##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545619_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2294022
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522580428609665	33.0	31.0	34.0	31.0	34.0
2	32.935812298225564	34.0	31.0	34.0	31.0	34.0
3	33.013908759375454	34.0	33.0	34.0	31.0	34.0
4	36.40055980282665	37.0	37.0	37.0	35.0	37.0
5	36.376764041495676	37.0	37.0	37.0	35.0	37.0
6	36.38113104407891	37.0	37.0	37.0	35.0	37.0
7	36.46247420469376	37.0	37.0	37.0	35.0	37.0
8	36.49295516782315	37.0	37.0	37.0	35.0	37.0
9	38.284976342859835	39.0	39.0	39.0	37.0	39.0
10	38.05812585929865	39.0	39.0	39.0	35.0	39.0
11	37.884960998630355	39.0	38.0	39.0	35.0	39.0
12	37.54881862510473	39.0	37.0	39.0	35.0	39.0
13	37.483552904026205	39.0	37.0	39.0	35.0	39.0
14	38.69911971201671	41.0	38.0	41.0	35.0	41.0
15	38.7950956878356	41.0	38.0	41.0	35.0	41.0
16	38.890893810085515	41.0	38.0	41.0	35.0	41.0
17	38.834344221633444	41.0	38.0	41.0	35.0	41.0
18	38.82475495003971	40.0	38.0	41.0	35.0	41.0
19	38.801174966935804	40.0	38.0	41.0	35.0	41.0
20	38.678749375550886	40.0	38.0	41.0	35.0	41.0
21	38.66175041041455	40.0	38.0	41.0	35.0	41.0
22	38.77997072390762	40.0	38.0	41.0	35.0	41.0
23	38.77948075476172	40.0	38.0	41.0	35.0	41.0
24	38.73752649277121	40.0	38.0	41.0	35.0	41.0
25	38.66794956630756	40.0	38.0	41.0	35.0	41.0
26	38.556903551927576	40.0	37.0	41.0	35.0	41.0
27	38.488023218609065	40.0	37.0	41.0	35.0	41.0
28	38.43839597004737	40.0	37.0	41.0	35.0	41.0
29	38.38154690757107	40.0	37.0	41.0	35.0	41.0
30	38.30973896501428	40.0	37.0	41.0	34.0	41.0
31	38.233605431857235	40.0	36.0	41.0	34.0	41.0
32	38.19331941890705	40.0	36.0	41.0	34.0	41.0
33	38.09404661332803	40.0	36.0	41.0	34.0	41.0
34	38.00140190460248	40.0	36.0	41.0	34.0	41.0
35	37.92037434688944	40.0	36.0	41.0	34.0	41.0
36	37.82573270875345	40.0	35.0	41.0	34.0	41.0
37	37.761599060514676	40.0	35.0	41.0	34.0	41.0
38	37.655453609424846	40.0	35.0	41.0	33.0	41.0
39	37.53753843685893	40.0	35.0	41.0	33.0	41.0
40	37.38017464523008	40.0	35.0	41.0	33.0	41.0
41	37.23917076645298	39.0	35.0	41.0	33.0	41.0
42	37.186987744668535	39.0	35.0	41.0	33.0	41.0
43	37.10814150866906	39.0	35.0	41.0	33.0	41.0
44	36.9805686257586	39.0	35.0	41.0	33.0	41.0
45	36.944021896912936	39.0	35.0	41.0	33.0	41.0
46	36.928674616023734	39.0	35.0	41.0	33.0	41.0
47	36.88825259740317	39.0	35.0	41.0	33.0	41.0
48	36.78089704457935	38.0	35.0	41.0	32.0	41.0
49	36.71300798335849	38.0	35.0	41.0	32.0	41.0
50	36.62060825920588	38.0	35.0	41.0	32.0	41.0
51	36.49144297657128	38.0	35.0	40.0	32.0	41.0
52	36.15615543355731	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	6.0
11	6.0
12	21.0
13	25.0
14	34.0
15	57.0
16	95.0
17	208.0
18	450.0
19	714.0
20	1229.0
21	2079.0
22	2864.0
23	3995.0
24	5326.0
25	6918.0
26	8922.0
27	11218.0
28	14191.0
29	18264.0
30	23391.0
31	30484.0
32	40072.0
33	57380.0
34	131832.0
35	207633.0
36	141913.0
37	215183.0
38	353603.0
39	1014371.0
40	1532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.708878990698434	13.689798964438877	45.34389818406275	12.25742386079994
