##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545615_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2464192
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.529776494688726	33.0	31.0	34.0	31.0	34.0
2	32.945083013011974	34.0	31.0	34.0	31.0	34.0
3	33.0325749779238	34.0	33.0	34.0	31.0	34.0
4	36.40898355322962	37.0	37.0	37.0	35.0	37.0
5	36.38999923707244	37.0	37.0	37.0	35.0	37.0
6	36.39787200023375	37.0	37.0	37.0	35.0	37.0
7	36.46986273796847	37.0	37.0	37.0	35.0	37.0
8	36.49355975508402	37.0	37.0	37.0	35.0	37.0
9	38.28642573305976	39.0	39.0	39.0	37.0	39.0
10	38.04599114030076	39.0	39.0	39.0	35.0	39.0
11	37.8854845726307	39.0	38.0	39.0	35.0	39.0
12	37.58020722411241	39.0	37.0	39.0	35.0	39.0
13	37.519404332129966	39.0	37.0	39.0	35.0	39.0
14	38.71874715931226	41.0	38.0	41.0	35.0	41.0
15	38.83003394216035	41.0	38.0	41.0	35.0	41.0
16	38.90910692023998	41.0	38.0	41.0	35.0	41.0
17	38.86849076695322	41.0	38.0	41.0	35.0	41.0
18	38.85515941939589	41.0	38.0	41.0	35.0	41.0
19	38.82698344934161	40.0	38.0	41.0	35.0	41.0
20	38.72438470703582	40.0	38.0	41.0	35.0	41.0
21	38.698533636989325	40.0	38.0	41.0	35.0	41.0
22	38.81693390774745	40.0	38.0	41.0	35.0	41.0
23	38.81362572396956	40.0	38.0	41.0	35.0	41.0
24	38.77156284899878	41.0	38.0	41.0	35.0	41.0
25	38.67168873204685	40.0	38.0	41.0	35.0	41.0
26	38.56402666675324	40.0	37.0	41.0	35.0	41.0
27	38.486893066774016	40.0	37.0	41.0	35.0	41.0
28	38.44120912656157	40.0	37.0	41.0	35.0	41.0
29	38.364476469366025	40.0	37.0	41.0	34.0	41.0
30	38.318563245071815	40.0	37.0	41.0	34.0	41.0
31	38.24584975521388	40.0	37.0	41.0	34.0	41.0
32	38.17809001895956	40.0	36.0	41.0	34.0	41.0
33	38.161553564008	40.0	36.0	41.0	34.0	41.0
34	38.028768050541515	40.0	36.0	41.0	34.0	41.0
35	37.9541131535205	40.0	36.0	41.0	34.0	41.0
36	37.86155908305847	40.0	35.0	41.0	34.0	41.0
37	37.76421561306911	40.0	35.0	41.0	33.0	41.0
38	37.698112809391475	40.0	35.0	41.0	33.0	41.0
39	37.593234212269174	40.0	35.0	41.0	33.0	41.0
40	37.45359898903982	40.0	35.0	41.0	33.0	41.0
41	37.29631294964029	39.0	35.0	41.0	33.0	41.0
42	37.226155267122046	39.0	35.0	41.0	33.0	41.0
43	37.14731563124951	39.0	35.0	41.0	33.0	41.0
44	37.025451344700414	39.0	35.0	41.0	33.0	41.0
45	36.98628678284809	39.0	35.0	41.0	33.0	41.0
46	36.965052236189386	39.0	35.0	41.0	33.0	41.0
47	36.93099969482898	39.0	35.0	41.0	33.0	41.0
48	36.84190436459497	39.0	35.0	41.0	33.0	41.0
49	36.76017940160507	39.0	35.0	41.0	32.0	41.0
50	36.66267928797756	38.0	35.0	41.0	32.0	41.0
51	36.55279621068488	38.0	35.0	40.0	32.0	41.0
52	36.20978073137158	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	7.0
11	5.0
12	16.0
13	34.0
14	45.0
15	63.0
16	120.0
17	234.0
18	504.0
19	835.0
20	1362.0
21	2242.0
22	3216.0
23	4450.0
24	5910.0
25	7611.0
26	9342.0
27	11883.0
28	15121.0
29	19341.0
30	24984.0
31	32211.0
32	42520.0
33	61569.0
34	142439.0
35	214420.0
36	148268.0
37	220736.0
38	384399.0
39	1108735.0
40	1561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.575330169077734	13.468633937615252	45.84845661377035	12.10757927953666
2	26.69824429265252	12.988030153494535	48.169501402488116	12.144224151364828
