##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545618_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2493516
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88148461850656	31.0	31.0	34.0	31.0	34.0
2	32.54382245792688	34.0	31.0	34.0	31.0	34.0
3	32.50787723038473	34.0	31.0	34.0	31.0	34.0
4	35.98713703862337	37.0	35.0	37.0	35.0	37.0
5	35.75573647812968	37.0	35.0	37.0	35.0	37.0
6	35.75837732743644	37.0	35.0	37.0	35.0	37.0
7	35.73971251838769	37.0	35.0	37.0	33.0	37.0
8	35.707573963832594	37.0	35.0	37.0	33.0	37.0
9	37.894057627863624	39.0	38.0	39.0	35.0	39.0
10	37.8261559179889	39.0	38.0	39.0	35.0	39.0
11	37.64258220119702	39.0	37.0	39.0	35.0	39.0
12	37.148946307142204	39.0	37.0	39.0	33.0	39.0
13	36.9324592262492	39.0	37.0	39.0	33.0	39.0
14	37.71832504784409	40.0	36.0	41.0	33.0	41.0
15	37.60637589652523	40.0	36.0	41.0	33.0	41.0
16	37.608132853368495	40.0	36.0	41.0	32.0	41.0
17	37.67285952847305	40.0	36.0	41.0	33.0	41.0
18	37.7265223082587	40.0	36.0	41.0	33.0	41.0
19	37.69228711586371	40.0	35.0	41.0	33.0	41.0
20	37.64895914042661	40.0	36.0	41.0	33.0	41.0
21	37.64394052414342	40.0	36.0	41.0	33.0	41.0
22	37.59111270992446	40.0	35.0	41.0	32.0	41.0
23	37.53116603222117	40.0	35.0	41.0	32.0	41.0
24	37.48384449909285	40.0	35.0	41.0	32.0	41.0
25	37.41384735449863	39.0	35.0	41.0	32.0	41.0
26	37.30703873566482	39.0	35.0	41.0	32.0	41.0
27	37.220253248826154	39.0	35.0	41.0	32.0	41.0
28	37.11499665532525	39.0	35.0	41.0	32.0	41.0
29	37.05466257284894	39.0	35.0	41.0	31.0	41.0
30	36.98893089115931	39.0	35.0	41.0	31.0	41.0
31	36.93545459503769	39.0	35.0	41.0	31.0	41.0
32	36.82623612601644	39.0	35.0	41.0	31.0	41.0
33	36.7249799078891	39.0	35.0	41.0	31.0	41.0
34	36.640307100495846	39.0	35.0	41.0	30.0	41.0
35	36.54358664632591	39.0	35.0	41.0	30.0	41.0
36	36.4440007603721	39.0	35.0	41.0	30.0	41.0
37	36.274658353906695	38.0	35.0	41.0	30.0	41.0
38	36.22167413403403	38.0	35.0	41.0	30.0	41.0
39	36.16699872790068	38.0	35.0	41.0	30.0	41.0
40	36.0905195715608	38.0	35.0	41.0	30.0	41.0
41	36.03164728038641	38.0	35.0	40.0	30.0	41.0
42	35.88028631057511	38.0	35.0	40.0	29.0	41.0
43	35.76369712486304	38.0	35.0	40.0	29.0	41.0
44	35.69001161412239	38.0	35.0	40.0	29.0	41.0
45	35.65358874777623	38.0	35.0	40.0	29.0	41.0
46	35.60020348776587	38.0	34.0	40.0	29.0	41.0
47	35.5046580811994	37.0	34.0	40.0	28.0	41.0
48	35.41778115720934	37.0	34.0	40.0	28.0	41.0
49	35.37043516063262	37.0	34.0	40.0	28.0	41.0
50	35.272485919480765	37.0	34.0	40.0	28.0	41.0
51	35.10429449821056	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	11.0
10	18.0
11	35.0
12	27.0
13	29.0
14	50.0
15	72.0
16	138.0
17	289.0
18	659.0
19	1313.0
20	2314.0
21	3830.0
22	5945.0
23	8994.0
24	12611.0
25	17947.0
26	24010.0
27	29720.0
28	35125.0
29	41848.0
30	49897.0
31	61494.0
32	76458.0
33	97872.0
34	155891.0
35	282445.0
36	186681.0
37	250315.0
38	381274.0
39	765770.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.2735767486553127	90.84637916901275	3.306936871469844	3.573107210862092
2	3.50918141291253	2.8850426466082433	90.53031943649049	3.0754565039887454
