##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545616_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3086298
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850290218248528	31.0	31.0	34.0	31.0	34.0
2	32.50422739476227	33.0	31.0	34.0	31.0	34.0
3	32.498081844332596	34.0	31.0	34.0	31.0	34.0
4	35.985788151371	37.0	35.0	37.0	35.0	37.0
5	35.76181950025565	37.0	35.0	37.0	35.0	37.0
6	35.7640759252671	37.0	35.0	37.0	35.0	37.0
7	35.74811732373219	37.0	35.0	37.0	33.0	37.0
8	35.713786549451804	37.0	35.0	37.0	33.0	37.0
9	37.87707343879301	39.0	38.0	39.0	35.0	39.0
10	37.78778880069261	39.0	38.0	39.0	35.0	39.0
11	37.61338503281277	39.0	37.0	39.0	35.0	39.0
12	37.156108386163616	39.0	37.0	39.0	33.0	39.0
13	36.923920178803215	39.0	37.0	39.0	33.0	39.0
14	37.75679308997381	40.0	36.0	41.0	33.0	41.0
15	37.72586250582413	40.0	36.0	41.0	33.0	41.0
16	37.62672010285462	40.0	36.0	41.0	32.0	41.0
17	37.73681024969073	40.0	36.0	41.0	32.0	41.0
18	37.7767370487231	40.0	36.0	41.0	33.0	41.0
19	37.74623383743242	40.0	36.0	41.0	33.0	41.0
20	37.705660308887865	40.0	36.0	41.0	33.0	41.0
21	37.688419264763155	40.0	36.0	41.0	33.0	41.0
22	37.61268322112771	40.0	35.0	41.0	32.0	41.0
23	37.562348483522975	40.0	35.0	41.0	32.0	41.0
24	37.544668078066344	40.0	35.0	41.0	32.0	41.0
25	37.471277562957304	40.0	35.0	41.0	32.0	41.0
26	37.37884805679815	40.0	35.0	41.0	32.0	41.0
27	37.263799542364346	39.0	35.0	41.0	32.0	41.0
28	37.17177051600331	39.0	35.0	41.0	31.0	41.0
29	37.12039569736947	39.0	35.0	41.0	31.0	41.0
30	37.05742089713955	39.0	35.0	41.0	31.0	41.0
31	36.941666358854526	39.0	35.0	41.0	31.0	41.0
32	36.83873851455692	39.0	35.0	41.0	31.0	41.0
33	36.726092878911885	39.0	35.0	41.0	30.0	41.0
34	36.65224096960177	39.0	35.0	41.0	30.0	41.0
35	36.60589158921141	39.0	35.0	41.0	30.0	41.0
36	36.484354394812165	39.0	35.0	41.0	30.0	41.0
37	36.35810864666989	39.0	35.0	41.0	30.0	41.0
38	36.27211759849502	39.0	35.0	41.0	30.0	41.0
39	36.20824981903886	38.0	35.0	41.0	30.0	41.0
40	36.129597984381284	38.0	35.0	41.0	30.0	41.0
41	36.04940222881912	38.0	35.0	40.0	29.0	41.0
42	35.92200623530197	38.0	35.0	40.0	29.0	41.0
43	35.790301519814356	38.0	35.0	40.0	29.0	41.0
44	35.72010803882191	38.0	35.0	40.0	29.0	41.0
45	35.696457049837704	38.0	35.0	40.0	29.0	41.0
46	35.622061771092746	38.0	34.0	40.0	29.0	41.0
47	35.55922338024391	38.0	34.0	40.0	29.0	41.0
48	35.48817871767405	37.0	34.0	40.0	28.0	41.0
49	35.44557265695017	37.0	34.0	40.0	28.0	41.0
50	35.35977698848264	37.0	34.0	40.0	28.0	41.0
51	35.15081596138805	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	22.0
10	40.0
11	56.0
12	50.0
13	70.0
14	80.0
15	116.0
16	258.0
17	493.0
18	1013.0
19	1874.0
20	3184.0
21	5326.0
22	8163.0
23	11414.0
24	16359.0
25	22676.0
26	29535.0
27	36217.0
28	42840.0
29	50897.0
30	61512.0
31	75846.0
32	94461.0
33	122366.0
34	195027.0
35	297172.0
36	233362.0
37	320487.0
38	493476.0
39	961474.0
40	425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3823039771272896	90.36450789910761	3.6694123509784218	3.5837757727866846
