	0.0	0.0	7.62089974987137	0.0
11	4.245224546907184E-5	0.0	0.0	8.523901463243996	0.0
12	4.245224546907184E-5	0.0	0.0	8.886231378322526	0.0
13	8.490449093814368E-5	0.0	0.0	9.071195811831272	0.0
14	8.490449093814368E-5	0.0	0.0	9.188194200344032	0.0
15	8.490449093814368E-5	0.0	0.0	9.357706016502037	0.0
16	8.490449093814368E-5	0.0	0.0	9.767455089769518	0.0
17	8.490449093814368E-5	0.0	0.0	10.305494848844535	0.0
18	8.490449093814368E-5	0.0	0.0	10.863572067780954	0.0
19	2.971657182835029E-4	0.0	0.0	11.223567109358683	0.0
20	2.971657182835029E-4	0.0	0.0	11.641933988456385	0.0
21	2.971657182835029E-4	0.0	0.0	12.28067047378404	0.0
22	2.971657182835029E-4	0.0	0.0	12.84604947894114	0.0
23	2.971657182835029E-4	0.0	0.0	13.424631132439119	0.0
24	2.971657182835029E-4	0.0	0.0	13.898016121664739	0.0
25	2.971657182835029E-4	0.0	0.0	14.29999643401138	0.0
26	2.971657182835029E-4	0.0	0.0	14.659694309870826	0.0
27	2.971657182835029E-4	0.0	0.0	15.041637162356066	0.0
28	2.971657182835029E-4	0.0	0.0	15.397811501841579	0.0
29	2.971657182835029E-4	0.0	0.0	15.81583876297553	0.0
30	2.971657182835029E-4	0.0	0.0	16.255516669298707	0.0
31	2.971657182835029E-4	0.0	0.0	16.606469382591523	0.0
32	2.971657182835029E-4	0.0	0.0	16.96429935965033	0.0
33	2.971657182835029E-4	0.0	0.0	17.342336605552415	0.0
34	2.971657182835029E-4	0.0	0.0	17.75832615890385	0.0
35	2.971657182835029E-4	0.0	0.0	18.211801045004474	0.0
36	2.971657182835029E-4	0.0	0.0	18.577569591965997	0.0
37	2.971657182835029E-4	0.0	0.0	18.961592604479222	0.0
38	2.971657182835029E-4	0.0	0.0	19.417826886535337	0.0
39	2.971657182835029E-4	0.0	0.0	20.149661146176665	0.0
40	2.971657182835029E-4	0.0	0.0	20.586876822262635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATT	45	3.110472E-10	46.000004	31
TAACGCG	20	6.3128106E-4	46.0	1
ATCCGAT	20	6.3128106E-4	46.0	20
GCTCGAA	25	3.418257E-5	46.0	19
ACGGGTA	160	0.0	43.125	5
TCGTTAG	165	0.0	41.81818	1
CCGTCAA	55	4.7293724E-11	41.81818	35
TAGTACG	50	8.731149E-10	41.399998	1
TACGTTG	45	1.5896148E-8	40.888893	1
TACGGGT	135	0.0	39.185184	4
TACTATT	2190	0.0	39.173515	46
ACGACCA	1820	0.0	38.670326	28
AACACGT	1775	0.0	38.48451	41
ACTACGC	30	1.0018856E-4	38.333332	30
TACTACG	30	1.0018856E-4	38.333332	29
TCGAATT	30	1.0018856E-4	38.333332	21
AAACACG	1790	0.0	38.03352	40
CGACCAA	1840	0.0	38.0	29
CACGACC	1880	0.0	37.925533	27
ATCGTAG	85	0.0	37.882355	1
>>END_MODULE