##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545617_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2044901
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.533046832096026	33.0	31.0	34.0	31.0	34.0
2	32.942825104980635	34.0	31.0	34.0	31.0	34.0
3	33.021028890885184	34.0	33.0	34.0	31.0	34.0
4	36.40252364295387	37.0	37.0	37.0	35.0	37.0
5	36.38437264200076	37.0	37.0	37.0	35.0	37.0
6	36.386101821066156	37.0	37.0	37.0	35.0	37.0
7	36.47567192739404	37.0	37.0	37.0	35.0	37.0
8	36.5122878809292	37.0	37.0	37.0	35.0	37.0
9	38.31378829586371	39.0	39.0	39.0	37.0	39.0
10	38.05684529471109	39.0	39.0	39.0	35.0	39.0
11	37.907335367335634	39.0	38.0	39.0	35.0	39.0
12	37.576727186303884	39.0	37.0	39.0	35.0	39.0
13	37.5018883554754	39.0	37.0	39.0	35.0	39.0
14	38.81645321705061	41.0	38.0	41.0	35.0	41.0
15	38.844658494469904	41.0	38.0	41.0	35.0	41.0
16	38.89817013146358	41.0	38.0	41.0	35.0	41.0
17	38.84670162516425	41.0	38.0	41.0	35.0	41.0
18	38.83116150855225	41.0	38.0	41.0	35.0	41.0
19	38.81877851299403	40.0	38.0	41.0	35.0	41.0
20	38.76346238766571	40.0	38.0	41.0	35.0	41.0
21	38.719989378458905	40.0	38.0	41.0	35.0	41.0
22	38.80553679615786	41.0	38.0	41.0	35.0	41.0
23	38.792172824014465	41.0	38.0	41.0	35.0	41.0
24	38.75019768683178	41.0	38.0	41.0	35.0	41.0
25	38.664828272860156	40.0	37.0	41.0	35.0	41.0
26	38.541983695054185	40.0	37.0	41.0	35.0	41.0
27	38.46279746550078	40.0	37.0	41.0	35.0	41.0
28	38.41966774919666	40.0	37.0	41.0	35.0	41.0
29	38.403352533936854	40.0	37.0	41.0	35.0	41.0
30	38.353632278530846	40.0	36.0	41.0	35.0	41.0
31	38.297911243624995	40.0	36.0	41.0	34.0	41.0
32	38.23626131533996	40.0	36.0	41.0	34.0	41.0
33	38.176131754055575	40.0	36.0	41.0	34.0	41.0
34	38.06167535738894	40.0	36.0	41.0	34.0	41.0
35	37.93246470122514	40.0	35.0	41.0	34.0	41.0
36	37.902022151683624	40.0	35.0	41.0	34.0	41.0
37	37.82534704614062	40.0	35.0	41.0	34.0	41.0
38	37.73313622517667	40.0	35.0	41.0	34.0	41.0
39	37.67374704203284	40.0	35.0	41.0	33.0	41.0
40	37.52983494066461	40.0	35.0	41.0	33.0	41.0
41	37.40055533250754	39.0	35.0	41.0	33.0	41.0
42	37.324522800859306	39.0	35.0	41.0	33.0	41.0
43	37.239993525358926	39.0	35.0	41.0	33.0	41.0
44	37.14095498999707	39.0	35.0	41.0	33.0	41.0
45	37.0677289511815	39.0	35.0	41.0	33.0	41.0
46	37.039339312758905	39.0	35.0	41.0	33.0	41.0
47	36.99887084998247	39.0	35.0	41.0	33.0	41.0
48	36.898371608209885	39.0	35.0	41.0	33.0	41.0
49	36.840396185438806	39.0	35.0	41.0	33.0	41.0
50	36.74515343285567	38.0	35.0	41.0	33.0	41.0
51	36.63273332058618	38.0	35.0	40.0	32.0	41.0
52	36.27942281802395	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	5.0
11	5.0
12	13.0
13	12.0
14	26.0
15	45.0
16	80.0
17	164.0
18	324.0
19	570.0
20	960.0
21	1582.0
22	2364.0
23	3320.0
24	4497.0
25	6040.0
26	7771.0
27	9548.0
28	11914.0
29	15392.0
30	19857.0
31	25621.0
32	33645.0
33	47679.0
34	107311.0
35	214461.0
36	120324.0
37	170128.0
38	303218.0
39	936387.0
40	1627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.327434922277412	13.695675242957972	46.19910694943179	11.777782885332835
2	26.22273645521226	12.858715409694652	48.37701189446335	12.54153624062974