2	3.6097615978755133	3.0954561095526096	90.05407125300279	3.2407110395690895
3	29.25190632920087	15.818563210681535	41.4844580789023	13.445072381215295
4	28.09731918304713	16.028069875300442	41.95978482959195	13.914826112060469
5	25.40457855981503	14.103984773991366	45.24776933400469	15.24366733218892
6	23.773303809288667	14.85452798141981	46.069724958510164	15.302443250781355
7	23.699266888680228	17.224487071566	42.991149914881845	16.085096124871935
8	26.817501096783264	19.203038721471486	42.66966443292255	11.3097957488227
9	81.72052731136138	4.254320224424212	9.341418100261219	4.683734363953189
10	81.60790046845769	4.663580768934173	9.583034431542256	4.145484331065892
11	74.82540571260455	7.657134858655905	10.584622742197935	6.93283668654161
12	40.32825086884028	24.64923996321807	17.049844182253302	17.972664985688354
13	32.02480771461473	24.254592395160802	25.58304479995127	18.137555090273203
14	27.09100028577927	23.854695820040707	28.792715415037694	20.261588479142326
15	23.77113292365157	24.27267230837722	32.74826345349671	19.207931314474493
16	19.859002597934484	29.326072854921982	31.49699089329676	19.31793365384678
17	20.0587888791037	23.789018429198993	35.88658645406244	20.265606237634863
18	24.353189484618788	22.781597888473502	32.338549291092434	20.52666333581527
19	24.383646686094472	22.446665876075482	29.278151364515026	23.89153607331502
20	24.493811031857586	22.889040526870705	30.78610685034303	21.831041590928677
21	25.819217716500482	24.84287000153582	28.08393745516473	21.25397482679897
22	25.535414921047806	24.436298763113605	27.705911742806432	22.322374573032157
23	26.440998244498747	24.457165186252265	29.055748991186203	20.046087578062778
24	28.396026566455994	20.97286133743404	27.626269401075334	23.004842695034633
25	27.44261895643259	23.928279122754834	25.96768037305536	22.661421547757215
26	26.242281205508995	22.927274035106137	28.1985731773147	22.631871582070172
27	27.34178617878118	23.980283174210655	25.888102833880588	22.789827813127573
28	23.260618384874046	25.95173894419787	26.290688715088432	24.49695395583965
29	22.31090452056153	24.471616156314134	28.46951266533562	24.747966657788716
30	22.71919302672652	24.84163875296553	29.305530444564976	23.133637775742976
31	22.598692673228573	24.768962686040037	28.837267172515425	23.79507746821597
32	23.68096016651665	24.467663200377928	28.625946036319238	23.225430596786182
33	24.701730033846374	26.228121846950618	27.49050804556138	21.579640073641627
34	25.169442484167114	25.379305562845843	26.62717598883841	22.824075964148633
35	25.045053977289296	24.815264112538713	25.3785927347262	24.761089175445793
36	22.738180175731575	26.17527536226249	27.858780973191834	23.22776348881411
37	22.55987594198616	25.52893466541468	29.240922295902728	22.67026709669643
38	24.836551752293527	27.195040789969084	24.86402803617797	23.104379421559422
39	23.768281611173	27.983720301798463	25.809950950945115	22.438047136083426
40	23.345185720886317	26.125020979827614	27.512929730051994	23.01686356923408
