FastQCFastQC Report
Thu 26 May 2016
SRR1545615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545615_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2464192
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT67410.2735582292288913No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT62110.2520501649222138Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC57930.23508720099732489No Hit
GTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC57580.233666857128016No Hit
GCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC50360.20436719216684415No Hit
GGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC46190.187444809495364No Hit
ATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC41660.16906150170116616No Hit
TTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC39820.1615945510739423No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC37350.15157098148196244No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC29740.1206886476378464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA206.3128665E-446.00000428
TAACGAC206.3128665E-446.0000048
ACCGTAT206.3128665E-446.00000410
ACTACGC301.8622777E-646.030
GCGTAAT301.8622777E-646.06
CCGTCTA351.0202166E-745.99999630
CGGTTAG650.042.4615361
ACGGGTA451.5896148E-840.8888855
TATCGGA451.5896148E-840.88888513
CGATACG451.5896148E-840.8888851
TTTAGCG2650.040.7924541
CGTTACG800.040.2500041
TAACGGC355.3756175E-639.4285714
ACTAACG355.3756175E-639.4285719
ACGACCA6350.039.11810728
TAGTACG950.038.7368431
TTTGGGC51250.038.5502434
ACACGAC6350.038.393726
CAACCGT301.0018991E-438.33333235
CGTCCTA301.0018991E-438.33333233