2	26.6980874638517	13.063562598789375	48.034630879738735	12.203719057620198
3	26.710816199670273	13.890494511386553	43.92167991414206	15.477009374801112
4	24.086211902065454	14.623573793102246	45.41817820404512	15.872036100787176
5	24.430498051021306	16.48083584202767	43.14378850769522	15.944877599255806
6	26.007466362571936	18.93116107866446	42.83999891892928	12.221373639834317
7	85.7751146240097	2.857383233465067	6.830536062862519	4.536966079662706
8	86.45357367976419	3.107773159978413	7.071074296584776	3.367578863672624
9	78.50029337120567	5.07118065999367	9.42353647872601	7.004989490074637
10	43.456165633982586	22.049265438605207	15.072261730707028	19.422307196705173
11	34.01048464225714	22.793242610576534	24.93110353780391	18.265169209362423
12	28.291446202346794	23.444631306936028	27.093768063253098	21.170154427464077
13	24.273132515730016	22.775195704313212	34.17460686950692	18.777064910449855
14	19.67910508268883	29.65468509020402	31.3242854689275	19.341924358179654
15	19.41733775874861	22.835439241646334	36.05915723563244	21.688065763972624
16	24.72478467948433	22.08871580133059	31.57628828319868	21.610211235986405
17	24.87731155150212	21.795736919698243	27.204621402933366	26.122330125866274
18	25.137378804562466	22.462513437098686	28.793010703471893	23.607097054866955
19	26.594688281106283	24.588604642849983	27.093201372959808	21.72350570308393
20	26.642682589792077	23.19785947998755	26.01234861740646	24.147109312813914
21	26.748261350588614	24.39645304186272	28.059539097707	20.795746509841667
22	29.99513518178989	20.049894900746374	26.46099296345022	23.49397695401352
23	28.318996068913027	23.47998406292529	23.856353600793714	24.344666267367966
24	27.358543204903874	21.333317640371366	27.035573329288038	24.272565825436722
25	29.743175959079732	22.36879158090027	23.730679130365793	24.157353329654203
26	23.16268109024238	25.418544373157715	24.870947183592833	26.547827353007076
27	22.526375073996675	22.24751985813562	26.601968071797046	28.62413699607066
28	23.45683694402233	22.517395212426035	29.540649566569112	24.485118276982522
29	23.03949133879274	22.407718844893378	28.593099804622625	25.959690011691254
30	23.54293027704181	23.489051107617975	28.677449475201193	24.290569140139024
31	23.990223284693872	25.147404863597643	27.56926481088673	23.293107040821752
32	26.20149240068317	22.764995279033943	25.50332995934651	25.530182360936383
33	25.216846220306515	22.516349014961495	26.70096450687918	25.565840257852802
34	21.628083776005635	24.904338319336084	28.533771690070974	24.933806214587307
35	22.364040100748817	22.491763374544796	29.113234310743312	26.030962213963075
36	24.54789012485495	23.195156803204153	26.64826230960296	25.608690762337936
37	24.445144815524873	24.312059779723125	28.63939404242854	22.603401362323467
38	23.424056090133398	24.129847054648994	29.015109706881624	23.43098714833598
39	22.98565576092993	25.492475660651902	24.88594268058458	26.635925897833584
40	25.599623717645255	21.785274945052837	27.4236254055105	25.191475931791413
41	22.807148318542716	21.56770946398945	28.178936383347676	27.446205834120164