3	28.52149334513995	15.9756745094076	42.51743321478587	12.985398930666578
4	27.867757816673322	15.69169798790142	42.38083894388486	14.059705251540395
5	25.180107125841584	14.071936975740279	46.210331114779294	14.537624783638844
6	23.235102562004815	14.606804207392294	47.21128719446757	14.946806036135321
7	23.048899626070178	17.37325928528231	43.852054689041495	15.725786399606017
8	25.805288596503896	19.844829549920675	42.49970724069948	11.850174612875955
9	81.87896127396014	4.447735647174512	9.123221988549501	4.550081090315843
10	83.23142903434348	4.539253006597913	8.557996018473514	3.6713219405851016
11	76.49808543438262	6.963981783152786	9.836231249368362	6.701701533096238
12	41.38902657933617	24.934389833472093	16.227607923911457	17.448975663280283
13	33.18887867573338	23.971412254824113	24.291161556613233	18.548547512829273
14	29.91382449521078	22.852750894720547	27.411053307859262	19.82237130220941
15	22.519967788456142	27.26663875427308	31.049570165180413	19.163823292090363
16	19.12668697533924	32.31870178494945	28.933241254517718	19.6213699851936
17	18.555244883128882	24.39555230445684	37.46288373525576	19.586319077158517
18	21.215223804459246	21.282117299427796	36.117875321433665	21.38478357467929
19	21.063710840435753	21.52490699879207	30.120039334016706	27.29134282675547
20	22.51074386528901	22.929309457007697	31.76574764308711	22.79419903461618
21	25.758366900392858	25.131982309317447	27.279832974803455	21.829817815486244
22	27.538543967634453	22.356142892205224	27.322944789606325	22.782368350554
23	25.426385874403856	24.98560265905653	27.629459766851305	21.95855169968831
24	27.741911421462706	22.67136044043832	25.060476852765333	24.526251285333643
25	26.38246556268338	24.674916864379455	24.467338489105344	24.47527908383183
26	26.21888129051508	21.19244472463782	30.61379995155435	21.97487403329275
27	26.54167849735073	23.986451259987902	26.098007793011956	23.373862449649412
28	23.143785722650264	27.158678749203936	25.233445464155835	24.464090063989964
29	21.33324991698469	26.86611996875095	26.43776899767236	25.362861116591993
30	20.934455604054676	26.617475083376245	29.510779156821133	22.937290155747945
31	21.53585539455131	24.53683874496895	27.74804733556953	26.17925852491021
32	22.43157854210681	25.62578303086886	28.00735988860709	23.93527853841724
33	24.505637822255803	26.935058768421776	25.229836102916526	23.329467306405892
34	26.12002489657175	24.504354493815157	25.16743425749023	24.208186352122866
35	25.06003570861386	26.203361037186045	25.16554936884303	23.571053885357063
36	23.51703377880872	23.95220243222823	27.14841212167879	25.38235166728427
37	22.207517417173182	24.869621851233358	27.88039058101091	25.042470150582552
38	24.41993554482907	27.742111941531554	23.31101946007164	24.526933053567735
39	24.906036295736623	28.660253232784548	25.302263951785353	21.13144651969348
40	23.437748143585203	25.83304057403281	27.610330152282963	23.118881130099027
41	22.487684057371197	26.726437688789645	25.66849380553403	25.117384448305124
42	24.584562521355387	24.030325051052408	27.66286641032181	23.722246017270393
43	21.8289355271833	24.79262214479474	27.485807189526756	25.8926351384952
