FastQCFastQC Report
Thu 26 May 2016
SRR1545619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545619_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2294022
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT76120.33181896250341103No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT75040.327111073912979Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC69670.30370240564388656No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC69570.3032664900336614No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC63950.27876803273900597No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC51700.2253683704864208No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC48900.21316273340011563No Hit
TTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC48000.20923949290808896No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC36070.15723476060822433No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC29500.12859510501642965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC351.020162E-746.00000423
CGAAACG253.418228E-546.046
CGTACGT301.8622195E-646.029
ATAACGG600.046.02
CAGTCGA206.312775E-446.010
CGCGTTA301.8622195E-646.040
ATACGAC206.312775E-446.016
CAATCGA206.312775E-446.042
TAGTCGA253.418228E-546.010
TACGTAG800.043.1251
TAAATCG650.042.461541
ATACTCG950.041.15789444
TTGTTCG1800.040.888891
CGTAATC402.9122748E-740.2534
TAATACG355.375454E-639.42857410
GCGAATA355.375454E-639.4285749
TCAGACG5450.038.40366722
CGAACCG301.00187724E-438.33333238
CGTATGA301.00187724E-438.33333214
ACGATTT301.00187724E-438.33333220