FastQCFastQC Report
Thu 26 May 2016
SRR1545614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545614_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1384658
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC78850.5694546956721444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.2689472779560007No Hit
ATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG35070.2532755380751059No Hit
ATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT31470.22727633827269983No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT28670.20705473842638397Illumina Single End Adapter 1 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC24810.17917781863824858No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC24390.1761445786613012No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC22540.16278387876284253No Hit
ATATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC21190.15303417883694026No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC20380.14718435888139886No Hit
ATTGGTGGGTTGGTGAGACAATCAGATCGATAAATTGATTAAATGCTCCTA19350.13974569893793268No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC18950.13685689895988756No Hit
ATAGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC18310.13223481899501538No Hit
ATTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC17600.1271071990339853No Hit
ATGTTTCGGGGTAACAAAACTATTTGCCAGAGAACTACTAGCCATAGCTTA13910.10045801923651905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA406.8175723E-945.00000415
TCGCCAC207.0337864E-445.00000418
AACCGTA351.2121745E-745.00000420
CGGCGAA406.8175723E-945.00000439
CCTTAGT207.0337864E-445.00000421
CTGTCGT207.0337864E-445.00000439
TCGTTCG351.2121745E-745.0000043
CTCACCG406.8175723E-945.00000421
GTCGCAT207.0337864E-445.00000444
ACCGGTG351.2121745E-745.00000422
TCGCACA750.045.00000426
AAATCCG207.0337864E-445.00000421
GTCGACT207.0337864E-445.00000437
CTCCGAC351.2121745E-745.00000421
GGTCGAC207.0337864E-445.00000411
ACGCATA302.1658252E-645.00000426
CCCTATT406.8175723E-945.00000431
TCCGACA207.0337864E-445.00000420
CGGCCAG406.8175723E-945.00000424
TAGCGCA406.8175723E-945.0000043