44	21.926709112754843	26.273101917132273	25.157087421937536	26.643101548175345
45	21.821756908718452	24.324407784028658	26.6732196625167	27.18061564473619
46	23.74586728138099	24.907439936218577	26.241900994419126	25.104791787981306
47	21.62191860810197	25.998630046889616	26.40576599468381	25.973685350324605
48	21.496272732960207	27.117612239103337	25.093442352084367	26.292672675852092
49	21.9859427410933	25.85413528527589	28.035432698246172	24.124489275384636
50	21.336899382237775	22.907091833379052	30.35978112833445	25.396227656048726
51	20.729203261579233	23.90014742235462	28.525704266585816	26.844945049480334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	584.0
1	1572.0
2	2560.0
3	6887.5
4	11215.0
5	7834.5
6	4454.0
7	4418.0
8	4382.0
9	4584.0
10	4786.0
11	5030.0
12	5274.0
13	5280.5
14	5287.0
15	5550.5
16	5814.0
17	5970.5
18	6127.0
19	6654.0
20	7181.0
21	7798.0
22	8415.0
23	9187.5
24	9960.0
25	13266.5
26	20304.5
27	24036.0
28	27773.5
29	31511.0
30	40959.0
31	50407.0
32	52895.5
33	55384.0
34	63369.0
35	71354.0
36	83044.5
37	94735.0
38	105370.5
39	116006.0
40	132340.0
41	148674.0
42	160424.0
43	172174.0
44	178304.0
45	184434.0
46	198997.5
47	213561.0
48	220485.0
49	227409.0
50	209645.5
51	191882.0
52	169213.5
53	146545.0
54	141424.0
55	136303.0
56	138394.5
57	140486.0
58	133826.0
59	127166.0
60	109430.5
61	91695.0
62	77896.5
63	64098.0
64	53239.5
65	42381.0
66	33804.0
67	25227.0
68	22075.5
69	18924.0
70	15738.0
71	12552.0
72	10023.0
73	7494.0
74	5424.0
75	2416.5
76	1479.0
77	1152.0
78	825.0
79	599.0
80	373.0
81	273.5
82	174.0
83	121.5
84	69.0
85	96.5
86	124.0
87	79.5
88	35.0
89	22.0
90	9.0
91	15.0
92	21.0
93	10.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2493516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.338635946956506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0744508904282	11.055237632850732
2	10.773199895026107	3.30492382347191
3	4.5023600081319515	2.071801832006164
4	2.6508444561188242	1.6264135225765828
5	1.623855777127959	1.2453866297863954
6	1.100352657467519	1.0126745295696242
7	0.8149015765242718	0.8749635030434704
8	0.5817056943336645	0.7138057498924512
9	0.44827410976505044	0.6188322036718915
>10	2.684867892784465	8.416080720244647
>50	0.8541503740010957	9.75553378691523
>100	1.8715766886877812	55.41635842701736
>500	0.013148634867010843	1.371720794259654
>1k	0.005785399341484771	1.937358477616114
>5k	5.259453946804337E-4	0.5789083670777944
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	8582	0.3441726461751198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5770	0.23140015945355874	No Hit
ATGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	3876	0.15544315737296252	No Hit
ATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	3658	0.14670048237107763	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	3190	0.1279318039266642	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	3174	0.12729013970634237	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	2894	0.1160610158507104	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	2846	0.11413602318974493	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2688	0.10779958901406689	No Hit
ATATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	2687	0.10775948500029676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0104013770114166E-5	0.0	0.0	0.13298490966169857	0.0
2	4.0104013770114166E-5	0.0	0.0	0.6355684102287693	0.0
3	4.0104013770114166E-5	0.0	0.0	0.8611935916994317	0.0
4	8.020802754022833E-5	0.0	0.0	1.1642997277739546	0.0
5	1.203120413103425E-4	0.0	0.0	2.3575144494761613	0.0
6	1.203120413103425E-4	0.0	0.0	2.6196743874913975	0.0
7	1.203120413103425E-4	0.0	0.0	3.0548430409109066	0.0
8	1.203120413103425E-4	0.0	0.0	4.404503520330329	0.0
9	1.203120413103425E-4	0.0	0.0	4.5641977031629235	0.0
10	1.203120413103425E-4	0.0	0.0	4.8800569156163425	0.0
11	1.203120413103425E-4	0.0	0.0	5.1216835985812805	0.0
12	2.0052006885057085E-4	0.0	0.0	5.837620452405359	0.0
13	2.0052006885057085E-4	0.0	0.0	6.4706623097666105	0.0
14	2.40624082620685E-4	0.0	0.0	6.7163796021361	0.0
15	2.40624082620685E-4	0.0	0.0	6.861796756066534	0.0
16	2.40624082620685E-4	0.0	0.0	6.963861471111475	0.0
17	2.40624082620685E-4	0.0	0.0	7.096044300497771	0.0
18	2.40624082620685E-4	0.0	0.0	7.39421764287857	0.0
19	2.8072809639079915E-4	0.0	0.0	7.786595313605367	0.0
20	2.8072809639079915E-4	0.0	0.0	8.213342124133153	0.0
21	3.609361239310275E-4	0.0	0.0	8.486851498045331	0.0
22	4.010401377011417E-4	0.0	0.0	8.776242061410475	0.0
23	4.4114415147125587E-4	0.0	0.0	9.231302305659959	0.0
24	4.4114415147125587E-4	0.0	0.0	9.637154925013515	0.0
25	5.213521790114842E-4	0.0	0.0	10.06763140882192	0.0
26	5.614561927815983E-4	0.0	0.0	10.421589434356948	0.0
27	6.416642203218267E-4	0.0	0.0	10.728545555753403	0.0
28	6.416642203218267E-4	0.0	0.0	11.010958020722546	0.0
29	6.817682340919408E-4	0.0	0.0	11.267543500823736	0.0
30	6.817682340919408E-4	0.0	0.0	11.530826351224537	0.0
31	7.21872247862055E-4	0.0	0.0	11.827034596930599	0.0
32	7.619762616321692E-4	0.0	0.0	12.152719292757697	0.0
33	8.020802754022834E-4	0.0	0.0	12.465490496150817	0.0
34	8.020802754022834E-4	0.0	0.0	12.768235696101408	0.0
35	8.421842891723976E-4	0.0	0.0	13.070700167955609	0.0
36	8.421842891723976E-4	0.0	0.0	13.3800224261645	0.0
37	8.822883029425117E-4	0.0	0.0	13.739915845737505	0.0
38	9.223923167126259E-4	0.0	0.0	14.066603141908855	0.0
39	0.0010026003442528543	0.0	0.0	14.389320140716963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	45	3.8562575E-10	45.000004	34
CCGATAG	45	3.8562575E-10	45.000004	13
ATTACGC	140	0.0	45.000004	1
CCCGTAA	35	1.212702E-7	45.000004	18
CGTAAGT	35	1.212702E-7	45.000004	20
CTAGCGA	45	3.8562575E-10	45.000004	16
CTATGCG	20	7.0348755E-4	45.0	20
CGTATCA	20	7.0348755E-4	45.0	40
CGAGTTA	25	3.8921236E-5	45.0	8
CACTCGA	20	7.0348755E-4	45.0	31
CGGATTA	25	3.8921236E-5	45.0	8
GTATCGC	20	7.0348755E-4	45.0	10
ACGTATC	20	7.0348755E-4	45.0	39
ACGTAAG	25	3.8921236E-5	45.0	38
CGGTCCA	40	6.8212103E-9	45.0	14
TCGACGA	20	7.0348755E-4	45.0	30
ATACCCG	20	7.0348755E-4	45.0	45
CGTATTA	30	2.1665273E-6	44.999996	30
CTAAGCG	30	2.1665273E-6	44.999996	45
CGATCAA	30	2.1665273E-6	44.999996	42
>>END_MODULE