41	22.711449121244932	26.875272575752568	25.608155790529626	24.80512251247287
42	24.548471988122987	24.26719649236723	27.539142364087976	23.645189155421804
43	22.276591566984134	24.95186790128497	28.105225094919543	24.666315436811352
44	21.191083945879498	26.132343668693043	27.13273313205659	25.543839253370866
45	21.66433701476656	23.844003398246056	28.088797646889574	26.40286194009781
46	23.744628678112097	24.12135833934377	27.409245639922002	24.72476734262213
47	21.175110115743845	25.553462432986056	27.361064939289726	25.910362511980374
48	21.4348063602413	25.8847979035077	27.35017810982608	25.33021762642493
49	22.43503381721402	25.848443669405867	28.979930000278653	22.736592513101456
50	21.53256101646698	25.31835875861631	29.86652617472454	23.28255405019217
51	22.08824293700738	25.386271837651453	27.567299074813906	24.95818615052727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	682.0
1	1953.0
2	3224.0
3	7809.0
4	12394.0
5	8696.5
6	4999.0
7	5208.0
8	5417.0
9	5714.5
10	6012.0
11	6280.0
12	6548.0
13	7309.0
14	8070.0
15	7669.5
16	7269.0
17	8220.5
18	9172.0
19	9231.0
20	9290.0
21	10735.5
22	12181.0
23	15516.5
24	18852.0
25	19495.5
26	25949.0
27	31759.0
28	38348.0
29	44937.0
30	55011.5
31	65086.0
32	73922.5
33	82759.0
34	91278.0
35	99797.0
36	108762.5
37	117728.0
38	129876.0
39	142024.0
40	157892.0
41	173760.0
42	204866.0
43	235972.0
44	251460.0
45	266948.0
46	265306.5
47	263665.0
48	244602.5
49	225540.0
50	213478.0
51	201416.0
52	192075.0
53	182734.0
54	179442.0
55	176150.0
56	170434.0
57	164718.0
58	151534.5
59	138351.0
60	123951.0
61	109551.0
62	94070.0
63	78589.0
64	68112.5
65	57636.0
66	47283.0
67	36930.0
68	31706.5
69	26483.0
70	21805.5
71	17128.0
72	13490.0
73	9852.0
74	8133.0
75	4869.0
76	3324.0
77	2472.5
78	1621.0
79	1120.5
80	620.0
81	478.0
82	336.0
83	227.0
84	118.0
85	94.0
86	70.0
87	44.5
88	19.0
89	14.0
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3086298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.23860191107383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4579515196255	11.92854431930159
2	10.233603718625409	3.323588338048856
3	3.9330207896264118	1.9160027673216162
4	2.1256233947432963	1.3806860848040692
5	1.325780475841486	1.076441068433196
6	0.9566314973248554	0.9320614836391689
7	0.6985991018526548	0.7940990897293286
8	0.5325441999704114	0.6918218610696643
9	0.406745657392902	0.5944482728515224
>10	3.223625737118127	11.603767149444923
>50	1.3334548547343936	15.954882132764759
>100	1.7603563198623426	46.87620208244444
>500	0.007639731078766696	0.8729804400906266
>1k	0.004020911094087735	1.5588283490443995
>5k	4.020911094087735E-4	0.4956465610118135
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	8338	0.2701618573449486	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6844	0.22175434776551067	No Hit
ATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	4649	0.1506335421919724	No Hit
ATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG	3809	0.12341646853285068	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	3583	0.11609377966742034	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	3345	0.1083822754640025	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	3176	0.10290645945401254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0853125654100803	0.0
2	0.0	0.0	0.0	0.46165341130376913	0.0
3	0.0	0.0	0.0	0.6433273779784064	0.0
4	3.2401278165621075E-5	0.0	0.0	0.9124523944220552	0.0
5	3.2401278165621075E-5	0.0	0.0	1.9082732775642532	0.0
6	3.2401278165621075E-5	0.0	0.0	2.1451266209549433	0.0
7	3.2401278165621075E-5	0.0	0.0	2.530475022178675	0.0
8	6.480255633124215E-5	0.0	0.0	3.720314758976612	0.0
9	6.480255633124215E-5	0.0	0.0	3.865245676211435	0.0
10	6.480255633124215E-5	0.0	0.0	4.124715111761729	0.0
11	9.720383449686324E-5	0.0	0.0	4.334675394274953	0.0
12	9.720383449686324E-5	0.0	0.0	4.981048492400928	0.0
13	1.296051126624843E-4	0.0	0.0	5.525519570696025	0.0
14	1.296051126624843E-4	0.0	0.0	5.747597931243192	0.0
15	1.296051126624843E-4	0.0	0.0	5.869685947371252	0.0
16	1.9440766899372647E-4	0.0	0.0	5.983706045236072	0.0
17	2.592102253249686E-4	0.0	0.0	6.117912139398075	0.0
18	2.592102253249686E-4	0.0	0.0	6.394845863879638	0.0
19	2.592102253249686E-4	0.0	0.0	6.736193329354457	0.0
20	3.240127816562108E-4	0.0	0.0	7.102813791798459	0.0
21	3.5641405982183183E-4	0.0	0.0	7.359075500810356	0.0
22	3.8881533798745295E-4	0.0	0.0	7.650946214526271	0.0
23	3.8881533798745295E-4	0.0	0.0	8.080230748942585	0.0
24	3.8881533798745295E-4	0.0	0.0	8.466194774451463	0.0
25	3.8881533798745295E-4	0.0	0.0	8.855917348227553	0.0
26	3.8881533798745295E-4	0.0	0.0	9.16337307674113	0.0
27	0.001296051126624843	0.0	0.0	9.437876705360273	0.0
28	0.001296051126624843	0.0	0.0	9.697410943466897	0.0
29	0.0013608536829560853	0.0	0.0	9.95947248127044	0.0
30	0.0014256562392873273	0.0	0.0	10.219719547496709	0.0
31	0.0014580575174529486	0.0	0.0	10.525296001876683	0.0
32	0.0014904587956185696	0.0	0.0	10.86537981750304	0.0
33	0.0015228600737841906	0.0	0.0	11.166031277601839	0.0
34	0.0015228600737841906	0.0	0.0	11.453949035381548	0.0
35	0.001652465186446675	0.0	0.0	11.759039470589036	0.0
36	0.001717267742777917	0.0	0.0	12.087296819684942	0.0
37	0.0017496690209435383	0.0	0.0	12.460721550543726	0.0
38	0.0017496690209435383	0.0	0.0	12.766524813870857	0.0
39	0.0018792741336060225	0.0	0.0	13.096499430709542	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	45	3.8562575E-10	45.000004	2
ACGGGTA	110	0.0	45.0	7
CGTTACG	65	0.0	44.999996	3
TACGCTA	30	2.1666965E-6	44.999996	2
TACACCG	30	2.1666965E-6	44.999996	3
GCGTTAG	135	0.0	40.0	3
TAGTACG	85	0.0	39.705883	3
ATCTCGA	195	0.0	39.23077	1
ATCGTAC	155	0.0	39.193546	1
ATTCGAT	115	0.0	39.130436	1
ATCGTTC	215	0.0	38.720932	1
TAACGGC	35	6.251714E-6	38.571426	16
CGAATGT	35	6.251714E-6	38.571426	34
TAGTCGT	35	6.251714E-6	38.571426	2
ATCGTTA	375	0.0	37.8	1
AACACGT	800	0.0	37.6875	43
CATATCG	30	1.1403678E-4	37.499996	36
ATTAGTT	1605	0.0	37.28972	1
TAGCGTA	85	0.0	37.058823	2
TACGGGT	255	0.0	37.058823	6
>>END_MODULE