3	26.717155156741036	13.78098784510298	44.36995980832663	15.131897189829363
4	24.279195776952445	14.416246785964729	46.07047665117004	15.234080785912784
5	23.951299249409136	16.612382476690126	43.39276322624211	16.043555047658625
6	26.36357069578994	18.719442316183155	43.520513011973094	11.396473976053814
7	86.78491773368309	2.6299087084123314	6.521691491572085	4.063482066332494
8	87.11342298002754	2.910162844453679	6.848411162766538	3.1280030127522527
9	79.49875659039556	5.189693010934213	8.575386982832507	6.736163415837727
10	42.385293029114614	23.87565579305509	14.926596628834115	18.812454548996183
11	33.304628860088826	23.14831798820871	25.26767394748461	18.279379204217854
12	28.134780082071526	23.59532049450692	27.477404358101964	20.792495065319585
13	24.689147598888397	22.94354498350778	33.29566040308548	19.07164701451835
14	19.762705178817235	29.58040607225411	31.460900773965665	19.19598797496299
15	19.942114900137653	23.321884009038257	35.62413156117705	21.11186952964704
16	24.667883022102174	23.007095226346	31.1975690205958	21.12745273095603
17	24.91116763628808	22.69007447471626	27.652918279095136	24.74583960990053
18	24.97824844817287	22.66568514141755	29.33549820793185	23.02056820247773
19	26.244342973274808	25.06387489286549	27.09975521387944	21.592026919980263
20	25.92095096485988	23.918590759161624	26.159203503623097	24.001254772355402
21	26.35995896423655	24.919933187024387	28.62828870477625	20.09181914396281
22	28.817519089421605	20.50558560371919	26.995014998831262	23.681880308027946
23	27.78687699659767	23.64101498584526	24.80147650832403	23.77063150923305
24	26.855253162091262	22.15789191751292	27.11306586499753	23.87378905539828
25	29.337405526842065	23.6136226397943	23.547799846765187	23.501171986598447
26	23.440705919019297	25.394571526894012	25.277210542035682	25.887512012051012
27	22.82395202971197	22.904384073968263	27.17219275121419	27.099471145105575
28	23.134601524556526	23.501537217879125	28.665542295405555	24.698318962158794
29	23.003361751032386	24.65668259616134	27.041074721450276	25.298880931356
30	23.96164746902839	23.9851440147521	27.19012966522089	24.863078850998622
31	24.18066449367582	26.37363484663533	26.547525517492144	22.89817514219671
32	25.565175116224708	25.39558605822923	25.213579136690644	23.825659688855414
33	25.26040990312443	23.951339830662548	24.093090148819574	26.695160117393453
34	21.8349868841389	26.65185992000623	27.123860478404282	24.38929271745059
35	22.13719547827442	24.65246214580682	29.46767135028439	23.742671025634365
36	24.48352238786588	26.223484209022672	24.272946263927487	25.02004713918396
37	24.063749902604993	28.11051249253305	24.628803275069476	23.196934329792484
38	22.894401085629692	25.39647884580422	27.899530556060565	23.809589512505518
39	22.78897098927356	26.472084967405134	24.927643625172063	25.811300418149237
40	25.33572870945121	23.02783224683791	27.479758070799676	24.1566809729112
41	22.741125691504557	24.006570916551954	27.721744084876505	25.53055930706698
42	21.09084844038127	25.708345778251047	26.30168428434148	26.899121497026208