3	26.296187443793123	13.551169469817854	45.36297845225759	14.789664634131434
4	23.836459564546157	14.018429254032347	47.31730289143582	14.827808289985677
5	23.47932736108007	16.905366078846846	43.82026318144497	15.79504337862811
6	25.049916841940025	19.37017977887438	43.41652725486466	12.163376124320934
7	86.05702672158702	2.9268409570927885	6.513371551972442	4.502760769347757
8	87.60712621295603	2.8461524543241947	6.4946420389055515	3.052079293814224
9	80.0721893138103	4.534596051349185	8.464321744671258	6.928892890169255
10	42.12614693816473	24.40827208749959	14.370524538840757	19.09505643549492
11	33.360343605876274	22.65219685451765	24.930497857842507	19.056961681763568
12	30.381470790028466	22.793181674809684	26.396338991471957	20.42900854368989
13	23.20190561792478	25.899395618663203	32.20087427215303	18.69782449125899
14	18.65479062311574	32.95406476890568	28.436339949953567	19.95480465802501
15	18.357270107452635	24.091875352400923	36.777037127958764	20.773817412187682
16	21.011530631556248	21.931624073732664	34.888486044067655	22.16835925064343
17	20.906342165219733	22.12840621624225	28.342105559144425	28.623146059393584
18	22.232518835875183	23.349638931175644	30.252662598336055	24.165179634613118
19	24.945217396832415	26.03646826912403	26.969031752637413	22.04928258140614
20	27.712930846040955	21.80658134550279	25.901058290841462	24.57942951761479
21	25.206501439433985	25.872646157442343	27.141167225210417	21.779685177913258
22	27.812495568245115	22.297607561441847	24.07358595844004	25.816310911872996
23	26.579379637449442	24.972602585650847	23.017593516752154	25.430424260147554
24	26.840565875805233	20.27482015021754	29.557029900225	23.327584073752224
25	28.400250183260706	23.410864389033993	23.815822868686553	24.37306255901875
26	23.187577295917993	27.486807429797334	23.750734143119885	25.574881131164783
27	21.421134812883363	25.858904660910238	24.092022058769594	28.62793846743681
28	21.781152241599962	26.23447296470587	27.952746856693793	24.031627937000373
29	21.732201216587015	23.483826356385958	26.28430422793084	28.499668199096188
30	22.154960068971555	25.291444426894017	27.37086049642501	25.182735007709418
31	23.151389724979353	27.380836529494584	24.6133186887776	24.854455056748467
32	26.03583254152646	23.594149545625925	23.155546405425007	27.214471507422612
33	24.715524125617815	25.063805044840805	24.951183455825003	25.26948737371638
34	23.149091325203518	23.07945470220808	26.83572456563912	26.93572940694928
35	21.571606645016068	23.6164000115409	27.17109532441913	27.6408980190239
36	23.810883754274656	26.132169723619874	22.42543771067646	27.63150881142901
37	26.245378138110354	27.144443667444047	24.57898939850878	22.03118879593682
38	22.38108348521518	24.189288381197915	29.469152785391568	23.960475348195338
39	22.386316012364414	26.092412297710254	25.24567203986892	26.275599650056407
40	25.06224017690832	22.676012188365107	27.831322885557785	24.430424749168786
41	22.513803846738792	23.439814445784908	26.953823192418607	27.092558515057696
42	22.111828396582524	26.01025673125496	23.808438648130153	28.069476224032364
43	21.673909886102066	22.955536722804673	26.787311463977964	28.5832419271153
