FastQCFastQC Report
Thu 26 May 2016
SRR1545617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545617_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2044901
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT69460.3396741455943344Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT63610.3110664037036512No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC61120.29888977510402703No Hit
GTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC60550.2961023540992938No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC52630.2573718727703689No Hit
TTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC49220.24069624886485946No Hit
ATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC42990.2102302263043541No Hit
GGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC40720.1991294444083112No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC36330.17766141245957628No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC28810.1408870160462536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA301.8621195E-646.040
ACACGTT206.3126144E-446.045
CTCCGAT206.3126144E-446.041
TCCGATT453.110472E-1046.024
TCCGATG253.4180972E-546.019
ACGTTAC405.6152203E-946.013
GACCGTA301.8621195E-646.038
GCTATCG206.3126144E-446.030
CCAATCG301.8621195E-646.021
CGATTCG206.3126144E-446.016
TCTAGCG650.046.01
CTGCGTA206.3126144E-446.01
CGTTACA453.110472E-1046.037
CGTAGCG253.4180972E-546.01
CTACGAT206.3126144E-446.027
CACTACG453.110472E-1046.018
TACGAAC206.3126144E-446.034
GTCGTTA206.3126144E-446.035
GTCGTAA206.3126144E-446.031
CAAATCG206.3126144E-446.023
Produced by FastQC (version 0.11.5)