FastQCFastQC Report
Thu 26 May 2016
SRR1545620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545620_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2835817
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC98680.3479773201162134No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.2364045352714932No Hit
ATGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT50640.17857287688168877No Hit
ATGCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC42950.15145547120988412No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG42410.14955125806778083No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA41400.14598967422792092No Hit
ATTGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC39010.13756176791379698No Hit
ATGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC35430.1249375400457787No Hit
ATATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC33650.1186606893181048No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT32660.11516963189091539No Hit
ATGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC30550.10772909535417835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG1150.045.0000044
CGTATAG207.035039E-445.037
TCGACGA207.035039E-445.02
ACGTTAC253.8922604E-544.99999614
CGATGCA253.8922604E-544.99999631
TTACGAT556.184564E-1140.90909213
CTACGAT451.9299478E-840.037
CGTAAAC403.4606092E-739.37518
ATCCGTT2250.039.0000041
AACGGAT356.251532E-638.571436
CAATCGA356.251532E-638.5714344
AATCGAC356.251532E-638.5714345
GCTACGA2050.038.4146310
ACGGGTA1650.038.1818167
TCGAATC301.1403441E-437.50000416
ACGGTAG301.1403441E-437.50000427
ATCGTAC900.037.51
ATCGGGT2350.037.3404271
ATTCGTT6850.037.1167871
TACGTAG850.037.0588233