FastQCFastQC Report
Thu 26 May 2016
SRR1545616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545616_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3086298
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC83380.2701618573449486No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68440.22175434776551067No Hit
ATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT46490.1506335421919724No Hit
ATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG38090.12341646853285068No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA35830.11609377966742034No Hit
ATGCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC33450.1083822754640025No Hit
ATTGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC31760.10290645945401254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA453.8562575E-1045.0000042
ACGGGTA1100.045.07
CGTTACG650.044.9999963
TACGCTA302.1666965E-644.9999962
TACACCG302.1666965E-644.9999963
GCGTTAG1350.040.03
TAGTACG850.039.7058833
ATCTCGA1950.039.230771
ATCGTAC1550.039.1935461
ATTCGAT1150.039.1304361
ATCGTTC2150.038.7209321
TAACGGC356.251714E-638.57142616
CGAATGT356.251714E-638.57142634
TAGTCGT356.251714E-638.5714262
ATCGTTA3750.037.81
AACACGT8000.037.687543
CATATCG301.1403678E-437.49999636
ATTAGTT16050.037.289721
TAGCGTA850.037.0588232
TACGGGT2550.037.0588236
Produced by FastQC (version 0.11.5)