43	21.490654948965016	22.605462561358856	28.182463054826894	27.72141943484923
44	25.60287510064151	22.61179323689063	25.90759973248838	25.87773192997948
45	21.10736501051866	23.702982559800535	26.94964515752019	28.24000727216061
46	21.304062345791237	24.83442848609199	26.870308807105943	26.99120036101083
47	22.596169454328237	24.780090187777574	29.387685699815595	23.236054658078594
48	21.019466015635146	24.16913941770771	30.89284438874893	23.918550177908216
49	22.181307300729813	23.844570552943928	26.68063202867309	27.293490117653167
50	21.025390878632834	23.734473612445782	29.584017803807495	25.65611770511389
51	22.29120133496091	24.155504116562344	27.626012908085084	25.92728164039166
52	21.601644677038152	23.329148053398434	27.743373892943406	27.32583337662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	149.5
2	92.0
3	101.5
4	111.0
5	201.0
6	291.0
7	428.0
8	565.0
9	802.0
10	1039.0
11	1186.0
12	1333.0
13	1449.5
14	1937.0
15	2308.0
16	2549.0
17	2790.0
18	2989.0
19	3188.0
20	3301.0
21	3414.0
22	4989.5
23	6565.0
24	8465.0
25	10365.0
26	12191.0
27	14017.0
28	16959.5
29	19902.0
30	25245.0
31	30588.0
32	38365.0
33	46142.0
34	50681.0
35	55220.0
36	58639.0
37	62058.0
38	71639.5
39	91837.0
40	102453.0
41	114109.0
42	125765.0
43	144401.5
44	163038.0
45	188754.5
46	214471.0
47	226923.5
48	239376.0
49	221270.0
50	203164.0
51	187209.0
52	171254.0
53	162248.0
54	153242.0
55	146666.0
56	140090.0
57	133633.5
58	127177.0
59	122148.5
60	117120.0
61	107791.5
62	98463.0
63	89243.5
64	69508.5
65	58993.0
66	51760.5
67	44528.0
68	36055.5
69	27583.0
70	24502.0
71	21421.0
72	17579.0
73	13737.0
74	11236.5
75	8736.0
76	6717.5
77	4699.0
78	3702.5
79	2706.0
80	2230.5
81	1755.0
82	1248.0
83	741.0
84	517.5
85	294.0
86	264.0
87	234.0
88	145.0
89	51.5
90	47.0
91	32.0
92	17.0
93	13.5
94	10.0
95	8.0
96	6.0
97	5.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2464192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.195443247292992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.14426967815542	12.061618083735363
2	11.819858477246248	4.064954112730456
3	4.759696680510334	2.455352824321329
4	2.5223674704145633	1.734929067453264
5	1.4999300483894744	1.2895981010995319
6	0.9712139134418586	1.0020272237742485
7	0.6763905190624886	0.814158434848225
8	0.5252524160754296	0.7225558488902858
9	0.41707728547996464	0.6454645912985174
>10	3.5251402619541676	13.88965614505779
>50	1.5674929538999387	19.802012888468333
>100	1.5623178575849197	38.55050582627375
>500	0.004969505091774537	0.5853519729932867
>1k	0.002839717195299735	1.1798189158823327
>5k	0.0011832154980415564	1.2019959631733539
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	6741	0.2735582292288913	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT	6211	0.2520501649222138	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	5793	0.23508720099732489	No Hit
GTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	5758	0.233666857128016	No Hit
GCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	5036	0.20436719216684415	No Hit
GGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	4619	0.187444809495364	No Hit
ATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	4166	0.16906150170116616	No Hit
TTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	3982	0.1615945510739423	No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	3735	0.15157098148196244	No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	2974	0.1206886476378464	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26475209723917614	0.0
2	0.0	0.0	0.0	0.686147832636418	0.0
3	0.0	0.0	0.0	2.523869893255071	0.0
4	0.0	0.0	0.0	2.9313462587330856	0.0
5	0.0	0.0	0.0	3.6083227280991093	0.0
6	0.0	0.0	0.0	5.738270394514713	0.0
7	0.0	0.0	0.0	5.99194380957328	0.0
8	0.0	0.0	0.0	6.459886242630445	0.0
9	0.0	0.0	0.0	6.783481157312417	0.0
10	0.0	0.0	0.0	7.94410500480482	0.0
11	0.0	0.0	0.0	8.903567579149676	0.0
12	0.0	0.0	0.0	9.298260849803912	0.0
13	0.0	0.0	0.0	9.488140534503804	0.0
14	0.0	0.0	0.0	9.617716476638185	0.0
15	0.0	0.0	0.0	9.81197893670623	0.0
16	0.0	0.0	0.0	10.253787042568112	0.0
17	0.0	0.0	0.0	10.815999727293978	0.0
18	4.0581253408825285E-5	0.0	0.0	11.422608303249097	0.0
19	8.116250681765057E-5	0.0	0.0	11.82533666207828	0.0
20	8.116250681765057E-5	0.0	0.0	12.28337726930369	0.0
21	8.116250681765057E-5	0.0	0.0	12.961490013765161	0.0
22	8.116250681765057E-5	0.0	0.0	13.569356608575955	0.0
23	1.2174376022647586E-4	0.0	0.0	14.155674557826663	0.0
24	1.6232501363530114E-4	0.0	0.0	14.614810858894112	0.0
25	0.0017044126431706621	0.0	0.0	15.030525218814118	0.0
26	0.0017044126431706621	0.0	0.0	15.407322156715061	0.0
27	0.0017449938965794873	0.0	0.0	15.79085558268187	0.0
28	0.0017449938965794873	0.0	0.0	16.16838298314417	0.0
29	0.0017449938965794873	0.0	0.0	16.58746558709711	0.0
30	0.0017449938965794873	0.0	0.0	17.040474118899827	0.0
31	0.0017449938965794873	0.0	0.0	17.443973521543775	0.0
32	0.0017449938965794873	0.0	0.0	17.84970489312521	0.0
33	0.0017449938965794873	0.0	0.0	18.277025491520142	0.0
34	0.0017449938965794873	0.0	0.0	18.710068046645716	0.0
35	0.0017449938965794873	0.0	0.0	19.199559125262965	0.0
36	0.0017449938965794873	0.0	0.0	19.60123237150352	0.0
37	0.0017449938965794873	0.0	0.0	20.010859543412202	0.0
38	0.0017449938965794873	0.0	0.0	20.480506389112538	0.0
39	0.0017449938965794873	0.0	0.0	21.287870425681117	0.0
40	0.0017449938965794873	0.0	0.0	21.738444082279305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	20	6.3128665E-4	46.000004	28
TAACGAC	20	6.3128665E-4	46.000004	8
ACCGTAT	20	6.3128665E-4	46.000004	10
ACTACGC	30	1.8622777E-6	46.0	30
GCGTAAT	30	1.8622777E-6	46.0	6
CCGTCTA	35	1.0202166E-7	45.999996	30
CGGTTAG	65	0.0	42.461536	1
ACGGGTA	45	1.5896148E-8	40.888885	5
TATCGGA	45	1.5896148E-8	40.888885	13
CGATACG	45	1.5896148E-8	40.888885	1
TTTAGCG	265	0.0	40.792454	1
CGTTACG	80	0.0	40.250004	1
TAACGGC	35	5.3756175E-6	39.42857	14
ACTAACG	35	5.3756175E-6	39.42857	19
ACGACCA	635	0.0	39.118107	28
TAGTACG	95	0.0	38.736843	1
TTTGGGC	5125	0.0	38.550243	4
ACACGAC	635	0.0	38.3937	26
CAACCGT	30	1.0018991E-4	38.333332	35
CGTCCTA	30	1.0018991E-4	38.333332	33
>>END_MODULE