42	21.562434885105723	23.37301037217603	27.703701185080178	27.36085355763807
43	22.132176587670042	20.514973265295623	29.568766123428635	27.784084023605704
44	25.851364982550294	21.207120071211175	27.53395564645849	25.407559299780036
45	21.969972389105248	21.90820314713634	28.10195368658191	28.019870777176504
46	21.592731020016373	22.7995633869248	27.076592988210223	28.5311126048486
47	22.590759809626935	22.024069516334194	31.13963161643611	24.245539057602763
48	21.35934180230181	21.821237982896417	32.554744461910126	24.264675752891645
49	21.42599329910524	21.502801629626916	28.493929003296394	28.577276067971447
50	20.613446601645492	22.10881151096197	31.01448024474046	26.263261642652076
51	23.066212965699545	22.667655323270658	27.87954954224502	26.386582168784777
52	21.784272339149318	21.424859918518653	28.74619336693371	28.044674375398316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	202.0
1	167.5
2	133.0
3	123.5
4	114.0
5	186.5
6	259.0
7	417.5
8	576.0
9	744.5
10	913.0
11	979.0
12	1045.0
13	1199.5
14	1405.0
15	1456.0
16	1548.0
17	1640.0
18	2200.0
19	2760.0
20	3052.0
21	3344.0
22	3924.0
23	4504.0
24	5124.5
25	5745.0
26	8290.0
27	10835.0
28	13314.0
29	15793.0
30	20494.0
31	25195.0
32	28242.0
33	31289.0
34	38691.5
35	46094.0
36	48515.0
37	50936.0
38	58659.0
39	77329.5
40	88277.0
41	103264.5
42	118252.0
43	134602.5
44	150953.0
45	180917.0
46	210881.0
47	224093.0
48	237305.0
49	212866.0
50	188427.0
51	170134.0
52	151841.0
53	145948.5
54	140056.0
55	135793.0
56	131530.0
57	129102.5
58	126675.0
59	120184.5
60	113694.0
61	106118.5
62	98543.0
63	89433.0
64	69761.5
65	59200.0
66	51432.0
67	43664.0
68	37216.5
69	30769.0
70	25768.0
71	20767.0
72	17780.0
73	14793.0
74	11295.5
75	7798.0
76	6082.0
77	4366.0
78	3520.5
79	2675.0
80	1973.0
81	1271.0
82	1037.5
83	804.0
84	539.0
85	274.0
86	206.0
87	138.0
88	126.5
89	69.5
90	24.0
91	19.5
92	15.0
93	12.5
94	10.0
95	9.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2294022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.51813631992958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26394943398594	12.13375308241275
2	12.231100302494319	4.285321648836548
3	4.9930648848794545	2.6240757392271536
4	2.639052069581329	1.84925095641272
5	1.611643688478345	1.4116496916958883
6	1.0391411085182976	1.0922289356799764
7	0.7750063113651501	0.9503666347910342
8	0.5382461666202203	0.754325577642604
9	0.40893334950930693	0.6447375146222502
>10	3.52300891233439	13.71330488431923
>50	1.4615288924741965	18.828706427928502
>100	1.5050946280301885	38.02163124833073
>500	0.005489403366682258	0.6350765675096721
>1k	0.003243738353039516	1.2806874768714904
>5k	0.0014971100090951613	1.774883613719474
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	7612	0.33181896250341103	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	7504	0.327111073912979	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	6967	0.30370240564388656	No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	6957	0.3032664900336614	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	6395	0.27876803273900597	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	5170	0.2253683704864208	No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	4890	0.21316273340011563	No Hit
TTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	4800	0.20923949290808896	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	3607	0.15723476060822433	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2950	0.12859510501642965	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34075523251302736	0.0
2	0.0	0.0	0.0	0.8643334719544974	0.0
3	0.0	0.0	0.0	3.1523237353434275	0.0
4	0.0	0.0	0.0	3.662170633062804	0.0
5	0.0	0.0	0.0	4.487838390390327	0.0
6	0.0	0.0	0.0	7.01126667486188	0.0
7	0.0	0.0	0.0	7.328046548812522	0.0
8	0.0	0.0	0.0	7.927474104433174	0.0
9	0.0	0.0	0.0	8.322326464175147	0.0
10	0.0	0.0	0.0	9.664205487131335	0.0
11	0.0	0.0	0.0	10.778362195305887	0.0
12	0.0	0.0	0.0	11.231016964963718	0.0
13	0.0	0.0	0.0	11.471337240880864	0.0
14	0.0	0.0	0.0	11.612748264837913	0.0
15	4.359156102251853E-5	0.0	0.0	11.842301425182496	0.0
16	4.359156102251853E-5	0.0	0.0	12.334057825077528	0.0
17	4.359156102251853E-5	0.0	0.0	12.96879454512642	0.0
18	4.359156102251853E-5	0.0	0.0	13.64001740175116	0.0
19	4.359156102251853E-5	0.0	0.0	14.112070416064013	0.0
20	4.359156102251853E-5	0.0	0.0	14.610627099478558	0.0
21	4.359156102251853E-5	0.0	0.0	15.347455255442188	0.0
22	4.359156102251853E-5	0.0	0.0	15.987553737496851	0.0
23	8.718312204503706E-5	0.0	0.0	16.619587780762348	0.0
24	8.718312204503706E-5	0.0	0.0	17.11810087261587	0.0
25	8.718312204503706E-5	0.0	0.0	17.56399895031521	0.0
26	8.718312204503706E-5	0.0	0.0	17.964082297379885	0.0
27	8.718312204503706E-5	0.0	0.0	18.385220368418437	0.0
28	8.718312204503706E-5	0.0	0.0	18.783429278359144	0.0
29	8.718312204503706E-5	0.0	0.0	19.246153698613178	0.0
30	8.718312204503706E-5	0.0	0.0	19.690351705432644	0.0
31	8.718312204503706E-5	0.0	0.0	20.106084422904402	0.0
32	8.718312204503706E-5	0.0	0.0	20.536987003611998	0.0
33	8.718312204503706E-5	0.0	0.0	20.96387916070552	0.0
34	8.718312204503706E-5	0.0	0.0	21.428216468717387	0.0
35	8.718312204503706E-5	0.0	0.0	21.90340807542386	0.0
36	1.307746830675556E-4	0.0	0.0	22.31617656674609	0.0
37	1.307746830675556E-4	0.0	0.0	22.768874927964944	0.0
38	1.307746830675556E-4	0.0	0.0	23.275844782656836	0.0
39	1.7436624409007413E-4	0.0	0.0	24.02566322380518	0.0
40	1.7436624409007413E-4	0.0	0.0	24.489303066840684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTC	35	1.020162E-7	46.000004	23
CGAAACG	25	3.418228E-5	46.0	46
CGTACGT	30	1.8622195E-6	46.0	29
ATAACGG	60	0.0	46.0	2
CAGTCGA	20	6.312775E-4	46.0	10
CGCGTTA	30	1.8622195E-6	46.0	40
ATACGAC	20	6.312775E-4	46.0	16
CAATCGA	20	6.312775E-4	46.0	42
TAGTCGA	25	3.418228E-5	46.0	10
TACGTAG	80	0.0	43.125	1
TAAATCG	65	0.0	42.46154	1
ATACTCG	95	0.0	41.157894	44
TTGTTCG	180	0.0	40.88889	1
CGTAATC	40	2.9122748E-7	40.25	34
TAATACG	35	5.375454E-6	39.428574	10
GCGAATA	35	5.375454E-6	39.428574	9
TCAGACG	545	0.0	38.403667	22
CGAACCG	30	1.00187724E-4	38.333332	38
CGTATGA	30	1.00187724E-4	38.333332	14
ACGATTT	30	1.00187724E-4	38.333332	20
>>END_MODULE