FastQCFastQC Report
Thu 26 May 2016
SRR1545618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545618_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2493516
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC85820.3441726461751198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57700.23140015945355874No Hit
ATGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT38760.15544315737296252No Hit
ATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG36580.14670048237107763No Hit
ATTGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC31900.1279318039266642No Hit
ATGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC31740.12729013970634237No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT28940.1160610158507104No Hit
ATGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC28460.11413602318974493No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26880.10779958901406689No Hit
ATATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC26870.10775948500029676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG453.8562575E-1045.00000434
CCGATAG453.8562575E-1045.00000413
ATTACGC1400.045.0000041
CCCGTAA351.212702E-745.00000418
CGTAAGT351.212702E-745.00000420
CTAGCGA453.8562575E-1045.00000416
CTATGCG207.0348755E-445.020
CGTATCA207.0348755E-445.040
CGAGTTA253.8921236E-545.08
CACTCGA207.0348755E-445.031
CGGATTA253.8921236E-545.08
GTATCGC207.0348755E-445.010
ACGTATC207.0348755E-445.039
ACGTAAG253.8921236E-545.038
CGGTCCA406.8212103E-945.014
TCGACGA207.0348755E-445.030
ATACCCG207.0348755E-445.045
CGTATTA302.1665273E-644.99999630
CTAAGCG302.1665273E-644.99999645
CGATCAA302.1665273E-644.99999642