44	26.19740515555521	23.03060148144091	24.45081693441394	26.321176428589943
45	21.38636540350853	23.95803024205084	26.357999727126153	28.297604627314477
46	21.122147233533557	25.910349694190575	24.38968928080137	28.577813791474497
47	22.327682367019236	24.250269328441817	28.331347092108615	25.090701212430332
48	20.899593672260906	21.042779088082995	31.733712292184318	26.32391494747178
49	20.835972010380942	22.006493223877342	27.6507762478477	29.50675851789402
50	20.386072479792418	22.343771165450065	30.807799497383982	26.462356857373535
51	22.02375567325753	23.105079414602468	27.833719089579397	27.037445822560603
52	21.5557134550768	22.246211430284397	27.95753926473702	28.240535849901782
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	195.0
1	175.0
2	155.0
3	144.0
4	133.0
5	290.0
6	447.0
7	478.0
8	509.0
9	596.5
10	684.0
11	1096.5
12	1509.0
13	1375.0
14	1593.0
15	1945.0
16	2082.5
17	2220.0
18	2523.0
19	2826.0
20	2839.5
21	2853.0
22	3275.0
23	3697.0
24	4410.0
25	5123.0
26	7112.0
27	9101.0
28	13200.5
29	17300.0
30	20112.0
31	22924.0
32	28594.0
33	34264.0
34	40101.5
35	45939.0
36	47706.5
37	49474.0
38	52233.5
39	70989.0
40	86985.0
41	97586.5
42	108188.0
43	112049.5
44	115911.0
45	124112.0
46	132313.0
47	146018.0
48	159723.0
49	180088.0
50	200453.0
51	204495.5
52	208538.0
53	181086.5
54	153635.0
55	137316.0
56	120997.0
57	116956.0
58	112915.0
59	107175.5
60	101436.0
61	95636.0
62	89836.0
63	75849.0
64	52858.5
65	43855.0
66	37771.5
67	31688.0
68	25520.0
69	19352.0
70	17646.5
71	15941.0
72	13163.5
73	10386.0
74	8594.0
75	6802.0
76	5170.5
77	3539.0
78	2506.0
79	1473.0
80	1096.0
81	719.0
82	551.5
83	384.0
84	316.5
85	249.0
86	154.5
87	60.0
88	49.0
89	37.5
90	37.0
91	38.0
92	39.0
93	21.0
94	3.0
95	6.5
96	10.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2044901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.04165460278658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93844369247478	10.898450479655446
2	12.503549280654273	4.011552377383528
3	5.388925862229241	2.5934186208571597
4	3.012500808968214	1.9330198987233296
5	1.8772785569641948	1.505732710201861
6	1.2674373104395649	1.2199074938853771
7	0.8740913728602905	0.9815310326290223
8	0.6240659479164892	0.8008840308669534
9	0.4767376729774283	0.6882894977436111
>10	3.136315481148348	10.375155201196007
>50	1.0944161521226454	13.117976434034103
>100	1.7916050481922023	47.83816425161234
>500	0.008535807613867888	0.9178120234547018
>1k	0.004572754078857798	1.6149735860277108
>5k	0.001524251359619266	1.5031323617288561
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	6946	0.3396741455943344	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	6361	0.3110664037036512	No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	6112	0.29888977510402703	No Hit
GTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	6055	0.2961023540992938	No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	5263	0.2573718727703689	No Hit
TTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	4922	0.24069624886485946	No Hit
ATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	4299	0.2102302263043541	No Hit
GGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	4072	0.1991294444083112	No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	3633	0.17766141245957628	No Hit
ACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2881	0.1408870160462536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36094656905150907	0.0
2	0.0	0.0	0.0	0.8614597968312402	0.0
3	0.0	0.0	0.0	3.1819144300873243	0.0
4	4.890212289005678E-5	0.0	0.0	3.665360816978426	0.0
5	4.890212289005678E-5	0.0	0.0	4.472392551033033	0.0
6	4.890212289005678E-5	0.0	0.0	6.979066468254453	0.0
7	4.890212289005678E-5	0.0	0.0	7.285242659669099	0.0
8	4.890212289005678E-5	0.0	0.0	7.88062600585554	0.0
9	4.890212289005678E-5	0.0	0.0	8.266463755458089	0.0
10	9.780424578011356E-5	0.0	0.0	9.633864915709855	0.0
11	9.780424578011356E-5	0.0	0.0	10.759934099499194	0.0
12	9.780424578011356E-5	0.0	0.0	11.21296336595268	0.0
13	1.4670636867017035E-4	0.0	0.0	11.444319309345538	0.0
14	1.4670636867017035E-4	0.0	0.0	11.568530701486281	0.0
15	1.4670636867017035E-4	0.0	0.0	11.779592263879767	0.0
16	1.4670636867017035E-4	0.0	0.0	12.267879960936984	0.0
17	1.4670636867017035E-4	0.0	0.0	12.949037630672585	0.0
18	1.4670636867017035E-4	0.0	0.0	13.684085439833028	0.0
19	1.4670636867017035E-4	0.0	0.0	14.128605736903644	0.0
20	1.4670636867017035E-4	0.0	0.0	14.617773672172882	0.0
21	1.4670636867017035E-4	0.0	0.0	15.378837410710837	0.0
22	1.4670636867017035E-4	0.0	0.0	16.032805500119565	0.0
23	1.4670636867017035E-4	0.0	0.0	16.727166743035482	0.0
24	1.9560849156022712E-4	0.0	0.0	17.2774134297944	0.0
25	2.445106144502839E-4	0.0	0.0	17.753524498251995	0.0
26	2.445106144502839E-4	0.0	0.0	18.18122246504843	0.0
27	2.445106144502839E-4	0.0	0.0	18.56847837621479	0.0
28	2.445106144502839E-4	0.0	0.0	18.960477793301486	0.0
29	2.445106144502839E-4	0.0	0.0	19.391745615068896	0.0
30	2.445106144502839E-4	0.0	0.0	19.847904617387346	0.0
31	2.445106144502839E-4	0.0	0.0	20.29932011378546	0.0
32	2.445106144502839E-4	0.0	0.0	20.732054999239573	0.0
33	2.445106144502839E-4	0.0	0.0	21.150852779670018	0.0
34	2.445106144502839E-4	0.0	0.0	21.56524936904036	0.0
35	2.445106144502839E-4	0.0	0.0	22.06693624776945	0.0
36	2.445106144502839E-4	0.0	0.0	22.51468408495081	0.0
37	2.445106144502839E-4	0.0	0.0	22.948201404371165	0.0
38	2.445106144502839E-4	0.0	0.0	23.379909345244588	0.0
39	2.934127373403407E-4	0.0	0.0	23.84946752923491	0.0
40	3.4231486023039747E-4	0.0	0.0	24.283571674129945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	30	1.8621195E-6	46.0	40
ACACGTT	20	6.3126144E-4	46.0	45
CTCCGAT	20	6.3126144E-4	46.0	41
TCCGATT	45	3.110472E-10	46.0	24
TCCGATG	25	3.4180972E-5	46.0	19
ACGTTAC	40	5.6152203E-9	46.0	13
GACCGTA	30	1.8621195E-6	46.0	38
GCTATCG	20	6.3126144E-4	46.0	30
CCAATCG	30	1.8621195E-6	46.0	21
CGATTCG	20	6.3126144E-4	46.0	16
TCTAGCG	65	0.0	46.0	1
CTGCGTA	20	6.3126144E-4	46.0	1
CGTTACA	45	3.110472E-10	46.0	37
CGTAGCG	25	3.4180972E-5	46.0	1
CTACGAT	20	6.3126144E-4	46.0	27
CACTACG	45	3.110472E-10	46.0	18
TACGAAC	20	6.3126144E-4	46.0	34
GTCGTTA	20	6.3126144E-4	46.0	35
GTCGTAA	20	6.3126144E-4	46.0	31
CAAATCG	20	6.3126144E-4	46.0	23
>>END_MODULE